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Q5NZF8 (BIOD_AROAE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3
Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase
Gene names
Name:bioD
Ordered Locus Names:AZOSEA34310
ORF Names:ebA6011
OrganismAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]
Taxonomic identifier76114 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length225 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336

Catalytic activity

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336

Cofactor

Magnesium By similarity. HAMAP MF_00336

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336

Subcellular location

Cytoplasm By similarity HAMAP MF_00336.

Sequence similarities

Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

dethiobiotin synthase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 225225ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336
PRO_0000302474

Regions

Nucleotide binding118 – 1214ATP By similarity
Nucleotide binding178 – 1792ATP By similarity
Nucleotide binding207 – 2093ATP By similarity

Sites

Metal binding151Magnesium 1 By similarity
Metal binding191Magnesium 2 By similarity
Metal binding571Magnesium 2 By similarity
Metal binding1181Magnesium 2 By similarity
Binding site571ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5NZF8 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: 3787C0C193350982

FASTA22523,820
        10         20         30         40         50         60 
MTEPRAWFLA GTDTEIGKTF VACALLHAAR RDGRSALGMK PVAAGAEWIA GEWLNEDAAQ 

        70         80         90        100        110        120 
LRAASSFDPG LDRLNPYCLK TPVAPHIAAA EEGVEIDPAR IRAAFESLRA QADVVIVEGV 

       130        140        150        160        170        180 
GGFRVPLGDR YDTADLAREL GLPVILVVGM RLGCISHALL TVEAIAARGL TLAGWIANRI 

       190        200        210        220 
DPAMLRADEN LEALRRRIDA PLLGVVPHVA GADPAQVAAS LRLPG

« Hide

References

[1]"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EbN1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CR555306 Genomic DNA. Translation: CAI09556.1.
RefSeqYP_160457.1. NC_006513.1.

3D structure databases

HSSPHSSP built from PDB template 1DAI based on UniProtKB P13000.
ProteinModelPortalQ5NZF8.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5NZF8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3180732.
GenomeReviewsGene locus AZOSEA34310 in contig CR555306_GR.
KEGGeba:ebA6011.
NMPDRfig|76114.4.peg.3472.
PATRIC20973083. VBIAroAro98752_3459.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0132.
HOGENOMHBG650065.
OMAANCVDPN.
ProtClustDBCLSK767011.

Enzyme and pathway databases

BioCycASP76114:EBA6011-MONOMER.

Family and domain databases

HAMAPMF_00336. BioD.
[Tree]
InterProIPR002586. CbiA_P_synth.
IPR004472. DTB_synth_BioD.
[Graphical view]
KOK01935.
PfamPF01656. CbiA. 1 hit.
[Graphical view]
PIRSFPIRSF006755. DTB_synth. 1 hit.
TIGRFAMsTIGR00347. BioD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOD_AROAE
AccessionPrimary (citable) accession number: Q5NZF8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: January 4, 2005
Last modified: January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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