Q5NR24 (PROB_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	ZMO0206

Organism

Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) [Complete proteome] [HAMAP]

Taxonomic identifier

264203 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas › Zymomonas mobilis › Zymomonas mobilis subsp. mobilis

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Glutamate 5-kinase HAMAP-Rule MF_00456		PRO_0000109764
Regions
	Domain	306 – 383	78	PUA
	Nucleotide binding	200 – 201	2	ATP By similarity
	Nucleotide binding	243 – 249	7	ATP By similarity
Sites
	Binding site	40	1	ATP By similarity
	Binding site	80	1	Substrate By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	180	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NR24 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 17DE6AC6F8BFA994	FASTA	397	42,509
	10 20 30 40 50 60 MVADLTSDIS ESQEQETETN SANNNGAVFR PENCPRLVIK IGSSLLVDQR GQVRRDWLQT 70 80 90 100 110 120 VAYDIAKLHQ AGQQIIVVSS GAIALGARRL NLPRGGRASL EDAQASASVG QILLSQCWAE 130 140 150 160 170 180 LLGACSLDSS QILLTLDDLE DRRRYLNVSA TLDRLLSLGV VPVINENDSI ATAEIRFGDN 190 200 210 220 230 240 DRLAARIGQA SHASGVILFS DVDGLYTANP MKDPNAKRID RVEHIDNSTE AMASSDSASG 250 260 270 280 290 300 MGSGGMASKV EAARIATYSG VNLAITTGKR PSPLTMFLDD GAGTLFTADE SASAHKTWLA 310 320 330 340 350 360 GRLTAHGQVY IDQGAVEALH DGNSLLPAGV CSIEGKFNRG DVVDILDQEH NLIARGLIEY 370 380 390 DSEDSAQITG KRSQEIADIL GYEARTALIH RNHMVML

References

[1]

"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008692 Genomic DNA. Translation: AAV88830.1.
RefSeq	YP_161941.1. NC_006526.2.
3D structure databases
ProteinModelPortal	Q5NR24.
ModBase	Search...
Protein-protein interaction databases
STRING	264203.ZMO0206.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV88830; AAV88830; ZMO0206.
GeneID	3187511.
KEGG	zmo:ZMO0206.
PATRIC	32565768. VBIZymMob102260_0200.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HOG000246368.
KO	K00931.
OMA	DHLQLRG.
ProtClustDB	PRK05429.
Enzyme and pathway databases
UniPathway	UPA00098; UER00359.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit.
HAMAP	MF_00456. ProB.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_ZYMMO

Accession

Primary (citable) accession number: Q5NR24

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program