Q5NYA9 (MDH_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	AZOSEA38300
ORF Names:	ebA6695

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum › Aromatoleum aromaticum

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01517
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01517
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Malate dehydrogenase HAMAP-Rule MF_01517		PRO_0000113347
Regions
	Nucleotide binding	12 – 18	7	NAD By similarity
	Nucleotide binding	130 – 132	3	NAD By similarity
Sites
	Active site	191	1	Proton acceptor By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	106	1	NAD By similarity
	Binding site	113	1	NAD By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NYA9 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 69EF1CBDC1B3003F	FASTA	329	35,032
	10 20 30 40 50 60 MSKAPVRVAV TGAAGQIGYS LLFRIASGEM LGKDQPVILQ LLDLPQAQKA VKGVMMELED 70 80 90 100 110 120 CAFPLLAGMV ATDDPNVAFK DADYCLLVGA RPRGPGMERA DLLTANGAIF TVQGKAIAEN 130 140 150 160 170 180 ANENVKVLVV GNPCNTNAYI AGAAARKVGR TNPNNYHGML RLDHNRALSQ LAAKTGRPVS 190 200 210 220 230 240 SLKKMVVWGN HSPTMYADYR FCTSNGDSVK ALVNDHAWNN DVFLPTVGKR GAAIIDARGL 250 260 270 280 290 300 SSAASAANAA IDHMHDWALG SDDWVTMGVP SDGSYGIPAG VVFGVPCECK NGDFKIIQGL 310 320 EIDEYSREKI NKTLGELEDE RAAVADMLK

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI09955.1.
RefSeq	YP_160856.1. NC_006513.1.
3D structure databases
ProteinModelPortal	Q5NYA9.
SMR	Q5NYA9. Positions 3-328.
ModBase	Search...
Protein-protein interaction databases
STRING	76114.ebA6695.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAI09955; CAI09955; ebA6695.
GeneID	3181211.
KEGG	eba:ebA6695.
PATRIC	20973871. VBIAroAro98752_3853.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000220953.
KO	K00024.
OMA	NCLIASK.
ProtClustDB	PRK05442.
Enzyme and pathway databases
BioCyc	AARO76114:GJTA-3882-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_01517. Malate_dehydrog_2.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR010945. Malate_DH_type2. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR23382. PTHR23382. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_AROAE

Accession

Primary (citable) accession number: Q5NYA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program