Reviewed, UniProtKB/Swiss-Prot Q5NVF6 (PPAL_PONAB)

Names and origin

Protein names

Recommended name:
    Lysosomal acid phosphatase
      Short name=LAP
    EC=3.1.3.2

Gene names

Name:

ACP2

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Subcellular location	Lysosome membrane; Single-pass membrane protein; Lumenal side By similarity. Lysosome lumen By similarity. Note: The soluble form arises by proteolytic processing of the membrane-bound form By similarity.
Post-translational modification	The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen By similarity.
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Lysosome Membrane
Domain	Signal Transmembrane
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	By similarity
	Chain	31 – 423	393	Lysosomal acid phosphatase		PRO_0000352522
Regions
	Topological domain	31 – 380	350	Lumenal Potential
	Transmembrane	381 – 401	21	Potential
	Topological domain	402 – 423	22	Cytoplasmic Potential
Sites
	Active site	42	1	Nucleophile By similarity
	Active site	287	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	177	1	N-linked (GlcNAc...) Potential
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	267	1	N-linked (GlcNAc...) Potential
	Glycosylation	322	1	N-linked (GlcNAc...) Potential
	Glycosylation	331	1	N-linked (GlcNAc...) Potential
	Disulfide bond	159 ↔ 370		By similarity
	Disulfide bond	212 ↔ 310		By similarity
	Disulfide bond	345 ↔ 349		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NVF6-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 1A2CBFA5D8080E2B	FASTA	423	48,302
	10 20 30 40 50 60 MAGKRSGWSR AALLQLLLGV NLVVMPPTQA RSLRFVTLLY RHGDRSPVKT YPKDPYQEEE 70 80 90 100 110 120 WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNTSYHRQEV YVRSTDFDRT LMSAEANLAG 130 140 150 160 170 180 LFPPNGMQRF NPNISWQPIP VHTVPITEDR LLKFPLGPCP RYEQLQNETR QTPEYQNESS 190 200 210 220 230 240 RNAQFLDMVA NETGLTDLTL ETVWNVYDTL FCEQTHGLRL PPWASPQTMQ RLSRLKDFSF 250 260 270 280 290 300 RFLFGIYQQA EKARLQGGVL LAQIRKNLTL MATTSQLPKL LVYSAHDTTL VALQMALDVY 310 320 330 340 350 360 NGEQAPYASC HIFELYQEDS GNFSVEMYFR NESDKAPWPL SLPGCPHRCP LQDFLRLTEP 370 380 390 400 410 420 VVPKDWQQEC QVASGPADTE VIVALAVCGS ILFLLIVLLL TVLFRMQAQP PGYRHVADGE DHA

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR926080 mRNA. Translation: CAI29707.1.
RefSeq	NP_001127113.1.
UniGene	Pab.18390
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	100174154.
Organism-specific databases
CTD	100174154.
Phylogenomic databases
HOVERGEN	Q5NVF6.
OMA	ELYQEDS.
Family and domain databases
InterPro	IPR000560. Histidine_acid_Pase. [Graphical view]
Pfam	PF00328. Acid_phosphat_A. 1 hit. [Graphical view]
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPAL_PONAB

Accession

Primary (citable) accession number: Q5NVF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	January 4, 2005
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)