Malate dehydrogenase - Azoarcus sp. (strain EbN1)

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Reviewed, UniProtKB/Swiss-Prot Q5NYA9 (MDH_AZOSE)

Last modified June 16, 2009. Version 35. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	AZOSEA38300
ORF Names:	ebA6695

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD⁺ = oxaloacetate + NADH. HAMAP MF_01517
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

329

Malate dehydrogenase HAMAP MF_01517

PRO_0000113347

Regions

Nucleotide binding

7

NAD By similarity

Nucleotide binding

3

NAD By similarity

Sites

Active site

1

Proton acceptor By similarity

Binding site

1

Substrate By similarity

Binding site

1

Substrate By similarity

Binding site

1

NAD By similarity

Binding site

1

NAD By similarity

Binding site

1

Substrate By similarity

Binding site

1

Substrate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q5NYA9-1 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: 69EF1CBDC1B3003F
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329

35,032

        10         20         30         40         50         60 
MSKAPVRVAV TGAAGQIGYS LLFRIASGEM LGKDQPVILQ LLDLPQAQKA VKGVMMELED 

        70         80         90        100        110        120 
CAFPLLAGMV ATDDPNVAFK DADYCLLVGA RPRGPGMERA DLLTANGAIF TVQGKAIAEN 

       130        140        150        160        170        180 
ANENVKVLVV GNPCNTNAYI AGAAARKVGR TNPNNYHGML RLDHNRALSQ LAAKTGRPVS 

       190        200        210        220        230        240 
SLKKMVVWGN HSPTMYADYR FCTSNGDSVK ALVNDHAWNN DVFLPTVGKR GAAIIDARGL 

       250        260        270        280        290        300 
SSAASAANAA IDHMHDWALG SDDWVTMGVP SDGSYGIPAG VVFGVPCECK NGDFKIIQGL 

       310        320 
EIDEYSREKI NKTLGELEDE RAAVADMLK

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
	CR555306 Genomic DNA. Translation: CAI09955.1.
RefSeq	YP_160856.1.
3D structure databases
SMR	Q5NYA9. Positions 3-328.
ModBase	Search...
Genome annotation databases
GeneID	3181211.
GenomeReviews	Gene locus AZOSEA38300 in contig CR555306_GR.
KEGG	eba:ebA6695.
NMPDR	fig\|76114.4.peg.3846.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5NYA9.
OMA	Q5NYA9. MIIWGNH.
Enzyme and pathway databases
BioCyc	ASP76114:EBA6695-MON.
Family and domain databases
HAMAP	MF_01517. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010945. Malate_DH_SF1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
ProDom	PD003052. Mal_dehydrog. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_AZOSE

Accession

Primary (citable) accession number: Q5NYA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	January 4, 2005
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents