Q5NRD6 (BIOB_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	ZMO0094

Organism

Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) [Complete proteome] [HAMAP]

Taxonomic identifier

264203 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas › Zymomonas mobilis › Zymomonas mobilis subsp. mobilis

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Biotin synthase HAMAP-Rule MF_01694		PRO_0000381729
Sites
	Metal binding	74	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	78	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	81	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	118	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	150	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	210	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	282	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NRD6 [UniParc]. Last modified March 23, 2010. Version 2. Checksum: B02CEABF178FF96B	FASTA	335	36,263
	10 20 30 40 50 60 MVSVGTQSHS GRDQAEQNPS VENVRYDWQL DEALAIYNLP LLELIGRANS VHRRYHDAGH 70 80 90 100 110 120 LQKSSLLSIK TGGCPEDCGY CSQSAHHDVE LTREKLMNPT AVIGLAAKAK KAGAERFCMG 130 140 150 160 170 180 AAWRKVRDGK EFDAVLEMIR GVRALDMEAC VTLGMVNEDQ ARRLAEAGLT AYNHNLDTGP 190 200 210 220 230 240 AYYPQIVSTH SYQDRLETLA KIRDAGIALC TGGIIGLGES PRDRVEMLVV LAGMNPHPES 250 260 270 280 290 300 VPVNMLVPIE GTPLADADPV DPLEIVRMIA TARLMMPQSM VRLSAGRSTL SRETQILCLV 310 320 330 AGANSIFYGN VLLTTPNADM AADDALLEAL GVTAE

References

[1]

"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008692 Genomic DNA. Translation: AAV88718.2.
RefSeq	YP_161829.2. NC_006526.2.
3D structure databases
HSSP	HSSP built from PDB template 1R30 based on UniProtKB P12996.
ProteinModelPortal	Q5NRD6.
ModBase	Search...
Protein-protein interaction databases
STRING	264203.ZMO0094.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV88718; AAV88718; ZMO0094.
GeneID	3188413.
KEGG	zmo:ZMO0094.
PATRIC	32565540. VBIZymMob102260_0088.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HOG000239957.
KO	K01012.
OMA	MKETKQD.
ProtClustDB	CLSK2524463.
Enzyme and pathway databases
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_ZYMMO

Accession

Primary (citable) accession number: Q5NRD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 23, 2010
Last modified:	May 1, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program