Q5NZ93 (GCSP_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine dehydrogenase [decarboxylating]
EC=1.4.4.2

Alternative name(s):
Glycine cleavage system P-protein
Glycine decarboxylase

Gene names

Name:	gcvP
Ordered Locus Names:	AZOSEA34960
ORF Names:	ebA6124

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum › Aromatoleum aromaticum

Protein attributes

Sequence length	972 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity. HAMAP-Rule MF_00711
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. HAMAP-Rule MF_00711
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvP family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Molecular_function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 972	972	Glycine dehydrogenase [decarboxylating] HAMAP-Rule MF_00711		PRO_0000227090
Amino acid modifications
	Modified residue	713	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NZ93 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 8B37141C4012F18B	FASTA	972	103,927
	10 20 30 40 50 60 MPNASPASPL NAPLAELEQR DAFIGRHVGP NATEIATMLA AVGAPDLDTL IDQTVPPAIR 70 80 90 100 110 120 LAAPLPLEGP KPEHEALADL RAIAAKNVIR KSLIGMGYYG THTPAVILRN VMENPGWYTA 130 140 150 160 170 180 YTPYQAEIAQ GRLEALLNYQ QMVIDLTGLE LANASLLDEA TAAAEAMTMA RRVAKSKSNV 190 200 210 220 230 240 FYVDEACFPQ TIDVLRTRAG LFGFELKFGP AHDAANADAF GALLQYPNER GEIIDLSGTV 250 260 270 280 290 300 AALKAKGAVV AIASDLMALV LLKSPGTMGA DIALGSAQRF GVPMGFGGPH AAFFATREAN 310 320 330 340 350 360 VRAMPGRIIG VSKDARGKTA LRMTLQTREQ HIRREKANSN ICTSQVLLAN MSGFYAVYHG 370 380 390 400 410 420 PQGLRTIAAR IHRLAAILAQ GLRDAGFNVP AGAFFDTLQV DTGARTAELL AACDAAGFNL 430 440 450 460 470 480 RPVSDTVLGL SVDETTTGDD VATLLRLFGA SGELAALDAK VGAAGGAIPA ALLRDDAILT 490 500 510 520 530 540 HPVFNTHHTE HEMLRYLKKL QNRDLALDHS MISLGSCTMK LNATSEMIPI TWAEFANLHP 550 560 570 580 590 600 FAPREQVRGY LEMIDGLAGY LKAVTGFAAI SMQPNSGAQG EYAGLVAIRR YHDSRGDTHR 610 620 630 640 650 660 RVCLIPKSAH GTNPASAQMC GMDVVVVACD ERGNVDLADL EAKVAQHADR LAALMITYPS 670 680 690 700 710 720 THGVFEESIR EICASVHRHG GQVYMDGANL NAQVGLTSPA TIGADVSHMN LHKTFCIPHG 730 740 750 760 770 780 GGGPGMGPIG LAAHLAPFMA DHVVAATGDE TRPNKGQGAV SAAPFGSASI LPISWMYIAM 790 800 810 820 830 840 MGDTGLKLAT EVAILNANYV ANRLAEHYPV LYTGSQGRVA HECILDIRPI KANTGISEVD 850 860 870 880 890 900 IAKRLMDYGF HAPTMSFPVA GTIMIEPTES EDLGELDRFI AAMITIRNEI REVENGAWPT 910 920 930 940 950 960 DDNPLKNAPH TQADFIAADG AQWSRPYSRE QAVFPLPWVA ENKFWPSVNR IDDVYGDRNL 970 FCACVPIEDY AS

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI09621.1.
RefSeq	YP_160522.1. NC_006513.1.
3D structure databases
ProteinModelPortal	Q5NZ93.
ModBase	Search...
Protein-protein interaction databases
STRING	76114.ebA6124.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAI09621; CAI09621; ebA6124.
GeneID	3179225.
KEGG	eba:ebA6124.
PATRIC	20973201. VBIAroAro98752_3518.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1003.
HOGENOM	HOG000239369.
KO	K00281.
OMA	FTESEMI.
ProtClustDB	PRK05367.
Enzyme and pathway databases
BioCyc	AARO76114:GJTA-3548-MONOMER.
Family and domain databases
Gene3D	3.40.640.10. 2 hits.
HAMAP	MF_00711. GcvP.
InterPro	IPR020580. GDC-P_N. IPR020581. GDC_P. IPR003437. GDC_P_homo. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
PANTHER	PTHR11773. PTHR11773. 1 hit.
Pfam	PF02347. GDC-P. 2 hits. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 2 hits.
TIGRFAMs	TIGR00461. gcvP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCSP_AROAE

Accession

Primary (citable) accession number: Q5NZ93

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program