Q5NXU8 (SYS_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine--tRNA ligase
EC=6.1.1.11

Alternative name(s):
Seryl-tRNA synthetase
Short name=SerRS
Seryl-tRNA(Ser/Sec) synthetase

Gene names

Name:	serS
Ordered Locus Names:	AZOSEA39910
ORF Names:	ebA7034

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity. HAMAP MF_00176
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). HAMAP MF_00176 ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). HAMAP MF_00176
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. HAMAP MF_00176
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00176.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity. HAMAP MF_00176
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	seryl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Serine--tRNA ligase HAMAP MF_00176		PRO_0000121996
Regions
	Nucleotide binding	263 – 265	3	ATP By similarity
	Nucleotide binding	350 – 353	4	ATP By similarity
	Region	232 – 234	3	Serine binding By similarity
Sites
	Binding site	286	1	Serine By similarity
	Binding site	385	1	Serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NXU8 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 96423CFB1657143F	FASTA	427	47,052
	10 20 30 40 50 60 MLDIQLLRTQ IDAVAAALAA RGANFDAAAF QSMENERKTL QTRTQDLQAR RNTLSKQIGV 70 80 90 100 110 120 LKSRGEDASA AMAEVGGIGD ELKANEQALA TLLERINAFV AGLPNLPHDS VPPGRDESAN 130 140 150 160 170 180 VEIARWGTPR EFDFEVSDHV DIGSGLGGLD FETAAKISGS RFALMRDGLA RLHRALAQFM 190 200 210 220 230 240 LDVHTREHGY TEVHVPYLVN PDSMFGTGQL PKFEAELFSV MKDDGRFYLI PTAEVPITNI 250 260 270 280 290 300 VRNELLAHDV LPLKFVGHTP CFRSEAGSYG RDTRGMIRQH QFDKVELVRI EHPDASWAAL 310 320 330 340 350 360 EELTGHAEAI LRKLELPYRK VVLCTGDMGF SAAKTYDLEV WLPAQKTYRE ISSCSCTGAF 370 380 390 400 410 420 QARRMQARFR NTQGKNELVH TLNGSGLAVG RTLVAVLENY QQADGSVVVP KVLVPWMGGI EVLEPRG

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI10116.1.
RefSeq	YP_161017.1. NC_006513.1.
3D structure databases
ProteinModelPortal	Q5NXU8.
SMR	Q5NXU8. Positions 1-426.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5NXU8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3182502.
GenomeReviews	Gene locus AZOSEA39910 in contig CR555306_GR.
KEGG	eba:ebA7034.
NMPDR	fig|76114.4.peg.4007.
PATRIC	20974215. VBIAroAro98752_4021.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0172.
HOGENOM	HBG629391.
OMA	PKFADDM.
ProtClustDB	PRK05431.
Enzyme and pathway databases
BioCyc	ASP76114:EBA7034-MONOMER.
Family and domain databases
HAMAP	MF_00176. Ser_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR002317. Ser-tRNA-synth_IIa. IPR015866. Ser-tRNA-synth_IIa_N. IPR010978. tRNA-bd_arm. [Graphical view]
Gene3D	G3DSA:1.10.287.40. Ser-tRNA-synth_IIa_N. 1 hit.
KO	K01875.
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00414. SerS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYS_AROAE

Accession

Primary (citable) accession number: Q5NXU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program