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Q5NPH0

- GLND_ZYMMO

UniProt

Q5NPH0 - GLND_ZYMMO

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, ZMO0766
Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:ZMO0766
OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Taxonomic identifieri264203 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
ProteomesiUP000001173: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 926926Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_0000231700Add
BLAST

Proteomic databases

PRIDEiQ5NPH0.

Interactioni

Protein-protein interaction databases

STRINGi264203.ZMO0766.

Structurei

3D structure databases

ProteinModelPortaliQ5NPH0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini523 – 61795HDAdd
BLAST
Domaini737 – 81478ACT 1Add
BLAST
Domaini849 – 92678ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 379379UridylyltransferaseUniRule annotationAdd
BLAST
Regioni380 – 736357Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5NPH0-1 [UniParc]FASTAAdd to Basket

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MPVSFLSLAS QPQIIDYPAI KEEIDSIKKV AAGDPVSEYR AVSLVLKQTV    50
LKGREVIAEA LTRRPHQGRI AALSYAYLTD QIIQLALYAM VGDDEKATDN 100
LVILAVGGYG RGEMALHSDV DIAFLSRRSP RKAQKKLIES LISLLWDVGL 150
RVSQSHRSLS DMVKMAKKDI TIRTALLESR YLVGDKALFE EASTRFIQKV 200
VAGSGRDFVA AKLLEWDERH LARGDSRYLV EPHLKEGKGG LRDLQSLFWI 250
AKYLYLEKEA RHTVLTSPIL TDYALVKADL IAGHEAKRFR RCENFLWAVR 300
CHLHILVKRP EERLNFDVQR SLAEKMGYRS KSGKSAVERF MHHYFLVTKM 350
VGNLAALFID ALKVNHYLKP KSRRSGASKQ IDGFPVIQGE IVLDDDFFFR 400
HNPAALITLF AVAYRHRLDI HPLTLRQAGR DARLIDEALQ NNPVCNAAFL 450
TLFTLPYNPA PILKIMNNTG VLGRFIPDFG RIVAQMQFDM YHHYTVDEHA 500
IRALDILWQI ENNKLEDLYP LGSRLFKQLN ARLVLYMALF LHDIAKGREG 550
SHSALGAEIA LRLCPRFGLT AAETKLVAWL VKNHLLMSHT AFQRDLADAK 600
TITDFANAVK SPERLRLLYL LTVADISAVG PHIWNSWKNQ LLTSLYEACS 650
QCLLEGPTGH GAGRQQRIEN RQNDVSEKLD WDNDLFHALV KRLPDDYWFS 700
ERTDVIAANM QQIIDTDSKG QSISVRGHEM PAYDATMISL YAIDHPGFFY 750
RISGAIHATG GNILDARIHT TRDGMAMDNL LVQNSQGGMI KSGEHLNRMM 800
QAIEDAATSH IRSSNKLAAL RPPLFWRGKA FHVEPLVFID NQASDRFTVI 850
EVNAQDRPAL LHDLGCALFN ARLTISSAHI ATYGERAVDV FYVSDLFSHK 900
ITNQNRLKAI EKRLLAAADA ARISEK 926
Length:926
Mass (Da):104,393
Last modified:February 1, 2005 - v1
Checksum:iA854FC1B043AD4BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008692 Genomic DNA. Translation: AAV89390.1.
RefSeqiYP_162501.1. NC_006526.2.

Genome annotation databases

EnsemblBacteriaiAAV89390; AAV89390; ZMO0766.
GeneIDi3188782.
KEGGizmo:ZMO0766.
PATRICi32566842. VBIZymMob102260_0725.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008692 Genomic DNA. Translation: AAV89390.1 .
RefSeqi YP_162501.1. NC_006526.2.

3D structure databases

ProteinModelPortali Q5NPH0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264203.ZMO0766.

Proteomic databases

PRIDEi Q5NPH0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV89390 ; AAV89390 ; ZMO0766 .
GeneIDi 3188782.
KEGGi zmo:ZMO0766.
PATRICi 32566842. VBIZymMob102260_0725.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31821 / ZM4 / CP4.

Entry informationi

Entry nameiGLND_ZYMMO
AccessioniPrimary (citable) accession number: Q5NPH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: February 1, 2005
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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