Reviewed, UniProtKB/Swiss-Prot Q5NXM5 (TRMD_AZOSE)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	AZOSEA40640
ORF Names:	ebA7168

Organism

Azoarcus sp. (strain EbN1) (Aromatoleum aromaticum (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060318
Regions
	Region	144 – 149	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	124	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NXM5-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: E3BE44CC30999261	FASTA	263	28,939
	10 20 30 40 50 60 MNTPAAARRF DVITLFPEMF AALTASGITR RALERGLYEI AFHSPRDFVS DPHRTVDDRP 70 80 90 100 110 120 YGGGPGMVML AEPLEKAIGR AKTAQEQALG AAGRVIYLSP QGRPLDHAKV VEMTALPALT 130 140 150 160 170 180 LLCGRYEGVD QRLIDRCVDE ELSLGDFVLS GGELPAMVLL DAIVRQLPGA LNDADSAQED 190 200 210 220 230 240 SFVDGLLDCP HYTRPEIYAG ERVPQVLLSG NHAAIRRWRL KQALGTTWRR RPDLLQGRTL 250 260 SKEELSLLGE FRHEQEGVEG DIA

References

[1]	"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR555306 Genomic DNA. Translation: CAI10189.1.
RefSeq	YP_161090.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5NXM5.
Genome annotation databases
GeneID	3181130.
GenomeReviews	Gene locus AZOSEA40640 in contig CR555306_GR.
KEGG	eba:ebA7168.
NMPDR	fig|76114.4.peg.4079.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5NXM5.
OMA	HRSVDDT.
Enzyme and pathway databases
BioCyc	ASP76114:EBA7168-MON.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_AZOSE

Accession

Primary (citable) accession number: Q5NXM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)