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Protein

Nuclear factor erythroid 2-related factor 2

Gene

NFE2L2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor erythroid 2-related factor 2
Short name:
NF-E2-related factor 2
Short name:
NFE2-related factor 2
Alternative name(s):
Nuclear factor, erythroid derived 2, like 2
Gene namesi
Name:NFE2L2
Synonyms:NRF2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Nucleus PROSITE-ProRule annotation

  • Note: Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 607607Nuclear factor erythroid 2-related factor 2PRO_0000286396Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei40 – 401Phosphoserine; by PKCBy similarity
Modified residuei214 – 2141PhosphoserineBy similarity
Modified residuei598 – 5981N6-acetyllysine; by CREBBPBy similarity
Modified residuei601 – 6011N6-acetyllysine; by CREBBPBy similarity

Post-translational modificationi

Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.By similarity
Acetylation at Lys-598 and Lys-601 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence.By similarity
Ubiquitinated by the KEAP1-CUL3-RBX1 E3 ubiquitin ligase complex and subject to proteasomal degradation. Ubiquitination is inhibited by sulforaphane (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5NUA6.
PRIDEiQ5NUA6.

Interactioni

Subunit structurei

Heterodimer. Forms a ternary complex with PGAM5 and KEAP1. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1. Interacts with EEF1D at heat shock promoter elements (HSE). Interacts (via the bZIP domain) with MAFK; required for binding to antioxidant response (ARE) elements on DNA. Interacts with CHD6; involved in activation of the transcription (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025637.

Structurei

3D structure databases

ProteinModelPortaliQ5NUA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini499 – 56264bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni501 – 52020Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni524 – 5318Leucine-zipperPROSITE-ProRule annotation
Regioni593 – 5986Mediates interaction with CHD6 and is necessary to activate transcriptionBy similarity

Domaini

Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.

Sequence similaritiesi

Belongs to the bZIP family. CNC subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410ISV3. Eukaryota.
ENOG41100FB. LUCA.
HOGENOMiHOG000234410.
HOVERGENiHBG052609.
InParanoidiQ5NUA6.

Family and domain databases

Gene3Di1.10.880.10. 1 hit.
InterProiIPR004827. bZIP.
IPR004826. bZIP_Maf.
IPR029845. Nrf2.
IPR008917. TF_DNA-bd.
[Graphical view]
PANTHERiPTHR24411:SF3. PTHR24411:SF3. 1 hit.
PfamiPF03131. bZIP_Maf. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
SUPFAMiSSF47454. SSF47454. 1 hit.
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5NUA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMDLELPPPG LPSQQDMDLI DILWRQDIDL GVSREVFDFS QRQKEHELEK
60 70 80 90 100
QKKLEKERQE QLQKEQEKAF FAQLQLDEET GEFLPIQPAQ HIPSETSGSA
110 120 130 140 150
NYSQVAPIPK ADDLYFDDCM QLLAETFPFV DDNEVSSATF QSLVPDIPSH
160 170 180 190 200
IESPVFTAPP QAQSPETLIV QVATAVLDDM QDIEQVWEEL LSIPELQCLN
210 220 230 240 250
IQNDKLAETS TVPSPETKLT EIDNYHFYSS MPSLDKEVGN CSPHFLNAFE
260 270 280 290 300
DSFNSILSTE DSSQLTVNSL NSSATVNTDF GDEFYSAFIA EPSTSNGMPS
310 320 330 340 350
SATLSQSLSE LLNGPIDLSD LSLCKAFNQN HPESTTAEFN DSDSGISLNT
360 370 380 390 400
TSPSMASPDH SVESSIYGDT LLGFSDSEME EIDSTPGNVK QKGPKTPSVW
410 420 430 440 450
PPGDPVQPLS SSQGNSAAAR DSQCENAPKK EVPVSPGHRK TPFTKDKHSS
460 470 480 490 500
RLEAHLTRDE LRAKALHIPF PVEKIINLPV EDFNEMMSKE QFNEAQLALI
510 520 530 540 550
RDIRRRGKNK VAAQNCRKRK LENIVELEQD LDHLKDEKEK LLKERGENDK
560 570 580 590 600
SLHLLKKQLS TLYLEVFSML RDENGKPYSP SEYSLQQTSD GNVFLVPKSK

KPDTKKN
Length:607
Mass (Da):67,895
Last modified:May 1, 2007 - v2
Checksum:iE0C576DBAF2A1A21
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti287 – 2871A → T in BAD81030 (Ref. 1) Curated
Sequence conflicti423 – 4231Q → H in BAD81030 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB162435 mRNA. Translation: BAD81030.1.
BC102275 mRNA. Translation: AAI02276.1. Different termination.
UniGeneiBt.17324.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB162435 mRNA. Translation: BAD81030.1.
BC102275 mRNA. Translation: AAI02276.1. Different termination.
UniGeneiBt.17324.

3D structure databases

ProteinModelPortaliQ5NUA6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025637.

Proteomic databases

PaxDbiQ5NUA6.
PRIDEiQ5NUA6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410ISV3. Eukaryota.
ENOG41100FB. LUCA.
HOGENOMiHOG000234410.
HOVERGENiHBG052609.
InParanoidiQ5NUA6.

Family and domain databases

Gene3Di1.10.880.10. 1 hit.
InterProiIPR004827. bZIP.
IPR004826. bZIP_Maf.
IPR029845. Nrf2.
IPR008917. TF_DNA-bd.
[Graphical view]
PANTHERiPTHR24411:SF3. PTHR24411:SF3. 1 hit.
PfamiPF03131. bZIP_Maf. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
SUPFAMiSSF47454. SSF47454. 1 hit.
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNF2L2_BOVIN
AccessioniPrimary (citable) accession number: Q5NUA6
Secondary accession number(s): Q3T0S8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: March 16, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.