Q5NM38 (DXS2_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase 2
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase 2
Short name=DXP synthase 2
Short name=DXPS 2

Gene names

Name:	dxs2
Ordered Locus Names:	ZMO1598

Organism

Zymomonas mobilis [Complete proteome] [HAMAP]

Taxonomic identifier

542 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.
Sequence caution	The sequence AAV90222.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	1-deoxy-D-xylulose-5-phosphate synthase 2 HAMAP MF_00315		PRO_0000256511

Sequences

	Sequence		Length	Mass (Da)
	Q5NM38 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: B277FE4E395EEA24	FASTA	650	70,514
	10 20 30 40 50 60 MFPNKKTPLL DKIKTPAELR QLDRNSLRQL ADELRKETIS AVGVTGGHLG SGLGVIELTV 70 80 90 100 110 120 ALHYVFNTPK DALVWDVGHQ TYPHKILTGR RDRIRTLRQR DGLSGFTQRA ESEYDAFGAA 130 140 150 160 170 180 HSSTSISAAL GFAMASKLSD SDDKAVAIIG DGSMTAGMAY EAMNNAKAAG KRLIVILNDN 190 200 210 220 230 240 EMSISPPVGA LSSYLSRLIS SRPFMNLRDI MRGVVNRMPK GLATAARKAD EYARGMATGG 250 260 270 280 290 300 TFFEELGFYY VGPVDGHNLD QLIPVLENVR DAKDGPILVH VVTRKGQGYA PAEAAKDKYH 310 320 330 340 350 360 AVQRLDVVSG KQAKAPPGPP SYTSVFSEQL IKEAKQDDKI VTITAAMPTG TGLDRFQQYF 370 380 390 400 410 420 PERMFDVGIA EQHAVTFAAG LAAAGYKPFC CLYSTFLQRG YDQLVHDVAI QNLPVRFAVD 430 440 450 460 470 480 RAGLVGADGA THAGSFDLAF MVNLPNMVVM APSDERELAN MVHSMAHYDQ GPISVRYPRG 490 500 510 520 530 540 NGVGVSLEGE KEILPIGKGR LIRRGKKVAI LSLGTRLEES LKAADRLDAQ GLSTSVADMR 550 560 570 580 590 600 FAKPLDEALT RQLLKSHQVI ITIEEGALGG FATQVLTMAS DEGLMDDGLK IRTLRLPDRF 610 620 630 640 650 QPQDKQERQY AEAGLDADGI VAAVTAALQR NSKPVEVVEL TTKVTEDMTL

References

[1]

"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed: 15592456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008692 Genomic DNA. Translation: AAV90222.1. Different initiation.
RefSeq	YP_163333.1. NC_006526.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3187537.
GenomeReviews	Gene locus ZMO1598 in contig AE008692_GR.
KEGG	zmo:ZMO1598.
NMPDR	fig|264203.3.peg.72.
PATRIC	32568454. VBIZymMob102260_1503.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	EAMNAHA.
ProtClustDB	PRK12571.
Enzyme and pathway databases
BioCyc	ZMOB264203:ZMO1598-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS2_ZYMMO

Accession

Primary (citable) accession number: Q5NM38

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program