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Protein

Alkaline phosphatase PhoD

Gene

phoD

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Alkaline phosphatase with broad substrate specificity. Has phosphatase activity towards nucleotide and sugar phosphates with a preference to nucleotide phosphates. Has no phosphodiesterase activity.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi68 – 681Zinc 1By similarity
Active sitei107 – 1071Phosphothreonine intermediateBy similarity
Metal bindingi107 – 1071Zinc 1By similarity
Binding sitei128 – 1281SubstrateBy similarity
Metal bindingi318 – 3181Zinc 2By similarity
Metal bindingi322 – 3221Zinc 2; via tele nitrogenBy similarity
Metal bindingi363 – 3631Zinc 1By similarity
Metal bindingi364 – 3641Zinc 1; via tele nitrogenBy similarity
Metal bindingi508 – 5081Zinc 2; via tele nitrogenBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase PhoD1 PublicationImported (EC:3.1.3.1Imported)
Short name:
ALPI1 Publication
Alternative name(s):
Type I phosphodiesterase/nucleotide pyrophosphataseImported
Gene namesi
Name:phoDImported
Ordered Locus Names:ZMO0938
OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Taxonomic identifieri264203 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
ProteomesiUP000001173 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 576544Alkaline phosphatase PhoDSequence AnalysisPRO_5000559515Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi108 ↔ 144By similarity
Disulfide bondi248 ↔ 332By similarity
Disulfide bondi562 ↔ 573By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi555217.Zmob_0365.

Structurei

3D structure databases

ProteinModelPortaliQ5NNZ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni188 – 1903Substrate bindingBy similarity

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000288350.
KOiK01077.
OMAiHANTTTC.
OrthoDBiEOG6PCPWQ.

Family and domain databases

Gene3Di3.40.720.10. 2 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR026263. Alkaline_phosphatase_prok.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
SUPFAMiSSF53649. SSF53649. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5NNZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSLLHHSFL KTVFSSLAIA IVTSSLSSVT IAATHPLDNH PKGEIAASSE
60 70 80 90 100
TAHNPWSGTR LIVAISVDQF SSDLFSEYRG RFRSGMKQLQ NGVVYPMAYH
110 120 130 140 150
SHAATETCPG HSVLLTGDHP ARTGIIANNW YDFSVKRADK KVYCSEDPSL
160 170 180 190 200
SADPQNYQPS VHYLKVPTLG DRMKKANPHS RVISVAGKDR AAIMMGGHMT
210 220 230 240 250
DQIWFWSDNA YKTLADHKGE MPVTVKTVNE QVTRFMQQDE APVMPSVCAD
260 270 280 290 300
HASALKIGNN RIIGLAPASR KAGDFKTFRV TPDYDRTTTD IAIGLIDELK
310 320 330 340 350
LGHGNAPDLL TVSLSATDAV GHAYGTEGAE MCSQMAGLDD NIARIIAALD
360 370 380 390 400
SNGVPYVLVL TADHGGQDVP ERAKLRGVET AQRVDPALSP DQLSLRLAER
410 420 430 440 450
FQLSHNQPLF FANEPQGDWY INRNLPEQTK AQLIQAAKSE LSNHPQVAAV
460 470 480 490 500
FTASELTHIP YPTRSPELWN LAERAKASFD PLRSGDLIVL LKPRVTPIAK
510 520 530 540 550
PVSYVATHGS AWDYDRRVPI IFYTPHASGF EQPMPVETVD IMPSLAALLQ
560 570
IPLRKGEVDG RCLDLDPTEA TTCPVK
Length:576
Mass (Da):63,011
Last modified:February 1, 2005 - v1
Checksum:iFE69A39214F67F4C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti158 – 1581Q → K in AAA74034 (PubMed:7875572).Curated
Sequence conflicti223 – 2231V → A in AAA74034 (PubMed:7875572).Curated
Sequence conflicti235 – 2351F → L in AAA74034 (PubMed:7875572).Curated
Sequence conflicti272 – 2721A → T in AAA74034 (PubMed:7875572).Curated
Sequence conflicti396 – 3961R → K in AAA74034 (PubMed:7875572).Curated
Sequence conflicti461 – 4611Y → H in AAA74034 (PubMed:7875572).Curated
Sequence conflicti476 – 4761K → E in AAA74034 (PubMed:7875572).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36230 Genomic DNA. Translation: AAA74034.1.
AE008692 Genomic DNA. Translation: AAV89562.1.
RefSeqiWP_011240797.1. NC_006526.2.
YP_162673.1. NC_006526.2.

Genome annotation databases

EnsemblBacteriaiAAV89562; AAV89562; ZMO0938.
KEGGizmo:ZMO0938.
PATRICi32567162. VBIZymMob102260_0883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36230 Genomic DNA. Translation: AAA74034.1.
AE008692 Genomic DNA. Translation: AAV89562.1.
RefSeqiWP_011240797.1. NC_006526.2.
YP_162673.1. NC_006526.2.

3D structure databases

ProteinModelPortaliQ5NNZ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi555217.Zmob_0365.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV89562; AAV89562; ZMO0938.
KEGGizmo:ZMO0938.
PATRICi32567162. VBIZymMob102260_0883.

Phylogenomic databases

HOGENOMiHOG000288350.
KOiK01077.
OMAiHANTTTC.
OrthoDBiEOG6PCPWQ.

Family and domain databases

Gene3Di3.40.720.10. 2 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR026263. Alkaline_phosphatase_prok.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
SUPFAMiSSF53649. SSF53649. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing and characterization of the alkaline phosphatase gene (phoD) from Zymomonas mobilis."
    Gomez P.F., Ingram L.O.
    FEMS Microbiol. Lett. 125:237-245(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE NAME, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
    Strain: ATCC 31821 / ZM4 / CP4Imported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31821 / ZM4 / CP4.

Entry informationi

Entry nameiALPH_ZYMMO
AccessioniPrimary (citable) accession number: Q5NNZ8
Secondary accession number(s): Q60113
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: February 1, 2005
Last modified: June 24, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.