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Q5NQQ7

- SYI_ZYMMO

UniProt

Q5NQQ7 - SYI_ZYMMO

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Protein

Isoleucine--tRNA ligase

Gene
ileS, ZMO0323
Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei589 – 5891Aminoacyl-adenylate By similarity
Binding sitei633 – 6331ATP By similarity
Metal bindingi915 – 9151Zinc By similarity
Metal bindingi918 – 9181Zinc By similarity
Metal bindingi932 – 9321Zinc By similarity
Metal bindingi935 – 9351Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:ZMO0323
OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Taxonomic identifieri264203 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
ProteomesiUP000001173: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 941941Isoleucine--tRNA ligaseUniRule annotationPRO_0000098513Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi264203.ZMO0323.

Structurei

3D structure databases

ProteinModelPortaliQ5NQQ7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 7911"HIGH" regionUniRule annotationAdd
BLAST
Motifi630 – 6345"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5NQQ7-1 [UniParc]FASTAAdd to Basket

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MSDQKSADAT QARDWRPTVF LPKTSFPMKA GLAKKEPEIL ARWQKEDLYQ    50
QLREQRKGAE RFILHDGPPY ANGDIHIGHA LNKILKDIIM RSQTLLGKDV 100
PYIPGWDCHG LPIEWKVEEQ FRKKKLNVDK DVNAVEFRQE CRKYAVHWVD 150
TQRQEFKRLG VLGEWDNPYL TMNFEAEAII AGELMRFSET GQIYRGAKPV 200
LWSVVEKTAL AEAEVDYADV DSTTIDLAFK ITDSKIPELV GGYAVIWTTT 250
PWTIPANRAL AYGPDIDYVL VDLNGKHYLF AEALLEDSLK RIGHEGDAPV 300
LWRGKGAELD GSIAQHPMFE KGGFFAEPRP FLGGSHVTTE AGTGIVHMAP 350
DYGEDDFLLC KAHNIDPVFA VEDDGRYRKD WLWMGGEGLV ISPKINAADG 400
PICSDLREVG ALLATSVLHH SYPHSWRSKA KLIYRCTPQW FIALDRPVEK 450
GAIAGKTLRE TALKAIDEVS WFPAKGKNRI QTMVEGRPDW VISRQRAWGV 500
PITLYVNRES GDYLRDPEVN ARILAAFRKE GADAWFKANH QLFLGDKYRL 550
EDYEPVNDIL DVWFDSGSTH AFVVEARYGE GTRAQLYLEG SDQHRGWFQS 600
SLLESCGSRG HAPYEAVLTH GFTLDGTGRK MSKSVGNVID PLKVINESGA 650
DILRMWVAST DYNEDVRISK EVLSGTSDGY RKLRNSFRYL LGALEGFSEE 700
EKVDLADLPE LEKYILHLLA ELDQALHESV NGFAFNRYLR LLSDFVNNDL 750
SAFFFDIRKD RLYCDVGEAA PQGTEERRAY RTVLDILFHA LVRYAAPILC 800
FTAEEVWLHR FPDAGSVHLS VWPEVDGQWK NAVLGEKWAV IREQRQIVTE 850
KIEPLRREKI VGSSLEAEVT LPVDAATAKI LSSVDFSEIC ITAKINLVDA 900
SDAAITVDRT QNHKCGRCWQ HLPEVEEDGA LCDRCKSVVG E 941
Length:941
Mass (Da):106,263
Last modified:February 1, 2005 - v1
Checksum:i645DF7E9C0E82279
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 943SQT → TKP in AAD56932. 1 Publication
Sequence conflicti140 – 1401E → K in AAD56932. 1 Publication
Sequence conflicti181 – 1811A → S in AAD56932. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF180145 Genomic DNA. Translation: AAD56932.1.
AE008692 Genomic DNA. Translation: AAV88947.1.
RefSeqiYP_162058.1. NC_006526.2.

Genome annotation databases

EnsemblBacteriaiAAV88947; AAV88947; ZMO0323.
GeneIDi3188160.
KEGGizmo:ZMO0323.
PATRICi32565986. VBIZymMob102260_0307.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF180145 Genomic DNA. Translation: AAD56932.1 .
AE008692 Genomic DNA. Translation: AAV88947.1 .
RefSeqi YP_162058.1. NC_006526.2.

3D structure databases

ProteinModelPortali Q5NQQ7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264203.ZMO0323.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV88947 ; AAV88947 ; ZMO0323 .
GeneIDi 3188160.
KEGGi zmo:ZMO0323.
PATRICi 32565986. VBIZymMob102260_0307.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Lee H.J., Kang H.S.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 31821 / ZM4 / CP4.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31821 / ZM4 / CP4.

Entry informationi

Entry nameiSYI_ZYMMO
AccessioniPrimary (citable) accession number: Q5NQQ7
Secondary accession number(s): Q9RNJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 1, 2005
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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