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Protein

Alkaline phosphatase PhoD

Gene

phoD

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Alkaline phosphatase with broad substrate specificity. Has phosphatase activity towards nucleotide and sugar phosphates with a preference to nucleotide phosphates. Has no phosphodiesterase activity.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi68Zinc 1By similarity1
Active sitei107Phosphothreonine intermediateBy similarity1
Metal bindingi107Zinc 1By similarity1
Binding sitei128SubstrateBy similarity1
Metal bindingi318Zinc 2By similarity1
Metal bindingi322Zinc 2; via tele nitrogenBy similarity1
Metal bindingi363Zinc 1By similarity1
Metal bindingi364Zinc 1; via tele nitrogenBy similarity1
Metal bindingi508Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase PhoD1 PublicationImported (EC:3.1.3.1Imported)
Short name:
ALPI1 Publication
Alternative name(s):
Type I phosphodiesterase/nucleotide pyrophosphataseImported
Gene namesi
Name:phoDImported
Ordered Locus Names:ZMO0938
OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Taxonomic identifieri264203 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
Proteomesi
  • UP000001173 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_500055951533 – 576Alkaline phosphatase PhoDSequence analysisAdd BLAST544

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi108 ↔ 144By similarity
Disulfide bondi248 ↔ 332By similarity
Disulfide bondi562 ↔ 573By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ5NNZ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni188 – 190Substrate bindingBy similarity3

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000288350.
KOiK01077.
OMAiGTEGAEM.

Family and domain databases

Gene3Di3.40.720.10. 2 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR026263. Alkaline_phosphatase_prok.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
SUPFAMiSSF53649. SSF53649. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5NNZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSLLHHSFL KTVFSSLAIA IVTSSLSSVT IAATHPLDNH PKGEIAASSE
60 70 80 90 100
TAHNPWSGTR LIVAISVDQF SSDLFSEYRG RFRSGMKQLQ NGVVYPMAYH
110 120 130 140 150
SHAATETCPG HSVLLTGDHP ARTGIIANNW YDFSVKRADK KVYCSEDPSL
160 170 180 190 200
SADPQNYQPS VHYLKVPTLG DRMKKANPHS RVISVAGKDR AAIMMGGHMT
210 220 230 240 250
DQIWFWSDNA YKTLADHKGE MPVTVKTVNE QVTRFMQQDE APVMPSVCAD
260 270 280 290 300
HASALKIGNN RIIGLAPASR KAGDFKTFRV TPDYDRTTTD IAIGLIDELK
310 320 330 340 350
LGHGNAPDLL TVSLSATDAV GHAYGTEGAE MCSQMAGLDD NIARIIAALD
360 370 380 390 400
SNGVPYVLVL TADHGGQDVP ERAKLRGVET AQRVDPALSP DQLSLRLAER
410 420 430 440 450
FQLSHNQPLF FANEPQGDWY INRNLPEQTK AQLIQAAKSE LSNHPQVAAV
460 470 480 490 500
FTASELTHIP YPTRSPELWN LAERAKASFD PLRSGDLIVL LKPRVTPIAK
510 520 530 540 550
PVSYVATHGS AWDYDRRVPI IFYTPHASGF EQPMPVETVD IMPSLAALLQ
560 570
IPLRKGEVDG RCLDLDPTEA TTCPVK
Length:576
Mass (Da):63,011
Last modified:February 1, 2005 - v1
Checksum:iFE69A39214F67F4C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti158Q → K in AAA74034 (PubMed:7875572).Curated1
Sequence conflicti223V → A in AAA74034 (PubMed:7875572).Curated1
Sequence conflicti235F → L in AAA74034 (PubMed:7875572).Curated1
Sequence conflicti272A → T in AAA74034 (PubMed:7875572).Curated1
Sequence conflicti396R → K in AAA74034 (PubMed:7875572).Curated1
Sequence conflicti461Y → H in AAA74034 (PubMed:7875572).Curated1
Sequence conflicti476K → E in AAA74034 (PubMed:7875572).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36230 Genomic DNA. Translation: AAA74034.1.
AE008692 Genomic DNA. Translation: AAV89562.1.
RefSeqiWP_011240797.1. NC_006526.2.

Genome annotation databases

EnsemblBacteriaiAAV89562; AAV89562; ZMO0938.
KEGGizmo:ZMO0938.
PATRICi32567162. VBIZymMob102260_0883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36230 Genomic DNA. Translation: AAA74034.1.
AE008692 Genomic DNA. Translation: AAV89562.1.
RefSeqiWP_011240797.1. NC_006526.2.

3D structure databases

ProteinModelPortaliQ5NNZ8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV89562; AAV89562; ZMO0938.
KEGGizmo:ZMO0938.
PATRICi32567162. VBIZymMob102260_0883.

Phylogenomic databases

HOGENOMiHOG000288350.
KOiK01077.
OMAiGTEGAEM.

Family and domain databases

Gene3Di3.40.720.10. 2 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR026263. Alkaline_phosphatase_prok.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
SUPFAMiSSF53649. SSF53649. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiALPH_ZYMMO
AccessioniPrimary (citable) accession number: Q5NNZ8
Secondary accession number(s): Q60113
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: February 1, 2005
Last modified: September 7, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.