Q5NR44 (SYC_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	ZMO0186

Organism

Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) [Complete proteome] [HAMAP]

Taxonomic identifier

264203 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas › Zymomonas mobilis › Zymomonas mobilis subsp. mobilis

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 516	516	Cysteine--tRNA ligase HAMAP-Rule MF_00041		PRO_0000159531
Regions
	Motif	36 – 46	11	"HIGH" region HAMAP-Rule MF_00041
	Motif	285 – 289	5	"KMSKS" region HAMAP-Rule MF_00041
Sites
	Metal binding	34	1	Zinc By similarity
	Metal binding	225	1	Zinc By similarity
	Metal binding	250	1	Zinc By similarity
	Metal binding	254	1	Zinc By similarity
	Binding site	288	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NR44 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 692EF2DF045E8CA6	FASTA	516	58,524
	10 20 30 40 50 60 MAKPSAPVLF FNSLSKKIEN FTPIDPERVR LYSCGPTVYN FAHLGNLRAY VFTDSLRRML 70 80 90 100 110 120 NWKGYPVNHV INITDVGHLT SDSDTGDDKM EAAAHKAQKT IWDVARFYTE AFWSDLKNLN 130 140 150 160 170 180 IFNPTIWSIA TDHIEDMIAF ARKIEEAGFT YQLDSGLYFD TSRLKEYGLL AGHREAEGVG 190 200 210 220 230 240 RIETVAGKKH PADFALWRRS SPDEKRQMEW ISPWGPGAPG WHLECSVMSM KYLGEQFDIH 250 260 270 280 290 300 TGGIDHREIH HCNEIAQNQA FTGDQQKTGA NFWLHNNFLV DRSGKMSKSK GSFLTLSTLI 310 320 330 340 350 360 EAGIHPLAYR VLCLSAHYRS ELEFSPDNIL AALTRLKRLV IAVGQLKKKV QKKVVADGEP 370 380 390 400 410 420 DWLRAFDERS FSKGAPLDYQ RGLIEAPLKK EALAILERFD QAVSNDLMLP QALPLLEETL 430 440 450 460 470 480 GNKKLQPEEQ LRLIASMDMI LGLNLLHLDR ASLNIRPKNA SLQENNVLAL LEERQLKRKE 490 500 510 KDFAASDAIR NRLSEAGIDV LDGDPLGWEW RPEWNA

References

[1]

"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008692 Genomic DNA. Translation: AAV88810.1.
RefSeq	YP_161921.1. NC_006526.2.
3D structure databases
ProteinModelPortal	Q5NR44.
ModBase	Search...
Protein-protein interaction databases
STRING	264203.ZMO0186.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV88810; AAV88810; ZMO0186.
GeneID	3187427.
KEGG	zmo:ZMO0186.
PATRIC	32565724. VBIZymMob102260_0179.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HOG000245250.
KO	K01883.
OMA	GGEDHIA.
ProtClustDB	CLSK835090.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00041. Cys_tRNA_synth.
InterPro	IPR015803. Cys-tRNA-ligase. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR10890. PTHR10890. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ZYMMO

Accession

Primary (citable) accession number: Q5NR44

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program