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Q5NPH0

- GLND_ZYMMO

UniProt

Q5NPH0 - GLND_ZYMMO

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:ZMO0766
    OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
    Taxonomic identifieri264203 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
    ProteomesiUP000001173: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 926926Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000231700Add
    BLAST

    Proteomic databases

    PRIDEiQ5NPH0.

    Interactioni

    Protein-protein interaction databases

    STRINGi264203.ZMO0766.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5NPH0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini523 – 61795HDUniRule annotationAdd
    BLAST
    Domaini737 – 81478ACT 1UniRule annotationAdd
    BLAST
    Domaini849 – 92678ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 379379UridylyltransferaseAdd
    BLAST
    Regioni380 – 736357Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261779.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5NPH0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPVSFLSLAS QPQIIDYPAI KEEIDSIKKV AAGDPVSEYR AVSLVLKQTV    50
    LKGREVIAEA LTRRPHQGRI AALSYAYLTD QIIQLALYAM VGDDEKATDN 100
    LVILAVGGYG RGEMALHSDV DIAFLSRRSP RKAQKKLIES LISLLWDVGL 150
    RVSQSHRSLS DMVKMAKKDI TIRTALLESR YLVGDKALFE EASTRFIQKV 200
    VAGSGRDFVA AKLLEWDERH LARGDSRYLV EPHLKEGKGG LRDLQSLFWI 250
    AKYLYLEKEA RHTVLTSPIL TDYALVKADL IAGHEAKRFR RCENFLWAVR 300
    CHLHILVKRP EERLNFDVQR SLAEKMGYRS KSGKSAVERF MHHYFLVTKM 350
    VGNLAALFID ALKVNHYLKP KSRRSGASKQ IDGFPVIQGE IVLDDDFFFR 400
    HNPAALITLF AVAYRHRLDI HPLTLRQAGR DARLIDEALQ NNPVCNAAFL 450
    TLFTLPYNPA PILKIMNNTG VLGRFIPDFG RIVAQMQFDM YHHYTVDEHA 500
    IRALDILWQI ENNKLEDLYP LGSRLFKQLN ARLVLYMALF LHDIAKGREG 550
    SHSALGAEIA LRLCPRFGLT AAETKLVAWL VKNHLLMSHT AFQRDLADAK 600
    TITDFANAVK SPERLRLLYL LTVADISAVG PHIWNSWKNQ LLTSLYEACS 650
    QCLLEGPTGH GAGRQQRIEN RQNDVSEKLD WDNDLFHALV KRLPDDYWFS 700
    ERTDVIAANM QQIIDTDSKG QSISVRGHEM PAYDATMISL YAIDHPGFFY 750
    RISGAIHATG GNILDARIHT TRDGMAMDNL LVQNSQGGMI KSGEHLNRMM 800
    QAIEDAATSH IRSSNKLAAL RPPLFWRGKA FHVEPLVFID NQASDRFTVI 850
    EVNAQDRPAL LHDLGCALFN ARLTISSAHI ATYGERAVDV FYVSDLFSHK 900
    ITNQNRLKAI EKRLLAAADA ARISEK 926
    Length:926
    Mass (Da):104,393
    Last modified:February 1, 2005 - v1
    Checksum:iA854FC1B043AD4BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008692 Genomic DNA. Translation: AAV89390.1.
    RefSeqiYP_162501.1. NC_006526.2.

    Genome annotation databases

    EnsemblBacteriaiAAV89390; AAV89390; ZMO0766.
    GeneIDi3188782.
    KEGGizmo:ZMO0766.
    PATRICi32566842. VBIZymMob102260_0725.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008692 Genomic DNA. Translation: AAV89390.1 .
    RefSeqi YP_162501.1. NC_006526.2.

    3D structure databases

    ProteinModelPortali Q5NPH0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 264203.ZMO0766.

    Proteomic databases

    PRIDEi Q5NPH0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAV89390 ; AAV89390 ; ZMO0766 .
    GeneIDi 3188782.
    KEGGi zmo:ZMO0766.
    PATRICi 32566842. VBIZymMob102260_0725.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261779.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 31821 / ZM4 / CP4.

    Entry informationi

    Entry nameiGLND_ZYMMO
    AccessioniPrimary (citable) accession number: Q5NPH0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3