Reviewed, UniProtKB/Swiss-Prot Q5NRM2 (CYSI_ZYMMO)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	ZMO0008

Organism

Zymomonas mobilis [Complete proteome] [HAMAP]

Taxonomic identifier

542 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH.
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	Sulfite reductase [NADPH] hemoprotein beta-component		PRO_0000199919
Sites
	Metal binding	434	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	440	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	479	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	483	1	Iron (siroheme axial ligand) By similarity
	Metal binding	483	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NRM2-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: BA050FC80FC53326	FASTA	570	64,064
	10 20 30 40 50 60 MTQKHPQTLV VDAPLSDAER LKRESNFLRG TIAEDLNDGL TGGFNGDNST LIRFHGMYQQ 70 80 90 100 110 120 DDRDIRAERA EEKLEPRYAM MLRCRLPGGI ISPEQWLKMD SFASEKTLYG SIRLTNRQTF 130 140 150 160 170 180 QFHGILKKDL KTVHHLLHES GLDSLATAND VNRNVLCTSN PVESDLHAEA YEWAKKISEH 190 200 210 220 230 240 LLPQTHAYAE IWLDQEKLAT TDQEPILGAT YLPRKFKTTV VIPPQNDVDI YANDLNFIAI 250 260 270 280 290 300 SENDQLVGFN VLIGGGLSIT IGDKTTHPGV AKDIGYIEKE KVLAVAEAVV TTQRDWGNRS 310 320 330 340 350 360 NRKNARLRYT LERVGLDVFK AEVEKRAGVT FEASRPFTLT GRGDRFGWVK GIDNQWHLTL 370 380 390 400 410 420 FVENGRLLDY PHRPLKSGIA EIAKVHKGDF RLTANQNLIV AGVSEEDKDT IEKIARDHGL 430 440 450 460 470 480 LENISELRKN SMACVSFPTC PLAMAEAERF LPNFLTKVEA VMTKYDIADE YIVFRTTGCP 490 500 510 520 530 540 NNCGRSLLAE IGLVGRAIGR YNLYIGGDRV GTRIPRLFRE NITEDEILSE VDSLIGRWAK 550 560 570 ERDGKEAFGD FVVRAGIVKA VTDPAIDFYD

References

[1]

"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed: 15592456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008692 Genomic DNA. Translation: AAV88632.1.
RefSeq	YP_161743.1.
3D structure databases
SMR	Q5NRM2. Positions 81-570.
ModBase	Search...
Genome annotation databases
GeneID	3189461.
GenomeReviews	Gene locus ZMO0008 in contig AE008692_GR.
KEGG	zmo:ZMO0008.
NMPDR	fig|264203.3.peg.504.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5NRM2.
Enzyme and pathway databases
BioCyc	ZMOB264203:ZMO0008-MON.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_ZYMMO

Accession

Primary (citable) accession number: Q5NRM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	February 1, 2005
Last modified:	November 25, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)