Reviewed, UniProtKB/Swiss-Prot Q5NZG9 (DXR_AZOSE)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose 5-phosphate reductoisomerase
      Short name=DXP reductoisomerase
    EC=1.1.1.267
Alternative name(s):
    1-deoxyxylulose-5-phosphate reductoisomerase
    2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	AZOSEA34200
ORF Names:	ebA5994

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00183
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity. HAMAP MF_00183
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_0000163599
Regions
	Nucleotide binding	15 – 44	30	NADP By similarity
Sites
	Metal binding	158	1	Divalent metal cation By similarity
	Metal binding	160	1	Divalent metal cation By similarity
	Metal binding	234	1	Divalent metal cation By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	160	1	Substrate By similarity
	Binding site	189	1	Substrate By similarity
	Binding site	212	1	Substrate By similarity
	Binding site	234	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NZG9-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 89F811F00980A5E4	FASTA	403	43,140
	10 20 30 40 50 60 MSDPTPPRLQ RVTVLGATGS IGMSTLDVLA RHPDRFEAFA LTAQIQVERL FELCLRFSPR 70 80 90 100 110 120 FAVLVDSAAA SDLRQRLKAA GSATEVLAGP GALVDVAAHP DSDAVMAAIV GAAGLAPALA 130 140 150 160 170 180 AARAGKRVLL ANKEALVLSG RLFMQAVVES GAELLPIDSE HNAVFQALPQ GYARDPQRCG 190 200 210 220 230 240 VRRVLLTASG GPFRERSIES LADVTPDEAC AHPNWVMGRK ISVDSATMMN KGLEVIEAHW 250 260 270 280 290 300 LFAVPPEAIE VVVHPQSVIH SMVEYADGSV LAQLGNPDMR TPIAHALAYP ERIDAGVRPL 310 320 330 340 350 360 DLFEIGRLNF ERPDFVRFPC LALAYDALRE GGAAAAVLNA ANEEAVAAFL ERRVGFTRIP 370 380 390 400 DIIAATLERA RDLSVDCIEA ILDADARARE VARSEILARQ TTP

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI09545.1.
RefSeq	YP_160446.1.
3D structure databases
SMR	Q5NZG9. Positions 10-399.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5NZG9.
Genome annotation databases
GeneID	3179978.
GenomeReviews	Gene locus AZOSEA34200 in contig CR555306_GR.
KEGG	eba:ebA5994.
NMPDR	fig|76114.4.peg.3424.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0743.
HOGENOM	HBG430762.
OMA	IHSMVEY.
Enzyme and pathway databases
BioCyc	ASP76114:EBA5994-MONOMER.
Family and domain databases
HAMAP	MF_00183. DXP_reductoisom. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_AZOSE

Accession

Primary (citable) accession number: Q5NZG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	January 4, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)