Reviewed, UniProtKB/Swiss-Prot Q5NXP4 (ILVC_AZOSE)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	AZOSEA40450
ORF Names:	ebA7134

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Ketol-acid reductoisomerase		PRO_0000226154
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5NXP4-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: EFAD5A86A348B1C5	FASTA	338	36,966
	10 20 30 40 50 60 MKVYYDKDAD LSLIKGKQIT IVGYGSQGHA HAQNLKDSGC KVTVGLRKSG ASWKKAEAAG 70 80 90 100 110 120 LDVREVAEAV SNADIVMILL PDENIPGVYY NDVEPNIKQG ATLAFAHGFN VHYNQVVPRD 130 140 150 160 170 180 DLDVIMVAPK GPGHTVRSEY LKGGGVPSLI AVHQDRSGKA KDIALSYAAA NGGTKGGVIE 190 200 210 220 230 240 TNFREETETD LFGEQAVLCG GAVELVKMGF ETLTEAGYAP EMAYFECLHE LKLIVDLMYE 250 260 270 280 290 300 GGIANMNYSI SNNAEYGEYV TGPEVINEQS REAMRAALKR IQTGEYAKMF IQEGRTNYPS 310 320 330 MTARRRLNAA HPIEQVGGQL RDMMPWIKKN KLVDQSKN

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI10170.1.
RefSeq	YP_161071.1.
3D structure databases
SMR	Q5NXP4. Positions 1-327.
ModBase	Search...
Genome annotation databases
GeneID	3181730.
GenomeReviews	Gene locus AZOSEA40450 in contig CR555306_GR.
KEGG	eba:ebA7134.
NMPDR	fig|76114.4.peg.4061.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5NXP4.
Enzyme and pathway databases
BioCyc	ASP76114:EBA7134-MON.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_AZOSE

Accession

Primary (citable) accession number: Q5NXP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	January 4, 2005
Last modified:	November 25, 2008
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)