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Q5NNZ8

- ALPH_ZYMMO

UniProt

Q5NNZ8 - ALPH_ZYMMO

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Protein
Alkaline phosphatase PhoD
Gene
phoD, ZMO0938
Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Alkaline phosphatase with broad substrate specificity. Has phosphatase activity towards nucleotide and sugar phosphates with a preference to nucleotide phosphates. Has no phosphodiesterase activity.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Binds 2 zinc ions By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi68 – 681Zinc 1 By similarity
Active sitei107 – 1071Phosphothreonine intermediate By similarity
Metal bindingi107 – 1071Zinc 1 By similarity
Binding sitei128 – 1281Substrate By similarity
Metal bindingi318 – 3181Zinc 2 By similarity
Metal bindingi322 – 3221Zinc 2; via tele nitrogen By similarity
Metal bindingi363 – 3631Zinc 1 By similarity
Metal bindingi364 – 3641Zinc 1; via tele nitrogen By similarity
Metal bindingi508 – 5081Zinc 2; via tele nitrogen By similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase PhoD (EC:3.1.3.1)
    Short name:
    ALPI
    Alternative name(s):
    Type I phosphodiesterase/nucleotide pyrophosphatase
    Gene namesi
    Name:phoD
    Ordered Locus Names:ZMO0938
    OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
    Taxonomic identifieri264203 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
    ProteomesiUP000001173: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232 Reviewed prediction
    Add
    BLAST
    Chaini33 – 576544Alkaline phosphatase PhoD
    PRO_5000559515Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi108 ↔ 144 By similarity
    Disulfide bondi248 ↔ 332 By similarity
    Disulfide bondi562 ↔ 573 By similarity

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi264203.ZMO0938.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5NNZ8.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni188 – 1903Substrate binding By similarity

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000288350.
    KOiK01077.
    OMAiCAGHSTI.
    OrthoDBiEOG6PCPWQ.

    Family and domain databases

    Gene3Di3.40.720.10. 2 hits.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR026263. Alkaline_phosphatase_prok.
    IPR002591. Phosphodiest/P_Trfase.
    [Graphical view]
    PfamiPF01663. Phosphodiest. 1 hit.
    [Graphical view]
    PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
    SUPFAMiSSF53649. SSF53649. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5NNZ8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSLLHHSFL KTVFSSLAIA IVTSSLSSVT IAATHPLDNH PKGEIAASSE    50
    TAHNPWSGTR LIVAISVDQF SSDLFSEYRG RFRSGMKQLQ NGVVYPMAYH 100
    SHAATETCPG HSVLLTGDHP ARTGIIANNW YDFSVKRADK KVYCSEDPSL 150
    SADPQNYQPS VHYLKVPTLG DRMKKANPHS RVISVAGKDR AAIMMGGHMT 200
    DQIWFWSDNA YKTLADHKGE MPVTVKTVNE QVTRFMQQDE APVMPSVCAD 250
    HASALKIGNN RIIGLAPASR KAGDFKTFRV TPDYDRTTTD IAIGLIDELK 300
    LGHGNAPDLL TVSLSATDAV GHAYGTEGAE MCSQMAGLDD NIARIIAALD 350
    SNGVPYVLVL TADHGGQDVP ERAKLRGVET AQRVDPALSP DQLSLRLAER 400
    FQLSHNQPLF FANEPQGDWY INRNLPEQTK AQLIQAAKSE LSNHPQVAAV 450
    FTASELTHIP YPTRSPELWN LAERAKASFD PLRSGDLIVL LKPRVTPIAK 500
    PVSYVATHGS AWDYDRRVPI IFYTPHASGF EQPMPVETVD IMPSLAALLQ 550
    IPLRKGEVDG RCLDLDPTEA TTCPVK 576
    Length:576
    Mass (Da):63,011
    Last modified:February 1, 2005 - v1
    Checksum:iFE69A39214F67F4C
    GO

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti158 – 1581Q → K in AAA74034. 1 Publication
    Sequence conflicti223 – 2231V → A in AAA74034. 1 Publication
    Sequence conflicti235 – 2351F → L in AAA74034. 1 Publication
    Sequence conflicti272 – 2721A → T in AAA74034. 1 Publication
    Sequence conflicti396 – 3961R → K in AAA74034. 1 Publication
    Sequence conflicti461 – 4611Y → H in AAA74034. 1 Publication
    Sequence conflicti476 – 4761K → E in AAA74034. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36230 Genomic DNA. Translation: AAA74034.1.
    AE008692 Genomic DNA. Translation: AAV89562.1.
    RefSeqiYP_162673.1. NC_006526.2.

    Genome annotation databases

    EnsemblBacteriaiAAV89562; AAV89562; ZMO0938.
    GeneIDi3188170.
    KEGGizmo:ZMO0938.
    PATRICi32567162. VBIZymMob102260_0883.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36230 Genomic DNA. Translation: AAA74034.1 .
    AE008692 Genomic DNA. Translation: AAV89562.1 .
    RefSeqi YP_162673.1. NC_006526.2.

    3D structure databases

    ProteinModelPortali Q5NNZ8.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 264203.ZMO0938.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAV89562 ; AAV89562 ; ZMO0938 .
    GeneIDi 3188170.
    KEGGi zmo:ZMO0938.
    PATRICi 32567162. VBIZymMob102260_0883.

    Phylogenomic databases

    HOGENOMi HOG000288350.
    KOi K01077.
    OMAi CAGHSTI.
    OrthoDBi EOG6PCPWQ.

    Family and domain databases

    Gene3Di 3.40.720.10. 2 hits.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR026263. Alkaline_phosphatase_prok.
    IPR002591. Phosphodiest/P_Trfase.
    [Graphical view ]
    Pfami PF01663. Phosphodiest. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF031924. Pi-irrepressible_AP. 1 hit.
    SUPFAMi SSF53649. SSF53649. 2 hits.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning, sequencing and characterization of the alkaline phosphatase gene (phoD) from Zymomonas mobilis."
      Gomez P.F., Ingram L.O.
      FEMS Microbiol. Lett. 125:237-245(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE NAME, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
      Strain: ATCC 31821 / ZM4 / CP4.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 31821 / ZM4 / CP4.

    Entry informationi

    Entry nameiALPH_ZYMMO
    AccessioniPrimary (citable) accession number: Q5NNZ8
    Secondary accession number(s): Q60113
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 19, 2014
    Last sequence update: February 1, 2005
    Last modified: June 11, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    External Data

    Dasty 3

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