Reviewed, UniProtKB/Swiss-Prot Q5NU32 (AOFA_HORSE)

Names and origin

Protein names

Recommended name:
    Amine oxidase [flavin-containing] A
    EC=1.4.3.4
Alternative name(s):
    Monoamine oxidase type A
      Short name=MAO-A

Gene names

Name:

MAOA

Organism

Equus caballus (Horse)

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine By similarity.
Catalytic activity	RCH(2)NHR' + H(2)O + O(2) = RCHO + R'NH(2) + H(2)O(2).
Cofactor	FAD By similarity.
Subunit structure	Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic sideBy similarity.
Sequence similarities	Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Biological process	Catecholamine metabolism Neurotransmitter degradation
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	catecholamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amine oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	Amine oxidase [flavin-containing] A		PRO_0000099849
Regions
	Topological domain	1 – 497	497	Cytoplasmic Potential
	Transmembrane	498 – 518	21	Anchor for type IV membrane protein Potential
	Topological domain	519 – 527	9	Mitochondrial intermembrane Potential
Sites
	Site	335	1	Important for substrate specificity By similarity
	Site	374	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	406	1	S-8alpha-FAD cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NU32-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 177988F29564E35B	FASTA	527	59,580
	10 20 30 40 50 60 MASQEKASMA GHMFDVVVIG GGISGLSAAK LLAEHETNVL VLEARDRVGG RTYTVRNKHV 70 80 90 100 110 120 NYVDVGGAYV GPTQNRILRL SKELGLETYK VNVNERLVQY VKGKSYPFRG AFPPVWNPIA 130 140 150 160 170 180 YLDYNNLWRT MDNMGKEIPA DAPWEAPHAE EWDKMTMKDL IDKICWTKTA RQFASLFVNI 190 200 210 220 230 240 NVTSEPHQVS ALWFLWYVKQ CGGTTRIFSI TNGGQERKFV GGSGQVSERI MELLGDRVKL 250 260 270 280 290 300 EHPVTYVDQS GDNIIVETLN HEHFECKYVI SAIPPALTAK IHFKPELPSE RNQLIQRLPM 310 320 330 340 350 360 GAIIKCMMYY KEAFWKKKDY CGSMIIEDEE APISITLDDS KPDGSLPAIM GFILARKADR 370 380 390 400 410 420 LAKLHKEMRK KKICELYAKV LGSQEALQPV HYEEKNWCEE QYSGGCYTAY FPPGIMTQYG 430 440 450 460 470 480 RVIRQPVGRI YFAGTETATR WSGYMEGAVE AGERAAREIL NALGKVAEKD IWLQEPESKD 490 500 510 520 VPAVEITHSF WERNLPSVGG LLKIIGFSTS ITALWIVVYK FKLLTRS

References

[1]	"Equus caballus monoamine oxidase A (MAOA), mRNA." Momozawa Y., Mori Y. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB178282 mRNA. Translation: BAD80721.1.
RefSeq	NP_001075301.1.
UniGene	Eca.12721
3D structure databases
SMR	Q5NU32. Positions 23-509.
ModBase	Search...
Genome annotation databases
GeneID	100033867.
Phylogenomic databases
HOVERGEN	Q5NU32.
Family and domain databases
InterPro	IPR001613. Amineoxid_fl. IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00757. AMINEOXDASEF.
ProtoNet	Search...

Entry information

Entry name

AOFA_HORSE

Accession

Primary (citable) accession number: Q5NU32

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	February 1, 2005
Last modified:	November 25, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)