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Q5NP36

- SYE1_ZYMMO

UniProt

Q5NP36 - SYE1_ZYMMO

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Protein

Glutamate--tRNA ligase 1

Gene
gltX1, ZMO0900
Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei242 – 2421ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 1 (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name:
GluRS 1
Gene namesi
Name:gltX1
Ordered Locus Names:ZMO0900
OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Taxonomic identifieri264203 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
ProteomesiUP000001173: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Glutamate--tRNA ligase 1UniRule annotationPRO_0000119710Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi264203.ZMO0900.

Structurei

3D structure databases

ProteinModelPortaliQ5NP36.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi8 – 1811"HIGH" regionUniRule annotationAdd
BLAST
Motifi239 – 2435"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252721.
KOiK01885.
OMAiKEDIAKP.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5NP36-1 [UniParc]FASTAAdd to Basket

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MITTRFAPSP TGFLHVGNIR TALINWLYAR HHHGRFVLRI DDTDSERSTE    50
EYTQAIRADL AWLNLNPDVE YSQSKRSELY EIRFESLKAK GRIYPCYETP 100
QELDLKRRIA IGRGLPPIYD RAALNLTIEE RSELEEKGIK PHWRFRLDHD 150
HAIKWNDLIR GEQRFDPKLL SDPVIRRADG SWLYLLPSVI DDIDMEITTI 200
VRGEDHVSNT ATQIQMFQAL EAELPEFAHM ALLTGGDAKI SKRFGADSVA 250
DFKADHIEPI ALEALLARIG TSDPVEVLTD LAPLVENFDF SHFSRAPARF 300
DMAELKHLNA RILHITPYEQ VADRLPEAMD AHSWEVIRPN IDDLSEAAFW 350
WSVVKEDIAK PDLSAEDKAY LVEAMAIAKT LDWSSNPWKE WIDILKKQSG 400
RKGKALFLPL RLALTGQSHG PDMAGLLSLI GHEKAIKRLE QAVK 444
Length:444
Mass (Da):50,635
Last modified:February 1, 2005 - v1
Checksum:i68BB6E2DFFE95C8B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008692 Genomic DNA. Translation: AAV89524.1.
RefSeqiYP_162635.1. NC_006526.2.

Genome annotation databases

EnsemblBacteriaiAAV89524; AAV89524; ZMO0900.
GeneIDi3188145.
KEGGizmo:ZMO0900.
PATRICi32567088. VBIZymMob102260_0847.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008692 Genomic DNA. Translation: AAV89524.1 .
RefSeqi YP_162635.1. NC_006526.2.

3D structure databases

ProteinModelPortali Q5NP36.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264203.ZMO0900.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV89524 ; AAV89524 ; ZMO0900 .
GeneIDi 3188145.
KEGGi zmo:ZMO0900.
PATRICi 32567088. VBIZymMob102260_0847.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252721.
KOi K01885.
OMAi KEDIAKP.
OrthoDBi EOG6DRPF7.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31821 / ZM4 / CP4.

Entry informationi

Entry nameiSYE1_ZYMMO
AccessioniPrimary (citable) accession number: Q5NP36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: February 1, 2005
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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