Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q5NNZ8

- ALPH_ZYMMO

UniProt

Q5NNZ8 - ALPH_ZYMMO

Protein

Alkaline phosphatase PhoD

Gene

phoD

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (01 Feb 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Alkaline phosphatase with broad substrate specificity. Has phosphatase activity towards nucleotide and sugar phosphates with a preference to nucleotide phosphates. Has no phosphodiesterase activity.1 Publication

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

    Cofactori

    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi68 – 681Zinc 1By similarity
    Active sitei107 – 1071Phosphothreonine intermediateBy similarity
    Metal bindingi107 – 1071Zinc 1By similarity
    Binding sitei128 – 1281SubstrateBy similarity
    Metal bindingi318 – 3181Zinc 2By similarity
    Metal bindingi322 – 3221Zinc 2; via tele nitrogenBy similarity
    Metal bindingi363 – 3631Zinc 1By similarity
    Metal bindingi364 – 3641Zinc 1; via tele nitrogenBy similarity
    Metal bindingi508 – 5081Zinc 2; via tele nitrogenBy similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase PhoDImported1 Publication (EC:3.1.3.1Imported)
    Short name:
    ALPI1 Publication
    Alternative name(s):
    Type I phosphodiesterase/nucleotide pyrophosphataseImported
    Gene namesi
    Name:phoDImported
    Ordered Locus Names:ZMO0938
    OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
    Taxonomic identifieri264203 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
    ProteomesiUP000001173: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Sequence AnalysisAdd
    BLAST
    Chaini33 – 576544Alkaline phosphatase PhoDSequence AnalysisPRO_5000559515Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi108 ↔ 144By similarity
    Disulfide bondi248 ↔ 332By similarity
    Disulfide bondi562 ↔ 573By similarity

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi264203.ZMO0938.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5NNZ8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni188 – 1903Substrate bindingBy similarity

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000288350.
    KOiK01077.
    OMAiCAGHSTI.
    OrthoDBiEOG6PCPWQ.

    Family and domain databases

    Gene3Di3.40.720.10. 2 hits.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR026263. Alkaline_phosphatase_prok.
    IPR002591. Phosphodiest/P_Trfase.
    [Graphical view]
    PfamiPF01663. Phosphodiest. 1 hit.
    [Graphical view]
    PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
    SUPFAMiSSF53649. SSF53649. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5NNZ8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSLLHHSFL KTVFSSLAIA IVTSSLSSVT IAATHPLDNH PKGEIAASSE    50
    TAHNPWSGTR LIVAISVDQF SSDLFSEYRG RFRSGMKQLQ NGVVYPMAYH 100
    SHAATETCPG HSVLLTGDHP ARTGIIANNW YDFSVKRADK KVYCSEDPSL 150
    SADPQNYQPS VHYLKVPTLG DRMKKANPHS RVISVAGKDR AAIMMGGHMT 200
    DQIWFWSDNA YKTLADHKGE MPVTVKTVNE QVTRFMQQDE APVMPSVCAD 250
    HASALKIGNN RIIGLAPASR KAGDFKTFRV TPDYDRTTTD IAIGLIDELK 300
    LGHGNAPDLL TVSLSATDAV GHAYGTEGAE MCSQMAGLDD NIARIIAALD 350
    SNGVPYVLVL TADHGGQDVP ERAKLRGVET AQRVDPALSP DQLSLRLAER 400
    FQLSHNQPLF FANEPQGDWY INRNLPEQTK AQLIQAAKSE LSNHPQVAAV 450
    FTASELTHIP YPTRSPELWN LAERAKASFD PLRSGDLIVL LKPRVTPIAK 500
    PVSYVATHGS AWDYDRRVPI IFYTPHASGF EQPMPVETVD IMPSLAALLQ 550
    IPLRKGEVDG RCLDLDPTEA TTCPVK 576
    Length:576
    Mass (Da):63,011
    Last modified:February 1, 2005 - v1
    Checksum:iFE69A39214F67F4C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti158 – 1581Q → K in AAA74034. (PubMed:7875572)Curated
    Sequence conflicti223 – 2231V → A in AAA74034. (PubMed:7875572)Curated
    Sequence conflicti235 – 2351F → L in AAA74034. (PubMed:7875572)Curated
    Sequence conflicti272 – 2721A → T in AAA74034. (PubMed:7875572)Curated
    Sequence conflicti396 – 3961R → K in AAA74034. (PubMed:7875572)Curated
    Sequence conflicti461 – 4611Y → H in AAA74034. (PubMed:7875572)Curated
    Sequence conflicti476 – 4761K → E in AAA74034. (PubMed:7875572)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36230 Genomic DNA. Translation: AAA74034.1.
    AE008692 Genomic DNA. Translation: AAV89562.1.
    RefSeqiYP_162673.1. NC_006526.2.

    Genome annotation databases

    EnsemblBacteriaiAAV89562; AAV89562; ZMO0938.
    GeneIDi3188170.
    KEGGizmo:ZMO0938.
    PATRICi32567162. VBIZymMob102260_0883.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36230 Genomic DNA. Translation: AAA74034.1 .
    AE008692 Genomic DNA. Translation: AAV89562.1 .
    RefSeqi YP_162673.1. NC_006526.2.

    3D structure databases

    ProteinModelPortali Q5NNZ8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 264203.ZMO0938.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAV89562 ; AAV89562 ; ZMO0938 .
    GeneIDi 3188170.
    KEGGi zmo:ZMO0938.
    PATRICi 32567162. VBIZymMob102260_0883.

    Phylogenomic databases

    HOGENOMi HOG000288350.
    KOi K01077.
    OMAi CAGHSTI.
    OrthoDBi EOG6PCPWQ.

    Family and domain databases

    Gene3Di 3.40.720.10. 2 hits.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR026263. Alkaline_phosphatase_prok.
    IPR002591. Phosphodiest/P_Trfase.
    [Graphical view ]
    Pfami PF01663. Phosphodiest. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF031924. Pi-irrepressible_AP. 1 hit.
    SUPFAMi SSF53649. SSF53649. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing and characterization of the alkaline phosphatase gene (phoD) from Zymomonas mobilis."
      Gomez P.F., Ingram L.O.
      FEMS Microbiol. Lett. 125:237-245(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE NAME, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
      Strain: ATCC 31821 / ZM4 / CP4Imported.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 31821 / ZM4 / CP4.

    Entry informationi

    Entry nameiALPH_ZYMMO
    AccessioniPrimary (citable) accession number: Q5NNZ8
    Secondary accession number(s): Q60113
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 19, 2014
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    External Data

    Dasty 3