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Protein

Beta-1,4-galactosyltransferase 3

Gene

B4GALT3

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi197ManganeseBy similarity1
Binding sitei226UDP-alpha-D-galactoseBy similarity1
Binding sitei258UDP-alpha-D-galactoseBy similarity1
Metal bindingi291Manganese; via tele nitrogenBy similarity1
Binding sitei303N-acetyl-D-glucosamineBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 3 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 3
Short name:
Beta4Gal-T3
Short name:
b4Gal-T3
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
Including the following 3 domains:
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
Gene namesi
Name:B4GALT3
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
Proteomesi
  • UP000001595 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 10CytoplasmicSequence analysis10
Transmembranei11 – 31Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini32 – 393LumenalSequence analysisAdd BLAST362

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805391 – 393Beta-1,4-galactosyltransferase 3Add BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi57N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi77 ↔ 119By similarity
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi190 ↔ 209By similarity
Glycosylationi337N-linked (GlcNAc...)Sequence analysis1
Glycosylationi385N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000000709.

Structurei

3D structure databases

ProteinModelPortaliQ5NVN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni130 – 134UDP-alpha-D-galactose bindingBy similarity5
Regioni169 – 171UDP-alpha-D-galactose bindingBy similarity3
Regioni196 – 197UDP-alpha-D-galactose bindingBy similarity2
Regioni260 – 263N-acetyl-D-glucosamine bindingBy similarity4
Regioni291 – 293UDP-alpha-D-galactose bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
HOVERGENiHBG058334.
InParanoidiQ5NVN3.
KOiK07968.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5NVN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DQGPTFDYSH
60 70 80 90 100
PRDVYSNLSH LPGAPGGPPA PQGLPYCPER SPLLVGPVSV SFSPVPSLAE
110 120 130 140 150
IVERNPRVEP GGRYRPAGCE PRSRTAIIVP HRAREHHLRL LLYHLHPFLQ
160 170 180 190 200
RQQLAYGIYV IHQAGNGTFN RAKLLNVGVR EALRDEEWDC LFLHDVDLLP
210 220 230 240 250
ENDHNLYVCD PRGPRHVAVA MNKFGYSLPY PQYFGGVSAL TPDQYLKMNG
260 270 280 290 300
FPNEYWGWGG EDDDIATRVR LAGMKISRPP TSVGHYKMVK HRGDKGNEEN
310 320 330 340 350
PHRFDLLVRT QNSWTQDGMN SLTYQLLARE LGPLYTNITA DIGTDPRGPR
360 370 380 390
APSGPRYPPG SSQAFRQEML QRRPPARPGP PPTANHTALR GSH
Length:393
Mass (Da):43,922
Last modified:January 4, 2005 - v1
Checksum:iCA0BF9562D32D1F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR925982 mRNA. Translation: CAI29630.1.
RefSeqiNP_001127681.1. NM_001134209.1.
UniGeneiPab.671.

Genome annotation databases

GeneIDi100174763.
KEGGipon:100174763.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR925982 mRNA. Translation: CAI29630.1.
RefSeqiNP_001127681.1. NM_001134209.1.
UniGeneiPab.671.

3D structure databases

ProteinModelPortaliQ5NVN3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000000709.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100174763.
KEGGipon:100174763.

Organism-specific databases

CTDi8703.

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
HOVERGENiHBG058334.
InParanoidiQ5NVN3.
KOiK07968.

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB4GT3_PONAB
AccessioniPrimary (citable) accession number: Q5NVN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: January 4, 2005
Last modified: November 30, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.