Reviewed, UniProtKB/Swiss-Prot Q5NVT1 (F262_PONAB)

Names and origin

Protein names

Recommended name:
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Alternative name(s):
    PFK-2/FBPase-2
    6PF-2-K/Fru-2,6-P2ASE heart-type isozyme
Including the following 2 domains:
    1- Recommended name:
            6-phosphofructo-2-kinase
              EC=2.7.1.105
    2- Recommended name:
            Fructose-2,6-bisphosphatase
              EC=3.1.3.46

Gene names

Name:

PFKFB2

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Synthesis and degradation of fructose 2,6-bisphosphate By similarity.
Catalytic activity	Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.
Enzyme regulation	Phosphorylation results in the activation of the kinase activity By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	In the C-terminal section; belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
PTM	Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	fructose 2,6-bisphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructo-2-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose-2,6-bisphosphate 2-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2		PRO_0000345129
Regions
	Nucleotide binding	45 – 52	8	ATP Potential
	Region	1 – 248	248	6-phosphofructo-2-kinase
	Region	249 – 530	282	Fructose-2,6-bisphosphatase
Sites
	Active site	128	1	Potential
	Active site	158	1	Potential
	Active site	257	1	Tele-phosphohistidine intermediate By similarity
	Active site	326	1	Potential
	Active site	391	1	Proton donor By similarity
	Binding site	102	1	Fructose-6-phosphate By similarity
	Binding site	193	1	Fructose-6-phosphate By similarity
Amino acid modifications
	Modified residue	29	1	Phosphoserine; by PKA By similarity
	Modified residue	466	1	Phosphoserine; by PKA By similarity
	Modified residue	468	1	Phosphothreonine By similarity
	Modified residue	475	1	Phosphothreonine; by PKC By similarity
	Modified residue	483	1	Phosphoserine By similarity
	Modified residue	493	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NVT1-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: B279884ABFB8780E	FASTA	530	61,168
	10 20 30 40 50 60 MSGASSSEQN NNSYETKPPN LRMSEKKCSW ASYMTNSPTL IVMIGLPARG KTYVSKKLTR 70 80 90 100 110 120 YLNWIGVPTK VFNLGVYRRE AVKSYKSYDF FRHDNEEAMK IRKQCALVAL EDVKAYLTEE 130 140 150 160 170 180 NGQIAVFDAT NTTRERRDMI LNFAEQNSFK VFFVESVCDD PDVIAANILE VKVSSPDYPE 190 200 210 220 230 240 RNRENVMEDF LKRIECYKVT YRPLDPDNYD KDLSFIKVIN VGQRFLVNRV QDYIQSKIVY 250 260 270 280 290 300 YLMNIHVQPR TIYLCRHGES EFSLLGKIGG DSGLSVRGKQ FAQALRKFLE EQEITDLKVW 310 320 330 340 350 360 TSQLKRTIQT AESLGVPYEQ WKILNEIDAG VCEEMTYAEI EKRYPEEFAL RDQEKYLYRY 370 380 390 400 410 420 PGGESYQDLV QRLEPVIMEL ERQGNVLVIS HQAVMRCLLA YFLDKGADEL PYLRCPLHTI 430 440 450 460 470 480 FKLTPVAYGC KVETIKLNVE AVNTHRDKPT NNFPKNQTPV RMRRNSFTPL SSSNTIRRPR 490 500 510 520 530 NYSVGSRPLK PLSPLRAQDM QEGPTSRRPK SHSGWCTVCF PPALASCPCH

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR925919 mRNA. Translation: CAI29582.1.
RefSeq	NP_001127057.1.
UniGene	Pab.12002
3D structure databases
SMR	Q5NVT1. Positions 38-451.
ModBase	Search...
Genome annotation databases
GeneID	100174086.
Phylogenomic databases
HOVERGEN	Q5NVT1.
Family and domain databases
InterPro	IPR003094. 6Pfruct_kin. IPR013079. 6Phosfructo_kin. IPR016260. Bifunct_6PFK/fruc_bisP_Ptase. IPR013078. PG_mutase. [Graphical view]
PANTHER	PTHR10606. 6Pfruct_kin. 1 hit.
Pfam	PF01591. 6PF2K. 1 hit. PF00300. PGAM. 1 hit. [Graphical view]
PIRSF	PIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTS	PR00991. 6PFRUCTKNASE.
ProtoNet	Search...

Entry information

Entry name

F262_PONAB

Accession

Primary (citable) accession number: Q5NVT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	January 4, 2005
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)