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Q5NQ32

- PNP_ZYMMO

UniProt

Q5NQ32 - PNP_ZYMMO

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi484 – 4841MagnesiumUniRule annotation
Metal bindingi490 – 4901MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:ZMO0549
OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Taxonomic identifieri264203 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
ProteomesiUP000001173: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 748748Polyribonucleotide nucleotidyltransferasePRO_0000329953Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264203.ZMO0549.

Structurei

3D structure databases

ProteinModelPortaliQ5NQ32.
SMRiQ5NQ32. Positions 616-688.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini551 – 61060KHUniRule annotationAdd
BLAST
Domaini620 – 68869S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5NQ32-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFDIKRQEID WGGKKLTLET GQVARQADGA VIATLGETVV LCAVTAAKTV
60 70 80 90 100
KEGQDFFPLT VHYQEKYSAA GRIPGGFFKR ERGATERETL ISRLIDRPIR
110 120 130 140 150
PLFPEGFYNE TLVIAQVMSY DGENEPDILA MIAASAALAL SGVPFLGPIG
160 170 180 190 200
AARVGYQDGE FILNPTLEQL EKSDLDLVVG ATRDAVMMVE SEANELPEEV
210 220 230 240 250
MLNAVSFAHE SLQPVIKAII NLAEQAAKEP WELVSYDDSA LAAKVEELCY
260 270 280 290 300
DNFDKAYRLT RKAERVDALS KAKAVLDEAF PEADPTEKLR IQKLAKKLEA
310 320 330 340 350
KIVRTAILKE GRRIDGRDLK TVRPIRSQVG FLPRTHGSAL FTRGETQALV
360 370 380 390 400
STTLGTADAE QMIDGLTGLH YERFMLHYNF PPYSVGEVGR FGAPGRREIG
410 420 430 440 450
HGKLAWRALH PVLPSKADFP YTIRVLSDIT ESNGSSSMAT VCGGCLALMD
460 470 480 490 500
AGVPLTRPVS GIAMGLILEK DGFAILSDIM GDEDHLGDMD FKVAGTEKGI
510 520 530 540 550
TSLQMDIKVA GITEEIMQKA LEQAKGGRAH ILGEMSKALG EVRSEISNLA
560 570 580 590 600
PRIETMSVPK DKIRDVIGTG GKVIREIVAT TGAKVDIEDD GTVRLSSSDP
610 620 630 640 650
ANIEAAREWI NGIVEEPEVG KIYNGKVVNI VDFGAFVNFM GGRDGLVHVS
660 670 680 690 700
EIKNERVNKV SDVLSEGQEV KVKVLEIDNR GKVRLSMRVV DQETGAELDD
710 720 730 740
NRPPRENAER RGGERPRRDR GPRRESGDRP ARRDMEPEFA PAFLRKDS
Length:748
Mass (Da):81,949
Last modified:February 1, 2005 - v1
Checksum:iEB8C43821F85F6DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008692 Genomic DNA. Translation: AAV89173.1.
RefSeqiYP_162284.1. NC_006526.2.

Genome annotation databases

EnsemblBacteriaiAAV89173; AAV89173; ZMO0549.
GeneIDi3188183.
KEGGizmo:ZMO0549.
PATRICi32566432. VBIZymMob102260_0525.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008692 Genomic DNA. Translation: AAV89173.1 .
RefSeqi YP_162284.1. NC_006526.2.

3D structure databases

ProteinModelPortali Q5NQ32.
SMRi Q5NQ32. Positions 616-688.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264203.ZMO0549.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV89173 ; AAV89173 ; ZMO0549 .
GeneIDi 3188183.
KEGGi zmo:ZMO0549.
PATRICi 32566432. VBIZymMob102260_0525.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31821 / ZM4 / CP4.

Entry informationi

Entry nameiPNP_ZYMMO
AccessioniPrimary (citable) accession number: Q5NQ32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 1, 2005
Last modified: November 26, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3