Reviewed, UniProtKB/Swiss-Prot Q5NXR0 (PUR5_AZOSE)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
Alternative name(s):
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    AIR synthase

Gene names

Name:	purM
Ordered Locus Names:	AZOSEA40290
ORF Names:	ebA7104

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741		PRO_0000258327

Sequences

	Sequence		Length	Mass (Da)
	Q5NXR0-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: A0D2757D48C1610F	FASTA	348	36,666
	10 20 30 40 50 60 MSSPKPSLSY RDAGVDIDAG DALVDRIKPL AKRTMRPEVL GGIGGFGALF ELSKKYREPV 70 80 90 100 110 120 LVSGTDGVGT KLKLAFQLNR HDTVGQDLVA MSVNDILVQG AEPLFFLDYF ACGKLDVDTA 130 140 150 160 170 180 AAVVSGIARG CELSGCALIG GETAEMHGMY PDGEYDLAGF AVGAVEKSEI IDGSRIVPGD 190 200 210 220 230 240 VVLGLASSGA HSNGYSLIRK IIDLAKPDLD ADFHGRPLRD VILEPTRLYV KPMLGLMQAI 250 260 270 280 290 300 PGVVKGMAHI TGGGLLENVP RILADGLAAR LDVSSWTLPP LFQWLRDAGN VDAQEMYRVF 310 320 330 340 NCGVGMVVIV SAAQAGAAVQ NLEAAGEIVY RLGRIESRAE GAAQTTVG

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI10154.1.
RefSeq	YP_161055.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5NXR0.
Genome annotation databases
GeneID	3180766.
GenomeReviews	Gene locus AZOSEA40290 in contig CR555306_GR.
KEGG	eba:ebA7104.
NMPDR	fig|76114.4.peg.4045.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5NXR0.
OMA	VHGLAHI.
Enzyme and pathway databases
BioCyc	ASP76114:EBA7104-MON.
Family and domain databases
HAMAP	MF_00741. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_AZOSE

Accession

Primary (citable) accession number: Q5NXR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)