Reviewed, UniProtKB/Swiss-Prot Q5P081 (NAGZ_AZOSE)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase
    Beta-N-acetylhexosaminidase

Gene names

Name:	nagZ
Ordered Locus Names:	AZOSEA31580
ORF Names:	ebA5547

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP MF_00364
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP MF_00364
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Beta-hexosaminidase HAMAP MF_00364		PRO_0000234910
Sites
	Active site	264	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5P081-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: E83DCCB7854CC163	FASTA	356	38,840
	10 20 30 40 50 60 MTLQSDLPAT VRPLGPVMLD VAGFALTEEE RERLLDPLVG GVILFARNFR DSEQLQALTA 70 80 90 100 110 120 EIHALRSPAL IIAVDHEGGR VQRFRTDGFT RIPSMRCLGR LWERDHVAAL ESARCAGYVL 130 140 150 160 170 180 AAELLAHGVD LSFTPVLDLD YGCSRVVGDR AFHRDPLVVA ALAQSLVSGM ADAGMGCVGK 190 200 210 220 230 240 HFPGHGYAEA DSHVEIPVDE REFDAIWTED IAPYRHRLGR QLAGVMPAHV IYPRVDPNPA 250 260 270 280 290 300 GFSRFWLQDI LRGRVGFGGV IFSDDLTMEG ATVVGDILAR ARAAFGAGCD VVLVCNRPDL 310 320 330 340 350 AVDLLDRWAP DIAPESRARI EALRSRPQAA DPFALELHPV YRQARDVVAG LVEDTA

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI09283.1.
RefSeq	YP_160184.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5P081.
Protein family/group databases
CAZy	GH3. Glycoside Hydrolase Family 3.
Genome annotation databases
GeneID	3182301.
GenomeReviews	Gene locus AZOSEA31580 in contig CR555306_GR.
KEGG	eba:ebA5547.
NMPDR	fig|76114.4.peg.3162.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5P081.
OMA	NIDSPRQ.
Enzyme and pathway databases
BioCyc	ASP76114:EBA5547-MON.
Family and domain databases
HAMAP	MF_00364. [Tree]
InterPro	IPR019800. Glyco_hydro_3_AS. IPR001764. Glyco_hydro_3_N. [Graphical view]
Gene3D	G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
Pfam	PF00933. Glyco_hydro_3. 1 hit. [Graphical view]
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGZ_AZOSE

Accession

Primary (citable) accession number: Q5P081

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)