Q5NXM1 (AMPA_AROAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	AZOSEA40680
ORF Names:	ebA7174

Organism

Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) [Complete proteome] [HAMAP]

Taxonomic identifier

76114 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000019880
Sites
	Active site	280	1	Potential
	Active site	354	1	Potential
	Metal binding	268	1	Manganese 2 By similarity
	Metal binding	273	1	Manganese 1 By similarity
	Metal binding	273	1	Manganese 2 By similarity
	Metal binding	291	1	Manganese 2 By similarity
	Metal binding	350	1	Manganese 1 By similarity
	Metal binding	352	1	Manganese 1 By similarity
	Metal binding	352	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NXM1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 332FA8DC26D73770	FASTA	500	52,362
	10 20 30 40 50 60 MEFTIKTGTP EKLKTGSVVV GVFADGQLGD AAAALDKASK GKFAAILGRG DLEDKAGSLL 70 80 90 100 110 120 AIHDLPGSAC ERVLAVSLGK RDEFGDKAWR DALVAIGKAL ASGAASDVAV CLTDTPVPGR 130 140 150 160 170 180 DIDWVLQQLV RAIADGAYRF DATKSKDKGK DSKRRGAGKV VLLTREITAS MEAAIQRGQA 190 200 210 220 230 240 IAEGMALAKD LGNLPGNFCT PSYLADTAVA LGKQYKLKVE VLDRDDMEKL GMGSLLSVAR 250 260 270 280 290 300 GSHQPPRFIV MHYKGGKSKD KPVVLVGKGI TFDSGGISLK PGAEMDEMKF DMCGAASVLG 310 320 330 340 350 360 TFKAIARMAL PINVVGLIPT TENMPGGGAT KPGDVVTSMS GQTIEILNTD AEGRLILCDA 370 380 390 400 410 420 LTYAERFKPA CVVDIATLTG ACVVALGKIP SGLLANDDAL ARELLDCGTV SGDRAWQLPL 430 440 450 460 470 480 WDEYQELLKS NFADMGNIGG RFAGTITAAC FLARFTKAYK WAHLDIAGTA WVSGDAKGAT 490 500 GRPVPLLAEF LIARSQGAPG

References

[1]

"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1." Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R. Arch. Microbiol. 183:27-36(2005) [PubMed: 15551059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EbN1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR555306 Genomic DNA. Translation: CAI10193.1.
RefSeq	YP_161094.1. NC_006513.1.
3D structure databases
HSSP	HSSP built from PDB template 1GYT based on UniProtKB P68767.
ProteinModelPortal	Q5NXM1.
SMR	Q5NXM1. Positions 1-495.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5NXM1.
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3181364.
GenomeReviews	Gene locus AZOSEA40680 in contig CR555306_GR.
KEGG	eba:ebA7174.
NMPDR	fig|76114.4.peg.4083.
PATRIC	20974385. VBIAroAro98752_4106.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0260.
HOGENOM	HBG742580.
OMA	NSGLMST.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	ASP76114:EBA7174-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_AROAE

Accession

Primary (citable) accession number: Q5NXM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program