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Q5NQQ7

- SYI_ZYMMO

UniProt

Q5NQQ7 - SYI_ZYMMO

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei589 – 5891Aminoacyl-adenylateUniRule annotation
    Binding sitei633 – 6331ATPUniRule annotation
    Metal bindingi915 – 9151ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation
    Metal bindingi932 – 9321ZincUniRule annotation
    Metal bindingi935 – 9351ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:ZMO0323
    OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
    Taxonomic identifieri264203 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
    ProteomesiUP000001173: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 941941Isoleucine--tRNA ligasePRO_0000098513Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi264203.ZMO0323.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5NQQ7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi69 – 7911"HIGH" regionAdd
    BLAST
    Motifi630 – 6345"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5NQQ7-1 [UniParc]FASTAAdd to Basket

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    MSDQKSADAT QARDWRPTVF LPKTSFPMKA GLAKKEPEIL ARWQKEDLYQ    50
    QLREQRKGAE RFILHDGPPY ANGDIHIGHA LNKILKDIIM RSQTLLGKDV 100
    PYIPGWDCHG LPIEWKVEEQ FRKKKLNVDK DVNAVEFRQE CRKYAVHWVD 150
    TQRQEFKRLG VLGEWDNPYL TMNFEAEAII AGELMRFSET GQIYRGAKPV 200
    LWSVVEKTAL AEAEVDYADV DSTTIDLAFK ITDSKIPELV GGYAVIWTTT 250
    PWTIPANRAL AYGPDIDYVL VDLNGKHYLF AEALLEDSLK RIGHEGDAPV 300
    LWRGKGAELD GSIAQHPMFE KGGFFAEPRP FLGGSHVTTE AGTGIVHMAP 350
    DYGEDDFLLC KAHNIDPVFA VEDDGRYRKD WLWMGGEGLV ISPKINAADG 400
    PICSDLREVG ALLATSVLHH SYPHSWRSKA KLIYRCTPQW FIALDRPVEK 450
    GAIAGKTLRE TALKAIDEVS WFPAKGKNRI QTMVEGRPDW VISRQRAWGV 500
    PITLYVNRES GDYLRDPEVN ARILAAFRKE GADAWFKANH QLFLGDKYRL 550
    EDYEPVNDIL DVWFDSGSTH AFVVEARYGE GTRAQLYLEG SDQHRGWFQS 600
    SLLESCGSRG HAPYEAVLTH GFTLDGTGRK MSKSVGNVID PLKVINESGA 650
    DILRMWVAST DYNEDVRISK EVLSGTSDGY RKLRNSFRYL LGALEGFSEE 700
    EKVDLADLPE LEKYILHLLA ELDQALHESV NGFAFNRYLR LLSDFVNNDL 750
    SAFFFDIRKD RLYCDVGEAA PQGTEERRAY RTVLDILFHA LVRYAAPILC 800
    FTAEEVWLHR FPDAGSVHLS VWPEVDGQWK NAVLGEKWAV IREQRQIVTE 850
    KIEPLRREKI VGSSLEAEVT LPVDAATAKI LSSVDFSEIC ITAKINLVDA 900
    SDAAITVDRT QNHKCGRCWQ HLPEVEEDGA LCDRCKSVVG E 941
    Length:941
    Mass (Da):106,263
    Last modified:February 1, 2005 - v1
    Checksum:i645DF7E9C0E82279
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti92 – 943SQT → TKP in AAD56932. 1 PublicationCurated
    Sequence conflicti140 – 1401E → K in AAD56932. 1 PublicationCurated
    Sequence conflicti181 – 1811A → S in AAD56932. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF180145 Genomic DNA. Translation: AAD56932.1.
    AE008692 Genomic DNA. Translation: AAV88947.1.
    RefSeqiYP_162058.1. NC_006526.2.

    Genome annotation databases

    EnsemblBacteriaiAAV88947; AAV88947; ZMO0323.
    GeneIDi3188160.
    KEGGizmo:ZMO0323.
    PATRICi32565986. VBIZymMob102260_0307.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF180145 Genomic DNA. Translation: AAD56932.1 .
    AE008692 Genomic DNA. Translation: AAV88947.1 .
    RefSeqi YP_162058.1. NC_006526.2.

    3D structure databases

    ProteinModelPortali Q5NQQ7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 264203.ZMO0323.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAV88947 ; AAV88947 ; ZMO0323 .
    GeneIDi 3188160.
    KEGGi zmo:ZMO0323.
    PATRICi 32565986. VBIZymMob102260_0307.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Lee H.J., Kang H.S.
      Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 31821 / ZM4 / CP4.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 31821 / ZM4 / CP4.

    Entry informationi

    Entry nameiSYI_ZYMMO
    AccessioniPrimary (citable) accession number: Q5NQQ7
    Secondary accession number(s): Q9RNJ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3