Q5NY71 (ILVD3_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 3
Short name=DAD 3
EC=4.2.1.9

Gene names

Name:	ilvD3
Ordered Locus Names:	AZOSEA38680
ORF Names:	ebA6761

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 567	567	Dihydroxy-acid dehydratase 3 HAMAP MF_00012		PRO_0000225366
Sites
	Metal binding	130	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	202	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5NY71 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 8126A43B33C7EF2A	FASTA	567	59,892
	10 20 30 40 50 60 MSDPTRPARI DERSRNVTEG VMRAPNRSMY YAMGYRETDF SKPMVGVASA HSTITPCNSG 70 80 90 100 110 120 LQPLADTVVA ALKEAGANPQ LFGTPTVSDG IGMGTEGMKY SLVSREVIAD SIETCVNGLW 130 140 150 160 170 180 QDGVVVIGGC DKNMPGGMMA LVRTNVPGIY VYGGTIKPGH YQGRDLNIIS VFEAVGEFTA 190 200 210 220 230 240 GRLDPVDFKE IEKRACPGSG SCGGMYTANT MSAAFEALGM SLPYSSTMAN EDAEKLASAA 250 260 270 280 290 300 ESARVLVEAI KRGLRPRDIV TREAIENAVS VIMATGGSTN AVLHFLAIAH AAEVPWTIDD 310 320 330 340 350 360 FECIRRRVPV IVDMKPSGHY LATDLHQAGG IPQVMKLLLE AGLLHGECVT ITGRTIAEVL 370 380 390 400 410 420 ENVPAAPRAD QGVIRTLSDP LYAEGHLAIL RGNLSPEGCV AKISGLKNPA ITGPARVFDS 430 440 450 460 470 480 EDDAMGAIMA RRIVEGDVVV IRYEGPKGGP GMREMLAPTS ALVGQGLGES VGLITDGRFS 490 500 510 520 530 540 GGTWGMVVGH VSPEAFVGGP IALVRDGDSV TIDAHRQLVQ LNVGDEELAR RAADWTPPSP 550 560 RYTRGVLAKF AKLTSSASKG AVTDLDL

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI09993.1.
RefSeq	YP_160894.1. NC_006513.1.
3D structure databases
ProteinModelPortal	Q5NY71.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5NY71.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3183070.
GenomeReviews	Gene locus AZOSEA38680 in contig CR555306_GR.
KEGG	eba:ebA6761.
NMPDR	fig|76114.4.peg.3882.
PATRIC	20973941. VBIAroAro98752_3888.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	IAMANAI.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	ASP76114:EBA6761-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD3_AROAE

Accession

Primary (citable) accession number: Q5NY71

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program