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Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

Cofactori

Zn2+UniRule annotation, Mg2+UniRule annotation, Co2+UniRule annotationNote: Binds 1 divalent metal cation per subunit. Can use ions such as Zn(2+), Mg(2+) or Co2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei132 – 1321SubstrateUniRule annotation
Metal bindingi163 – 1631Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi208 – 2081Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi263 – 2631Divalent metal cation; shared with dimeric partnerUniRule annotation
Binding sitei271 – 2711SubstrateUniRule annotation
Binding sitei280 – 2801SubstrateUniRule annotation
Binding sitei289 – 2891SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridoxine biosynthesis

Keywords - Ligandi

Cobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
Gene namesi
Name:pdxAUniRule annotation
Ordered Locus Names:ZMO1313
OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Taxonomic identifieri264203 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
ProteomesiUP000001173 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3353354-hydroxythreonine-4-phosphate dehydrogenasePRO_1000051526Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi264203.ZMO1313.

Structurei

3D structure databases

ProteinModelPortaliQ5NMX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1995.
HOGENOMiHOG000221592.
KOiK00097.
OMAiDTLFQDK.
OrthoDBiEOG6GN6ZC.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00557. pdxA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5NMX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPLAVTLGD PSGIGPEIVA KAWSRRKSDQ IMPFFAIGSA ASIQAVSSIP
60 70 80 90 100
VVAITDPNEA ISIFDQALPV WDIPSKETIT PGKPNKAGAE VAFAALEKGV
110 120 130 140 150
ALVKQGQASA LVTAPVSKAE LYQVGFTFPG QTEFVANRCG IAADDAVMML
160 170 180 190 200
AGPDLRTVPL TIHIPYRDVL EQLTPKLIIS RARVTVEDLK RNFAIPSPRL
210 220 230 240 250
VVAGLNPHAG ENGTIGREEI DSIEPAIRQL QAENIDIKGP FAADTLFSPR
260 270 280 290 300
ARATYDVALC PTHDQALIPI KTINFDNGVN TTLGLPIIRT SPDHGTAFPL
310 320 330
AGKNKADEGA MVAALVMAAN SAHNRQAYAQ NSIDG
Length:335
Mass (Da):35,453
Last modified:February 1, 2005 - v1
Checksum:i399463F50DAB2773
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008692 Genomic DNA. Translation: AAV89937.1.
RefSeqiWP_011241114.1. NC_006526.2.
YP_163048.1. NC_006526.2.

Genome annotation databases

EnsemblBacteriaiAAV89937; AAV89937; ZMO1313.
KEGGizmo:ZMO1313.
PATRICi32567912. VBIZymMob102260_1244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008692 Genomic DNA. Translation: AAV89937.1.
RefSeqiWP_011241114.1. NC_006526.2.
YP_163048.1. NC_006526.2.

3D structure databases

ProteinModelPortaliQ5NMX3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264203.ZMO1313.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV89937; AAV89937; ZMO1313.
KEGGizmo:ZMO1313.
PATRICi32567912. VBIZymMob102260_1244.

Phylogenomic databases

eggNOGiCOG1995.
HOGENOMiHOG000221592.
KOiK00097.
OMAiDTLFQDK.
OrthoDBiEOG6GN6ZC.

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00557. pdxA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31821 / ZM4 / CP4.

Entry informationi

Entry nameiPDXA_ZYMMO
AccessioniPrimary (citable) accession number: Q5NMX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 1, 2005
Last modified: May 27, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is located at the dimer interface.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.