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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei246 – 2461ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAARO76114:GJTA-3967-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:AZOSEA39140
ORF Names:ebA6855
OrganismiAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1))
Taxonomic identifieri76114 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAromatoleum
ProteomesiUP000006552 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465Glutamate--tRNA ligasePRO_0000119497Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi76114.ebA6855.

Structurei

3D structure databases

ProteinModelPortaliQ5NY25.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionAdd
BLAST
Motifi243 – 2475"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiNSSPSCF.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5NY25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNVRTRFA PSPTGYLHIG GARTALFSWA FARRHGGTFI LRIEDTDVAR
60 70 80 90 100
STPAAVQAIL DGMSWLGLDA DEGPFYQMQR MDRYKAVIRE MLAAGSAYHC
110 120 130 140 150
YASTEELDRM REEQRARGEK PRYDGRWRPE PGKVLPAMPA GVEPVVRFRN
160 170 180 190 200
PVEGVVAWDD LVKGRIEIAN AELDDLVIAR ADGTPTYNFC VVVDDCDMGI
210 220 230 240 250
THVIRGDDHV NNTPRQINIL RALGAEVPLY AHLSMILGDD GTKLSKRHGA
260 270 280 290 300
VSVMQYFDDG YLPEAVINYL ARLGWSHGDD EIFTREQFVT WFDLDHITPS
310 320 330 340 350
AAQFNTEKLN WLNAHYIKHS DDARLAADVA SRLARRGVDP EAGPRLESVV
360 370 380 390 400
ALYKERVANL NELADAAELY CVAVHPSDEL LAQHLTDAGR AALASLKARL
410 420 430 440 450
ADVAWEKPGL NQAIKDTMAE HSLKMPQVAI PLRVATLGVA QTPAIDAVLE
460
VLGRERVLMR IGRYI
Length:465
Mass (Da):51,617
Last modified:January 4, 2005 - v1
Checksum:iC74703DED6089AAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA. Translation: CAI10039.1.
RefSeqiWP_011239684.1. NC_006513.1.

Genome annotation databases

EnsemblBacteriaiCAI10039; CAI10039; ebA6855.
KEGGieba:ebA6855.
PATRICi20974043. VBIAroAro98752_3938.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR555306 Genomic DNA. Translation: CAI10039.1.
RefSeqiWP_011239684.1. NC_006513.1.

3D structure databases

ProteinModelPortaliQ5NY25.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi76114.ebA6855.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI10039; CAI10039; ebA6855.
KEGGieba:ebA6855.
PATRICi20974043. VBIAroAro98752_3938.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiNSSPSCF.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciAARO76114:GJTA-3967-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
    Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
    Arch. Microbiol. 183:27-36(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: EbN1.

Entry informationi

Entry nameiSYE_AROAE
AccessioniPrimary (citable) accession number: Q5NY25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 4, 2005
Last modified: July 22, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.