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Q5NXM5 (TRMD_AROAE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:AZOSEA40640
ORF Names:ebA7168
OrganismAromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]
Taxonomic identifier76114 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605

Subunit structure

Homodimer By similarity. HAMAP MF_00605

Subcellular location

Cytoplasm Potential HAMAP MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA (guanine-N1-)-methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 263263tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
PRO_0000060318

Regions

Region144 – 1496S-adenosyl-L-methionine binding By similarity

Sites

Binding site1241S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5NXM5 [UniParc].

Last modified January 4, 2005. Version 1.
Checksum: E3BE44CC30999261

FASTA26328,939
        10         20         30         40         50         60 
MNTPAAARRF DVITLFPEMF AALTASGITR RALERGLYEI AFHSPRDFVS DPHRTVDDRP 

        70         80         90        100        110        120 
YGGGPGMVML AEPLEKAIGR AKTAQEQALG AAGRVIYLSP QGRPLDHAKV VEMTALPALT 

       130        140        150        160        170        180 
LLCGRYEGVD QRLIDRCVDE ELSLGDFVLS GGELPAMVLL DAIVRQLPGA LNDADSAQED 

       190        200        210        220        230        240 
SFVDGLLDCP HYTRPEIYAG ERVPQVLLSG NHAAIRRWRL KQALGTTWRR RPDLLQGRTL 

       250        260 
SKEELSLLGE FRHEQEGVEG DIA

« Hide

References

[1]"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1."
Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.
Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EbN1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CR555306 Genomic DNA. Translation: CAI10189.1.
RefSeqYP_161090.1. NC_006513.1.

3D structure databases

ProteinModelPortalQ5NXM5.
SMRQ5NXM5. Positions 9-256.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5NXM5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3181130.
GenomeReviewsGene locus AZOSEA40640 in contig CR555306_GR.
KEGGeba:ebA7168.
NMPDRfig|76114.4.peg.4079.
PATRIC20974377. VBIAroAro98752_4102.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0336.
HOGENOMHBG285805.
OMAVCGRFEG.
ProtClustDBPRK00026.

Enzyme and pathway databases

BioCycASP76114:EBA7168-MONOMER.

Family and domain databases

HAMAPMF_00605. TrmD.
[Tree]
InterProIPR016009. tRNA_m1G_MeTrfase.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
[Graphical view]
Gene3DG3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KOK00554.
PANTHERPTHR10056. PTHR10056. 1 hit.
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMsTIGR00088. TrmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_AROAE
AccessionPrimary (citable) accession number: Q5NXM5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: January 4, 2005
Last modified: January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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