Reviewed, UniProtKB/Swiss-Prot Q5NL16 (PANB2_ZYMMO)

Names and origin

Protein names

Recommended name:
    3-methyl-2-oxobutanoate hydroxymethyltransferase 2
    EC=2.1.2.11
Alternative name(s):
    Ketopantoate hydroxymethyltransferase 2
      Short name=KPHMT 2

Gene names

Name:	panB2
Ordered Locus Names:	ZMO1970

Organism

Zymomonas mobilis [Complete proteome] [HAMAP]

Taxonomic identifier

542 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the panB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	3-methyl-2-oxobutanoate hydroxymethyltransferase 2 HAMAP MF_00156		PRO_0000297419
Regions
	Region	50 – 51	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	188	1	Proton acceptor By similarity
	Metal binding	50	1	Magnesium By similarity
	Metal binding	89	1	Magnesium By similarity
	Metal binding	121	1	Magnesium By similarity
	Binding site	89	1	Alpha-ketoisovalerate By similarity
	Binding site	119	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NL16-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 4F88F3A83CFD0AA9	FASTA	273	29,231
	10 20 30 40 50 60 MSAIPSSNKR RTIPELRARK GKSPIVALTA YSALTARFVD PYADFILVGD SLAMVEHGMA 70 80 90 100 110 120 TTIGASLDMM ILHGQSVMRG SEKAAVVIDM PFGSYEASPQ EAYHNAVRIL SETGCSAVKL 130 140 150 160 170 180 EGGSHLAPVI AFLTARGVPV MGHIGLTPQY VQTLGGFKIQ GHSSEQQDKI KQDALDFEAA 190 200 210 220 230 240 GAFSVVLEGV TEPLAREITD NIAIPTIGIG ASSYCDGQVL VLEDMLGFND KVPRFVKKFA 250 260 270 HLGDDIKKAV SDYATAVSNR SFPAEDNIYR PKS

References

[1]

"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed: 15592456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008692 Genomic DNA. Translation: AAV90594.1.
RefSeq	YP_163705.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3188177.
GenomeReviews	Gene locus ZMO1970 in contig AE008692_GR.
KEGG	zmo:ZMO1970.
NMPDR	fig|264203.3.peg.1382.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5NL16.
OMA	LVVVDMP.
Enzyme and pathway databases
BioCyc	ZMOB264203:ZMO1970-MON.
BRENDA	2.1.2.11. 1658.
Family and domain databases
HAMAP	MF_00156. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
TIGRFAMs	TIGR00222. panB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB2_ZYMMO

Accession

Primary (citable) accession number: Q5NL16

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	February 1, 2005
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)