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Q5NMK1 (SYL_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:ZMO1435
OrganismZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) [Complete proteome] [HAMAP]
Taxonomic identifier264203 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas

Protein attributes

Sequence length846 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 846846Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152126

Regions

Motif47 – 5711"HIGH" region HAMAP-Rule MF_00049
Motif621 – 6255"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6241ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5NMK1 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: 3A727DDA60F0CB44

FASTA84695,428
        10         20         30         40         50         60 
MSESGFSQRF NPHVVDGRWQ KKWEESGCFH AKDNSDRPHS YILEMFPYPS GRIHMGHVRN 

        70         80         90        100        110        120 
YTMGDVLARY YRMKGHEVLH PMGWDAFGMP AENAAMERKI HPREWTMSNI ATMREQLKRI 

       130        140        150        160        170        180 
GFAIDWSREL ATCEPSYYGQ EQALFLDLYK AGLVYRKESA VNWDPIDNTV LANEQVIDGR 

       190        200        210        220        230        240 
GWRSGALVER KKLNQWFLKI TEFADDLLDG LKDLDQWPEK VRSMQENWIG RSQGMQFHFN 

       250        260        270        280        290        300 
FEVAPEGFDK IEVFTTRPDT LFGASFVAIA CDHPIAKALA EKNAALPEFI ADCQKMGTAA 

       310        320        330        340        350        360 
EDIETAEKKG FDTGLSLVHP LNPELKLPLF VANFVLMDYG TGAVFGCPAH DQRDLDFALK 

       370        380        390        400        410        420 
YNLPVKRVVA PSEAESNEAI GDKADTRAGI MVNSSFLDGL SSEEAKKTVI ARAEKEGWGK 

       430        440        450        460        470        480 
GTTVFRLRDW GVSRQRYWGT PIPIIHCDSC GAVPVPKDQL PVTLPDDINF DKPGNPLERH 

       490        500        510        520        530        540 
PTWKNVTCPK CGKPARRETD TLDTFVDSSW YFIRFASQPD DKPFDKATAE KWLPVGQYIG 

       550        560        570        580        590        600 
GVEHAILHLL YARFWTRALQ SIGRLDIKEP FTGLFTQGMV THETYKDPEG HWLSPEQIHK 

       610        620        630        640        650        660 
DEAGIFLTES GEKVTVGRVE KMSKSKKNVV EPAPILDQYG ADAVRWFMLS DSPPERDLAW 

       670        680        690        700        710        720 
TEAGIEGCWR FMQRLWRVAA IASEEASAGI DKDLQHRLHC SIKEVGEAIE GLSFNKAIAK 

       730        740        750        760        770        780 
IHDLVNAIEK AKASATRKEA ALTLFRLVAP MVPHLSEEAW HLLEKEGFVA EASWPEFDPA 

       790        800        810        820        830        840 
LTVEDEITIA VQVNGKLRDT LTVARDMPKD EAEKLALASE KVIKMLEGRS PKKVIVVPNR 


LVNIVA 

« Hide

References

[1]"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4."
Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. expand/collapse author list , Kang H.L., Lee S.Y., Lee K.J., Kang H.S.
Nat. Biotechnol. 23:63-68(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008692 Genomic DNA. Translation: AAV90059.1.
RefSeqYP_163170.1. NC_006526.2.

3D structure databases

ProteinModelPortalQ5NMK1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264203.ZMO1435.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV90059; AAV90059; ZMO1435.
GeneID3188964.
KEGGzmo:ZMO1435.
PATRIC32568142. VBIZymMob102260_1356.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ZYMMO
AccessionPrimary (citable) accession number: Q5NMK1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: February 1, 2005
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries