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Q5P081 (NAGZ_AROAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:nagZ
Ordered Locus Names:AZOSEA31580
ORF Names:ebA5547
OrganismAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]
Taxonomic identifier76114 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides By similarity. HAMAP-Rule MF_00364

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364

Pathway

Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00364.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Beta-hexosaminidase HAMAP-Rule MF_00364
PRO_0000234910

Regions

Region180 – 1812Substrate binding By similarity

Sites

Active site1931Proton donor/acceptor By similarity
Active site2641Nucleophile By similarity
Binding site751Substrate By similarity
Binding site831Substrate By similarity
Binding site1501Substrate By similarity
Site1911Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5P081 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: E83DCCB7854CC163

FASTA35638,840
        10         20         30         40         50         60 
MTLQSDLPAT VRPLGPVMLD VAGFALTEEE RERLLDPLVG GVILFARNFR DSEQLQALTA 

        70         80         90        100        110        120 
EIHALRSPAL IIAVDHEGGR VQRFRTDGFT RIPSMRCLGR LWERDHVAAL ESARCAGYVL 

       130        140        150        160        170        180 
AAELLAHGVD LSFTPVLDLD YGCSRVVGDR AFHRDPLVVA ALAQSLVSGM ADAGMGCVGK 

       190        200        210        220        230        240 
HFPGHGYAEA DSHVEIPVDE REFDAIWTED IAPYRHRLGR QLAGVMPAHV IYPRVDPNPA 

       250        260        270        280        290        300 
GFSRFWLQDI LRGRVGFGGV IFSDDLTMEG ATVVGDILAR ARAAFGAGCD VVLVCNRPDL 

       310        320        330        340        350 
AVDLLDRWAP DIAPESRARI EALRSRPQAA DPFALELHPV YRQARDVVAG LVEDTA 

« Hide

References

[1]"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EbN1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR555306 Genomic DNA. Translation: CAI09283.1.
RefSeqYP_160184.1. NC_006513.1.

3D structure databases

ProteinModelPortalQ5P081.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING76114.ebA5547.

Protein family/group databases

CAZyGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI09283; CAI09283; ebA5547.
GeneID3182301.
KEGGeba:ebA5547.
PATRIC20972534. VBIAroAro98752_3193.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000248526.
KOK01207.
OMADSHVDIP.
OrthoDBEOG6BCT06.
ProtClustDBPRK05337.

Enzyme and pathway databases

BioCycAARO76114:GJTA-3201-MONOMER.
UniPathwayUPA00544.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
HAMAPMF_00364. NagZ.
InterProIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAGZ_AROAE
AccessionPrimary (citable) accession number: Q5P081
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: January 4, 2005
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries