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Protein

2-hydroxyisoflavanone dehydratase

Gene

HIDM

Organism
Glycyrrhiza echinata (Licorice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Dehydratase that mediates the biosynthesis of isoflavonoids. Can better use 2,7-dihydroxy-4'-methoxyisoflavanone as substrate. Has also a slight carboxylesterase activity toward p-nitrophenyl butyrate.1 Publication

Catalytic activityi

2,7,4'-trihydroxyisoflavanone = daidzein + H2O.1 Publication
2,4',5,7-tetrahydroxyisoflavanone = genistein + H2O.1 Publication
A carboxylic ester + H2O = an alcohol + a carboxylate.1 Publication

Kineticsi

kcat is 9.8 sec(-1) with 2,7-dihydroxy-4'-methoxyisoflavanone, 0.12 sec(-1) with 2,7,4'-trihydroxyisoflavanone and 0.19 sec(-1) with 2,5,7,4'-tetrahydroxyisoflavanone as substrates, respectively (at pH 7.5 and 30 degrees Celsius).

  1. KM=58 µM for 2,7-dihydroxy-4'-methoxyisoflavanone (at pH 7.5 and 30 degrees Celsius)1 Publication
  2. KM=210 µM for 2,7,4'-trihydroxyisoflavanone (at pH 7.5 and 30 degrees Celsius)1 Publication
  3. KM=304 µM for 2,5,7,4'-tetrahydroxyisoflavanone (at pH 7.5 and 30 degrees Celsius)1 Publication

    Pathwayi: flavonoid biosynthesis

    This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei173By similarity1
    Active sitei272By similarity1
    Active sitei304By similarity1

    GO - Molecular functioni

    • 2-hydroxyisoflavanone dehydratase activity Source: UniProtKB
    • carboxylic ester hydrolase activity Source: UniProtKB

    GO - Biological processi

    • flavonoid biosynthetic process Source: UniProtKB-UniPathway
    • isoflavonoid biosynthetic process Source: UniProtKB
    • isoflavonoid metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Lyase

    Keywords - Biological processi

    Flavonoid biosynthesis

    Enzyme and pathway databases

    BRENDAi4.2.1.105. 2486.
    SABIO-RKQ5NUF4.
    UniPathwayiUPA00154.

    Protein family/group databases

    ESTHERiglyec-q5nuf4. Plant_carboxylesterase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-hydroxyisoflavanone dehydratase (EC:3.1.1.1, EC:4.2.1.105)
    Alternative name(s):
    Carboxylesterase HIDM
    Gene namesi
    Name:HIDM
    OrganismiGlycyrrhiza echinata (Licorice)
    Taxonomic identifieri46348 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeGalegeaeGlycyrrhiza

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004241021 – 3282-hydroxyisoflavanone dehydrataseAdd BLAST328

    Structurei

    3D structure databases

    ProteinModelPortaliQ5NUF4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi85 – 87Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion holeBy similarity3

    Sequence similaritiesi

    Belongs to the 'GDXG' lipolytic enzyme family.Curated

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR013094. AB_hydrolase_3.
    [Graphical view]
    PfamiPF07859. Abhydrolase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5NUF4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MASSTSTTTS KEIDRELPPL LRVYKDGTVE RFLGSSFVPP SPEDPETGVS
    60 70 80 90 100
    TKDIVISENP TISARVYLPK LNNTTEKLPI LVYYHGGAFC LESAFSFLHQ
    110 120 130 140 150
    RYLNIVASKA NVLVVSIEYR LAPEHPLPAA YEDGWYALKW VTSHSTNNNK
    160 170 180 190 200
    PTNADPWLIK HGDFNRFYIG GDTSGANIAH NAALRVGAEA LPGGLRIAGV
    210 220 230 240 250
    LSAFPLFWGS KPVLSEPVEG HEKSSPMQVW NFVYPDAPGG IDNPLINPLA
    260 270 280 290 300
    PGAPNLATLG CPKMLVFVAG KDDLRDRGIW YYEAVKESGW KGDVELAQYE
    310 320
    GEEHCFQIYH PETENSKDLI GRIASFLV
    Length:328
    Mass (Da):36,061
    Last modified:February 1, 2005 - v1
    Checksum:i4694C7E46C2202D4
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB154414 mRNA. Translation: BAD80839.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB154414 mRNA. Translation: BAD80839.1.

    3D structure databases

    ProteinModelPortaliQ5NUF4.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    ESTHERiglyec-q5nuf4. Plant_carboxylesterase.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    UniPathwayiUPA00154.
    BRENDAi4.2.1.105. 2486.
    SABIO-RKQ5NUF4.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR013094. AB_hydrolase_3.
    [Graphical view]
    PfamiPF07859. Abhydrolase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHIDM_GLYEC
    AccessioniPrimary (citable) accession number: Q5NUF4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 16, 2013
    Last sequence update: February 1, 2005
    Last modified: February 17, 2016
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.