Q5NXP4 (ILVC_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	AZOSEA40450
ORF Names:	ebA7134

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000226154
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5NXP4 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: EFAD5A86A348B1C5	FASTA	338	36,966
	10 20 30 40 50 60 MKVYYDKDAD LSLIKGKQIT IVGYGSQGHA HAQNLKDSGC KVTVGLRKSG ASWKKAEAAG 70 80 90 100 110 120 LDVREVAEAV SNADIVMILL PDENIPGVYY NDVEPNIKQG ATLAFAHGFN VHYNQVVPRD 130 140 150 160 170 180 DLDVIMVAPK GPGHTVRSEY LKGGGVPSLI AVHQDRSGKA KDIALSYAAA NGGTKGGVIE 190 200 210 220 230 240 TNFREETETD LFGEQAVLCG GAVELVKMGF ETLTEAGYAP EMAYFECLHE LKLIVDLMYE 250 260 270 280 290 300 GGIANMNYSI SNNAEYGEYV TGPEVINEQS REAMRAALKR IQTGEYAKMF IQEGRTNYPS 310 320 330 MTARRRLNAA HPIEQVGGQL RDMMPWIKKN KLVDQSKN

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI10170.1.
RefSeq	YP_161071.1. NC_006513.1.
3D structure databases
ProteinModelPortal	Q5NXP4.
SMR	Q5NXP4. Positions 1-327.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5NXP4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3181730.
GenomeReviews	Gene locus AZOSEA40450 in contig CR555306_GR.
KEGG	eba:ebA7134.
NMPDR	fig|76114.4.peg.4061.
PATRIC	20974337. VBIAroAro98752_4082.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0059.
HOGENOM	HBG286525.
OMA	MAYAKGI.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	ASP76114:EBA7134-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_AROAE

Accession

Primary (citable) accession number: Q5NXP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program