Reviewed, UniProtKB/Swiss-Prot Q5P0D6 (FABH_AZOSE)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] synthase 3
    EC=2.3.1.41
Alternative name(s):
    3-oxoacyl-[acyl-carrier-protein] synthase III
    Beta-ketoacyl-ACP synthase III
      Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	AZOSEA31030
ORF Names:	ebA5455

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity.
Catalytic activity	Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein]. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/acpP and fabH By similarity.
Sequence similarities	Belongs to the fabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000056325
Regions
	Region	249 – 253	5	ACP-binding By similarity
Sites
	Active site	115	1	By similarity
	Active site	248	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P0D6-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 47C637CDFAFD17F2	FASTA	321	33,695
	10 20 30 40 50 60 MIYARIAGTG SFLPGEPITN DDLVARGIDT SDQWIIERTG IRSRHLASAE MSSSDLAKTA 70 80 90 100 110 120 SERALDAAGV RPDDIDLIIV ATSTPDCIFP STAALLQSKL GIRNGAAAFD VQAVCSGFVY 130 140 150 160 170 180 GLTIAEKFIR SGSHKRALVV GAEVFSRILD WSDRGTCVLF GDGAGAVVLE AAEAPGILAS 190 200 210 220 230 240 ALHADGSHHP ILCVPGAVAA GQVVGDPFLR MDGQAVFKFA VRVLGDVALE VLDQAGVTVD 250 260 270 280 290 300 SVDWLIPHQA NIRILQATAR RLGVSMDKVI TTVDHHGNTS AASIPLALDL AVRDGRIRPG 310 320 HRFIIEGVGG GFTWGAALLQ F

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI09228.1.
RefSeq	YP_160129.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P0D6.
Genome annotation databases
GeneID	3180040.
GenomeReviews	Gene locus AZOSEA31030 in contig CR555306_GR.
KEGG	eba:ebA5455.
NMPDR	fig|76114.4.peg.3107.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P0D6.
OMA	FKSAVTS.
Enzyme and pathway databases
BioCyc	ASP76114:EBA5455-MON.
Family and domain databases
HAMAP	MF_01815. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00747. fabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_AZOSE

Accession

Primary (citable) accession number: Q5P0D6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)