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Q5NZH8

- GLND_AROAE

UniProt

Q5NZH8 - GLND_AROAE

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciAARO76114:GJTA-3463-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:AZOSEA34110
ORF Names:ebA5982
OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Taxonomic identifieri76114 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
ProteomesiUP000006552: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 862862Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192715Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi76114.ebA5982.

Structurei

3D structure databases

ProteinModelPortaliQ5NZH8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini448 – 553106HDUniRule annotationAdd
BLAST
Domaini686 – 76580ACT 1UniRule annotationAdd
BLAST
Domaini794 – 86269ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 328328UridylyltransferaseAdd
BLAST
Regioni329 – 685357Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiLYCLWDM.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5NZH8-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSTAAIPTDA AVQAAIAEAR ARLADGSMRI RMAYEGHPAT STVLKGRAHL
60 70 80 90 100
VDETIHRLWR ACAMPADVAL LAVGGYGRGE LFPCSDVDLM VLLPDTADDA
110 120 130 140 150
MQARLSVLLG ALWDVGLEIG HSARTVAEAI DAAEQDITVQ TNLLESRLLE
160 170 180 190 200
GNRPLFEEFC RRYRALLDVR VFFKAKQLEQ EKRYARYNDT PYALEPNCKE
210 220 230 240 250
SPGGLRDLQM LGWIARAAGL GRNWRDLARR RLITGAEARD LRSIERFLQH
260 270 280 290 300
VRIRLHYLTG RSEDRLLFDY QERLASALGI EATAAKRASE VFMQRYYVNA
310 320 330 340 350
KKVTQTNTIL LQNYGVEIFP RRAGAAIVIN ERFQAVRELL DMREDDTFAR
360 370 380 390 400
HPSALLECFL ILQQRSELKG MTARTLRALW LNRKRINAAF RADPHNRELF
410 420 430 440 450
VAILQQKRGI VHEFRRMNQY GILSGYLPSW RRIVGQMQHD LFHVYTVDQH
460 470 480 490 500
IMMVLRNMRR FTMGEHAHEY PLMAQLIMAF DRHWLLYVAA LFHDIAKGRG
510 520 530 540 550
GDHSKLGTID AREFCEHHHL AREDADLVVW LVEHHLTMSH VAQKEDTSDP
560 570 580 590 600
AVIGRFADTV GTERRLTALY LLTHADIRGT SPKVWNGWKG KLLEDLFFAT
610 620 630 640 650
RRLLRGATPQ EALGLDDRQE NARALLRYHG LRPGVEDALW AQLDAVYFMR
660 670 680 690 700
HSAEEIAWHS RTLYYRPDAL EPVVKARVSD ADQGVQVMVF TRDQKDLFVR
710 720 730 740 750
LTGFFGRLGF SILDAKVHTT RHGYALDSFM LQDPGNAEHY RDVITLIEHE
760 770 780 790 800
LTERLKKSAP PDRPSAGRLS RQVKHFPITP RVSILPDESG RHYILSLTAA
810 820 830 840 850
DRRGLLFAVA EVLAQNGIVL HTAKIATLGE RVEDTFLLSG NGLSQDARVV
860
KIERELLQRL HI
Length:862
Mass (Da):98,195
Last modified:January 4, 2005 - v1
Checksum:iFA31DCA7E434257E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA. Translation: CAI09536.1.
RefSeqiYP_160437.1. NC_006513.1.

Genome annotation databases

EnsemblBacteriaiCAI09536; CAI09536; ebA5982.
GeneIDi3179129.
KEGGieba:ebA5982.
PATRICi20973043. VBIAroAro98752_3439.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA. Translation: CAI09536.1 .
RefSeqi YP_160437.1. NC_006513.1.

3D structure databases

ProteinModelPortali Q5NZH8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 76114.ebA5982.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI09536 ; CAI09536 ; ebA5982 .
GeneIDi 3179129.
KEGGi eba:ebA5982.
PATRICi 20973043. VBIAroAro98752_3439.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi LYCLWDM.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci AARO76114:GJTA-3463-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
    Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
    Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: EbN1.

Entry informationi

Entry nameiGLND_AROAE
AccessioniPrimary (citable) accession number: Q5NZH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: January 4, 2005
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3