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Q5NRM2 (CYSI_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Sulfite reductase [NADPH] hemoprotein beta-component

Short name=SiR-HP
Short name=SiRHP
EC=1.8.1.2
Gene names
Name:cysI
Ordered Locus Names:ZMO0008
OrganismZymomonas mobilis [Complete proteome] [HAMAP]
Taxonomic identifier542 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas

Protein attributes

Sequence length570 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity. HAMAP MF_01540

Catalytic activity

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540

Cofactor

Binds 1 siroheme per subunit By similarity. HAMAP MF_01540

Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP MF_01540

Pathway

Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP MF_01540

Subunit structure

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.

Sequence similarities

Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 570570Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540
PRO_0000199919

Sites

Metal binding4341Iron-sulfur (4Fe-4S) By similarity
Metal binding4401Iron-sulfur (4Fe-4S) By similarity
Metal binding4791Iron-sulfur (4Fe-4S) By similarity
Metal binding4831Iron (siroheme axial ligand) By similarity
Metal binding4831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5NRM2 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: BA050FC80FC53326

FASTA57064,064
        10         20         30         40         50         60 
MTQKHPQTLV VDAPLSDAER LKRESNFLRG TIAEDLNDGL TGGFNGDNST LIRFHGMYQQ 

        70         80         90        100        110        120 
DDRDIRAERA EEKLEPRYAM MLRCRLPGGI ISPEQWLKMD SFASEKTLYG SIRLTNRQTF 

       130        140        150        160        170        180 
QFHGILKKDL KTVHHLLHES GLDSLATAND VNRNVLCTSN PVESDLHAEA YEWAKKISEH 

       190        200        210        220        230        240 
LLPQTHAYAE IWLDQEKLAT TDQEPILGAT YLPRKFKTTV VIPPQNDVDI YANDLNFIAI 

       250        260        270        280        290        300 
SENDQLVGFN VLIGGGLSIT IGDKTTHPGV AKDIGYIEKE KVLAVAEAVV TTQRDWGNRS 

       310        320        330        340        350        360 
NRKNARLRYT LERVGLDVFK AEVEKRAGVT FEASRPFTLT GRGDRFGWVK GIDNQWHLTL 

       370        380        390        400        410        420 
FVENGRLLDY PHRPLKSGIA EIAKVHKGDF RLTANQNLIV AGVSEEDKDT IEKIARDHGL 

       430        440        450        460        470        480 
LENISELRKN SMACVSFPTC PLAMAEAERF LPNFLTKVEA VMTKYDIADE YIVFRTTGCP 

       490        500        510        520        530        540 
NNCGRSLLAE IGLVGRAIGR YNLYIGGDRV GTRIPRLFRE NITEDEILSE VDSLIGRWAK 

       550        560        570 
ERDGKEAFGD FVVRAGIVKA VTDPAIDFYD 

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References

[1]"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4."
Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. expand/collapse author list , Kang H.L., Lee S.Y., Lee K.J., Kang H.S.
Nat. Biotechnol. 23:63-68(2005) [PubMed: 15592456] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008692 Genomic DNA. Translation: AAV88632.1.
RefSeqYP_161743.1. NC_006526.2.

3D structure databases

ProteinModelPortalQ5NRM2.
SMRQ5NRM2. Positions 81-570.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3189461.
GenomeReviewsGene locus ZMO0008 in contig AE008692_GR.
KEGGzmo:ZMO0008.
NMPDRfig|264203.3.peg.504.
PATRIC32565380. VBIZymMob102260_0008.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG626671.
OMAWIEGEDG.
ProtClustDBPRK13504.

Enzyme and pathway databases

BioCycZMOB264203:ZMO0008-MONOMER.

Family and domain databases

HAMAPMF_01540. CysI.
[Tree]
InterProIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
Gene3DG3DSA:3.90.480.10. G3DSA:3.90.480.10. 2 hits.
KOK00381.
PfamPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSPR00397. SIROHAEM.
SUPFAMSSF55124. NiR_SiRalpha_1/3. 2 hits.
TIGRFAMsTIGR02041. CysI. 1 hit.
PROSITEPS00365. NIR_SIR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSI_ZYMMO
AccessionPrimary (citable) accession number: Q5NRM2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: February 1, 2005
Last modified: January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families