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Q5NNZ8 (ALPH_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Alkaline phosphatase PhoD

Short name=ALPI
EC=3.1.3.1
Alternative name(s):
Type I phosphodiesterase/nucleotide pyrophosphatase
Gene names
Name:phoD
Ordered Locus Names:ZMO0938
OrganismZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) [Complete proteome] [HAMAP]
Taxonomic identifier264203 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Alkaline phosphatase with broad substrate specificity. Has phosphatase activity towards nucleotide and sugar phosphates with a preference to nucleotide phosphates. Has no phosphodiesterase activity. Ref.1

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate. Ref.1

Cofactor

Binds 2 zinc ions By similarity.

Subunit structure

Monomer. Ref.1

Ontologies

Keywords
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   PTMDisulfide bond
Phosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functionalkaline phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 576544Alkaline phosphatase PhoD
PRO_5000559515

Regions

Region188 – 1903Substrate binding By similarity

Sites

Active site1071Phosphothreonine intermediate By similarity
Metal binding681Zinc 1 By similarity
Metal binding1071Zinc 1 By similarity
Metal binding3181Zinc 2 By similarity
Metal binding3221Zinc 2; via tele nitrogen By similarity
Metal binding3631Zinc 1 By similarity
Metal binding3641Zinc 1; via tele nitrogen By similarity
Metal binding5081Zinc 2; via tele nitrogen By similarity
Binding site1281Substrate By similarity

Amino acid modifications

Disulfide bond108 ↔ 144 By similarity
Disulfide bond248 ↔ 332 By similarity
Disulfide bond562 ↔ 573 By similarity

Experimental info

Sequence conflict1581Q → K in AAA74034. Ref.1
Sequence conflict2231V → A in AAA74034. Ref.1
Sequence conflict2351F → L in AAA74034. Ref.1
Sequence conflict2721A → T in AAA74034. Ref.1
Sequence conflict3961R → K in AAA74034. Ref.1
Sequence conflict4611Y → H in AAA74034. Ref.1
Sequence conflict4761K → E in AAA74034. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q5NNZ8 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: FE69A39214F67F4C

FASTA57663,011
        10         20         30         40         50         60 
MNSLLHHSFL KTVFSSLAIA IVTSSLSSVT IAATHPLDNH PKGEIAASSE TAHNPWSGTR 

        70         80         90        100        110        120 
LIVAISVDQF SSDLFSEYRG RFRSGMKQLQ NGVVYPMAYH SHAATETCPG HSVLLTGDHP 

       130        140        150        160        170        180 
ARTGIIANNW YDFSVKRADK KVYCSEDPSL SADPQNYQPS VHYLKVPTLG DRMKKANPHS 

       190        200        210        220        230        240 
RVISVAGKDR AAIMMGGHMT DQIWFWSDNA YKTLADHKGE MPVTVKTVNE QVTRFMQQDE 

       250        260        270        280        290        300 
APVMPSVCAD HASALKIGNN RIIGLAPASR KAGDFKTFRV TPDYDRTTTD IAIGLIDELK 

       310        320        330        340        350        360 
LGHGNAPDLL TVSLSATDAV GHAYGTEGAE MCSQMAGLDD NIARIIAALD SNGVPYVLVL 

       370        380        390        400        410        420 
TADHGGQDVP ERAKLRGVET AQRVDPALSP DQLSLRLAER FQLSHNQPLF FANEPQGDWY 

       430        440        450        460        470        480 
INRNLPEQTK AQLIQAAKSE LSNHPQVAAV FTASELTHIP YPTRSPELWN LAERAKASFD 

       490        500        510        520        530        540 
PLRSGDLIVL LKPRVTPIAK PVSYVATHGS AWDYDRRVPI IFYTPHASGF EQPMPVETVD 

       550        560        570 
IMPSLAALLQ IPLRKGEVDG RCLDLDPTEA TTCPVK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing and characterization of the alkaline phosphatase gene (phoD) from Zymomonas mobilis."
Gomez P.F., Ingram L.O.
FEMS Microbiol. Lett. 125:237-245(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE NAME, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
Strain: ATCC 31821 / ZM4 / CP4.
[2]"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4."
Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. expand/collapse author list , Kang H.L., Lee S.Y., Lee K.J., Kang H.S.
Nat. Biotechnol. 23:63-68(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L36230 Genomic DNA. Translation: AAA74034.1.
AE008692 Genomic DNA. Translation: AAV89562.1.
RefSeqYP_162673.1. NC_006526.2.

3D structure databases

ProteinModelPortalQ5NNZ8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264203.ZMO0938.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV89562; AAV89562; ZMO0938.
GeneID3188170.
KEGGzmo:ZMO0938.
PATRIC32567162. VBIZymMob102260_0883.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000288350.
KOK01077.
OMACAGHSTI.
OrthoDBEOG6PCPWQ.

Family and domain databases

Gene3D3.40.720.10. 2 hits.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR026263. Alkaline_phosphatase_prok.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PfamPF01663. Phosphodiest. 1 hit.
[Graphical view]
PIRSFPIRSF031924. Pi-irrepressible_AP. 1 hit.
SUPFAMSSF53649. SSF53649. 2 hits.
ProtoNetSearch...

Entry information

Entry nameALPH_ZYMMO
AccessionPrimary (citable) accession number: Q5NNZ8
Secondary accession number(s): Q60113
Entry history
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: February 1, 2005
Last modified: June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program