Q5NXQ3 (PANB_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase
EC=2.1.2.11

Alternative name(s):
Ketopantoate hydroxymethyltransferase
Short name=KPHMT

Gene names

Name:	panB
Ordered Locus Names:	AZOSEA40360
ORF Names:	ebA7119

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity. HAMAP MF_00156
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00156
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity. HAMAP MF_00156
Subcellular location	Cytoplasm Potential HAMAP MF_00156.
Sequence similarities	Belongs to the PanB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	3-methyl-2-oxobutanoate hydroxymethyltransferase HAMAP MF_00156		PRO_0000297219
Regions
	Region	51 – 52	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	188	1	Proton acceptor By similarity
	Metal binding	51	1	Magnesium By similarity
	Metal binding	90	1	Magnesium By similarity
	Metal binding	121	1	Magnesium By similarity
	Binding site	90	1	Alpha-ketoisovalerate By similarity
	Binding site	119	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NXQ3 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: EEBD7950BD7C134D	FASTA	271	28,881
	10 20 30 40 50 60 MSYLQDDKPV TLFEIGKMRA EGRKISMLTC YDASFASLLE RAGVDILLVG DSLGNVVQGQ 70 80 90 100 110 120 KSTLPVTLEH MIYHTECVVR GSTRPFIVTD MPFGAYHESP SQAMHNAASL LAAGAQMVKL 130 140 150 160 170 180 EGGTFMAETV RFLVERGIPV CAHIGLTPQS VHQLGGYRVQ GRSEAAAAQL KGDALALEQA 190 200 210 220 230 240 GAALMVMEMV PAALAREVTA SLASMATIGI GAGPDCDGQV LVLHDMIGVY PGKKARFVRN 250 260 270 FMTGKTDIDE AVASYVQAVR DGSFPAAEHC Y

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI10161.1.
RefSeq	YP_161062.1. NC_006513.1.
3D structure databases
HSSP	HSSP built from PDB template 1O66 based on UniProtKB Q9JZW6.
ProteinModelPortal	Q5NXQ3.
SMR	Q5NXQ3. Positions 10-271.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5NXQ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3181660.
GenomeReviews	Gene locus AZOSEA40360 in contig CR555306_GR.
KEGG	eba:ebA7119.
NMPDR	fig|76114.4.peg.4052.
PATRIC	20974317. VBIAroAro98752_4072.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0413.
HOGENOM	HBG299908.
OMA	YDATFAH.
ProtClustDB	PRK00311.
Enzyme and pathway databases
BioCyc	ASP76114:EBA7119-MONOMER.
Family and domain databases
HAMAP	MF_00156. PanB. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K00606.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR00222. PanB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB_AROAE

Accession

Primary (citable) accession number: Q5NXQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program