Reviewed, UniProtKB/Swiss-Prot Q5NZ70 (KDSA_AZOSE)

Names and origin

Protein names

Recommended name:
    2-dehydro-3-deoxyphosphooctonate aldolase
    EC=2.5.1.55
Alternative name(s):
    Phospho-2-dehydro-3-deoxyoctonate aldolase
    3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    KDO-8-phosphate synthetase
      Short name=KDO 8-P synthase
      Short name=KDOPS

Gene names

Name:	kdsA
Ordered Locus Names:	AZOSEA35190
ORF Names:	ebA6160

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the kdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_0000304431

Sequences

	Sequence		Length	Mass (Da)
	Q5NZ70-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 4AC1141A41553710	FASTA	279	29,525
	10 20 30 40 50 60 MKLCGFEAGL DKPFFLIAGP CVIESRDMAF ETAGALKEIC VELGIPFIYK SSYDKANRSS 70 80 90 100 110 120 GKSYRGMGME KGLEILADVK KQLGVPVLTD VHAIDEIPAV AAAVDVLQTP AFLCRQTDFI 130 140 150 160 170 180 HAVAASGRPV NIKKGQFLAP GDMKNVVDKA REANGGADTI MVCERGASFG YNNLVSDMRS 190 200 210 220 230 240 LAIMRETGCP VVFDATHSVQ LPGGQGTASG GQREFVPVLA RAAVAVGIAG LFMESHPDPA 250 260 270 KALSDGPNAW PLPKMKALLA TLKEIDALVK AHGFMEMAG

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI09644.1.
RefSeq	YP_160545.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5NZ70.
Genome annotation databases
GeneID	3180663.
GenomeReviews	Gene locus AZOSEA35190 in contig CR555306_GR.
KEGG	eba:ebA6160.
NMPDR	fig|76114.4.peg.3524.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5NZ70.
OMA	EEQCAIV.
Enzyme and pathway databases
BioCyc	ASP76114:EBA6160-MON.
Family and domain databases
HAMAP	MF_00056. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_AZOSE

Accession

Primary (citable) accession number: Q5NZ70

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)