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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 42ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:ZMO1658
OrganismiZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
Taxonomic identifieri264203 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
Proteomesi
  • UP000001173 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001818131 – 329tRNA(Ile)-lysidine synthaseAdd BLAST329

Structurei

3D structure databases

ProteinModelPortaliQ5NLX8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000236508.
KOiK04075.
OMAiANTARYC.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5NLX8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGELRSLLL PELRLFNEAI KGILSSPLSG KLGIAVSGGS DSLALLLLGA
60 70 80 90 100
VSDFPVEAVT VDHGMRPEAA EEARFVAQIC QQIGVPHQIL TTKIEANGEG
110 120 130 140 150
MQAAARIRRY ALMAEWAKEK NVGALMTAHH ADDQAETFLM RAARGSGLNG
160 170 180 190 200
LAAIRPDVVI TAEGQKLRLL RPLLGFTRSA LAEIVEKAGF TFVSDPSNDN
210 220 230 240 250
PHYDRTHFRR LLKASPWINR QHIAEAARYC QQSEEAIEWI TAKEAAARIR
260 270 280 290 300
KKGEVLYLDP TELPIEILRR LVLRAMETIT GNTALRGSAL SRFIRSLQQG
310 320
KKVMLADVIG QGGEYWRFYR APPRKKAVL
Length:329
Mass (Da):36,295
Last modified:February 1, 2005 - v1
Checksum:iBED1B55404CAB03C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008692 Genomic DNA. Translation: AAV90282.1.
RefSeqiWP_011241407.1. NC_006526.2.

Genome annotation databases

EnsemblBacteriaiAAV90282; AAV90282; ZMO1658.
KEGGizmo:ZMO1658.
PATRICi32568580. VBIZymMob102260_1565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008692 Genomic DNA. Translation: AAV90282.1.
RefSeqiWP_011241407.1. NC_006526.2.

3D structure databases

ProteinModelPortaliQ5NLX8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV90282; AAV90282; ZMO1658.
KEGGizmo:ZMO1658.
PATRICi32568580. VBIZymMob102260_1565.

Phylogenomic databases

HOGENOMiHOG000236508.
KOiK04075.
OMAiANTARYC.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTILS_ZYMMO
AccessioniPrimary (citable) accession number: Q5NLX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: February 1, 2005
Last modified: September 7, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.