Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2-hydroxyisoflavanone dehydratase

Gene

HIDH

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dehydratase that mediates the biosynthesis of isoflavonoids. Can use both 4'-hydroxylated and 4'-methoxylated 2-hydroxyisoflavanones as substrates. Has also a slight carboxylesterase activity toward p-nitrophenyl butyrate.1 Publication

Catalytic activityi

2,7,4'-trihydroxyisoflavanone = daidzein + H2O.1 Publication
2,4',5,7-tetrahydroxyisoflavanone = genistein + H2O.1 Publication
A carboxylic ester + H2O = an alcohol + a carboxylate.1 Publication

Kineticsi

kcat is 1.6 sec(-1) with 2,7-dihydroxy-4'-methoxyisoflavanone, 5.3 sec(-1) with 2,7,4'-trihydroxyisoflavanone and 18.1 sec(-1) with 2,5,7,4'-tetrahydroxyisoflavanone as substrates, respectively (at pH 7.5 and 30 degrees Celsius).

  1. KM=29 µM for 2,7-dihydroxy-4'-methoxyisoflavanone (at pH 7.5 and 30 degrees Celsius)1 Publication
  2. KM=114 µM for 2,7,4'-trihydroxyisoflavanone (at pH 7.5 and 30 degrees Celsius)1 Publication
  3. KM=170 µM for 2,5,7,4'-tetrahydroxyisoflavanone (at pH 7.5 and 30 degrees Celsius)1 Publication

    Pathwayi: flavonoid biosynthesis

    This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei164 – 16411 Publication
    Active sitei263 – 26311 Publication
    Active sitei295 – 29511 Publication

    GO - Molecular functioni

    • 2-hydroxyisoflavanone dehydratase activity Source: UniProtKB
    • carboxylic ester hydrolase activity Source: UniProtKB

    GO - Biological processi

    • flavonoid biosynthetic process Source: UniProtKB-UniPathway
    • isoflavonoid biosynthetic process Source: UniProtKB
    • isoflavonoid metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Lyase

    Keywords - Biological processi

    Flavonoid biosynthesis

    Enzyme and pathway databases

    BRENDAi4.2.1.105. 2483.
    SABIO-RKQ5NUF3.
    UniPathwayiUPA00154.

    Protein family/group databases

    ESTHERisoybn-q5nuf3. Plant_carboxylesterase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-hydroxyisoflavanone dehydratase (EC:3.1.1.1, EC:4.2.1.105)
    Alternative name(s):
    Carboxylesterase HIDH
    Gene namesi
    Name:HIDH
    Ordered Locus Names:Glyma01g45020
    OrganismiGlycine max (Soybean) (Glycine hispida)
    Taxonomic identifieri3847 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
    Proteomesi
    • UP000008827 Componentsi: Chromosome 1, Unassembled WGS sequence

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi78 – 781G → A: Reduction of both dehydratase and carboxylesterase activities. 1 Publication
    Mutagenesisi79 – 791G → A: Reduction of both dehydratase and carboxylesterase activities. 1 Publication
    Mutagenesisi164 – 1641T → A or S: Reduction of both dehydratase and carboxylesterase activities. 1 Publication
    Mutagenesisi263 – 2631D → N: Complete loss of both dehydratase and carboxylesterase activities. 1 Publication
    Mutagenesisi295 – 2951H → A: Complete loss of both dehydratase and carboxylesterase activities. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3193192-hydroxyisoflavanone dehydratasePRO_0000424101Add
    BLAST

    Proteomic databases

    PRIDEiQ5NUF3.

    Expressioni

    Gene expression databases

    GenevisibleiQ5NUF3. GM.

    Interactioni

    Protein-protein interaction databases

    STRINGi3847.GLYMA01G45020.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5NUF3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi77 – 793Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole1 Publication

    Sequence similaritiesi

    Belongs to the 'GDXG' lipolytic enzyme family.Curated

    Phylogenomic databases

    eggNOGiKOG1515. Eukaryota.
    COG0657. LUCA.
    InParanoidiQ5NUF3.
    KOiK13258.
    OMAiAYEDGWT.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR013094. AB_hydrolase_3.
    IPR002168. Lipase_GDXG_HIS_AS.
    [Graphical view]
    PfamiPF07859. Abhydrolase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS01173. LIPASE_GDXG_HIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5NUF3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAKEIVKELL PLIRVYKDGS VERLLSSENV AASPEDPQTG VSSKDIVIAD
    60 70 80 90 100
    NPYVSARIFL PKSHHTNNKL PIFLYFHGGA FCVESAFSFF VHRYLNILAS
    110 120 130 140 150
    EANIIAISVD FRLLPHHPIP AAYEDGWTTL KWIASHANNT NTTNPEPWLL
    160 170 180 190 200
    NHADFTKVYV GGETSGANIA HNLLLRAGNE SLPGDLKILG GLLCCPFFWG
    210 220 230 240 250
    SKPIGSEAVE GHEQSLAMKV WNFACPDAPG GIDNPWINPC VPGAPSLATL
    260 270 280 290 300
    ACSKLLVTIT GKDEFRDRDI LYHHTVEQSG WQGELQLFDA GDEEHAFQLF
    310
    KPETHLAKAM IKRLASFLV
    Length:319
    Mass (Da):35,138
    Last modified:February 1, 2005 - v1
    Checksum:iE8333CF425FBA4A3
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB154415 mRNA. Translation: BAD80840.1.
    BT097440 mRNA. Translation: ACU22699.1.
    CM000834 Genomic DNA. No translation available.
    RefSeqiNP_001237228.1. NM_001250299.2.
    UniGeneiGma.19376.

    Genome annotation databases

    EnsemblPlantsiGLYMA01G45020.1; GLYMA01G45020.1; GLYMA01G45020.
    GeneIDi547489.
    GrameneiGLYMA01G45020.1; GLYMA01G45020.1; GLYMA01G45020.
    KEGGigmx:547489.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB154415 mRNA. Translation: BAD80840.1.
    BT097440 mRNA. Translation: ACU22699.1.
    CM000834 Genomic DNA. No translation available.
    RefSeqiNP_001237228.1. NM_001250299.2.
    UniGeneiGma.19376.

    3D structure databases

    ProteinModelPortaliQ5NUF3.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3847.GLYMA01G45020.1.

    Protein family/group databases

    ESTHERisoybn-q5nuf3. Plant_carboxylesterase.

    Proteomic databases

    PRIDEiQ5NUF3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiGLYMA01G45020.1; GLYMA01G45020.1; GLYMA01G45020.
    GeneIDi547489.
    GrameneiGLYMA01G45020.1; GLYMA01G45020.1; GLYMA01G45020.
    KEGGigmx:547489.

    Phylogenomic databases

    eggNOGiKOG1515. Eukaryota.
    COG0657. LUCA.
    InParanoidiQ5NUF3.
    KOiK13258.
    OMAiAYEDGWT.

    Enzyme and pathway databases

    UniPathwayiUPA00154.
    BRENDAi4.2.1.105. 2483.
    SABIO-RKQ5NUF3.

    Gene expression databases

    GenevisibleiQ5NUF3. GM.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR013094. AB_hydrolase_3.
    IPR002168. Lipase_GDXG_HIS_AS.
    [Graphical view]
    PfamiPF07859. Abhydrolase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS01173. LIPASE_GDXG_HIS. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis."
      Akashi T., Aoki T., Ayabe S.
      Plant Physiol. 137:882-891(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, MOTIF, MUTAGENESIS OF GLY-78; GLY-79; THR-164; ASP-263 AND HIS-295, BIOPHYSICOCHEMICAL PROPERTIES.
      Tissue: Seedling.
    2. Cheung F., Xiao Y., Chan A., Moskal W., Town C.D.
      Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Williams 82.

    Entry informationi

    Entry nameiHIDH_SOYBN
    AccessioniPrimary (citable) accession number: Q5NUF3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 16, 2013
    Last sequence update: February 1, 2005
    Last modified: March 16, 2016
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.