Q5NXV7 (GLGB_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	AZOSEA39820
ORF Names:	ebA7003

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	636 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 636	636	1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685		PRO_0000188674
Sites
	Active site	309	1	Nucleophile By similarity
	Active site	362	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NXV7 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 7954F21B483A0042	FASTA	636	71,773
	10 20 30 40 50 60 MDATTTHSRL SEHDIYLFRE GSHGRLYDKL GCQLDEAGAH FAVWAPNARA VAVIGSFNAW 70 80 90 100 110 120 RDDAAVLRPR DDGSGIWEGF VAGVAAGDVY KYSIVCQDGR VAEKADPFAQ YAEVPPATGS 130 140 150 160 170 180 RAWRSAHRWA DDAWMAGRAR ANALDAPFSI YELHLGSWRR AQDGALPGYR EIAPQLAAYV 190 200 210 220 230 240 VEWGFTHVEL MPLSEHPFYG SWGYQTTGYF AATARYGTPD DLMFLIDTLH QAGVGVILDW 250 260 270 280 290 300 VPSHFPSDAH GLAEFDGTYL YEHADPRQGF HPEWHSCIFN YGRHEVCAFL LSSALFWLER 310 320 330 340 350 360 FHIDGLRVDA VASMLYLDYG RQHGEWVPNR YGGRENLDAV AFLRRLNEAV YRDHPGVQTI 370 380 390 400 410 420 AEESTAWPMV SRPLYVGGLG FGMKWNMGWM HDSLDYFRHD PLFRKFHHGR ITFSIWYAFH 430 440 450 460 470 480 ENFVLPLSHD EVVYGKGSLI GKMPGDSWQQ FAGLRALFGY MWAHPGKKLL FMGGEFGQRR 490 500 510 520 530 540 EWTHEGELEW WVLDRPEHAG LRHWVGDLNR LYRERAALHE LDFDEAGFQW IDSDDSENSV 550 560 570 580 590 600 LSFLRKSRNG ATVLVICNFT PVTRPNYTLG VPRAGFWREA LNSDATLYGG SGAGNLGGVE 610 620 630 TVPVPAHGHY QSLTLTLPPL AVLFLIPEVD DARPDS

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI10107.1.
RefSeq	YP_161008.2. NC_006513.1.
3D structure databases
ProteinModelPortal	Q5NXV7.
SMR	Q5NXV7. Positions 22-630.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5NXV7.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3179164.
GenomeReviews	Gene locus AZOSEA39820 in contig CR555306_GR.
KEGG	eba:ebA7003.
NMPDR	fig|76114.4.peg.3998.
PATRIC	20974195. VBIAroAro98752_4012.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	RVYHQNG.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	ASP76114:EBA7003-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_AROAE

Accession

Primary (citable) accession number: Q5NXV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program