Q5NIP6 (GATA_FRATT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A
Short name=Glu-ADT subunit A
EC=6.3.5.-

Gene names

Name:	gatA
Ordered Locus Names:	FTT_0020

Organism

Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) [Reference proteome] [HAMAP]

Taxonomic identifier

177416 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › Francisella tularensis › Francisella tularensis subsp. tularensis

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00120
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00120
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP-Rule MF_00120
Sequence similarities	Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP-Rule MF_00120		PRO_0000241103
Sites
	Active site	74	1	Charge relay system By similarity
	Active site	149	1	Charge relay system By similarity
	Active site	173	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NIP6 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: CA141F67B99F5F72	FASTA	481	52,303
	10 20 30 40 50 60 MSYIKKLRAR LDSGEISAVE LTKEYLAKIK EQDKRINSVI TLCEAEALKE AEDADAIISA 70 80 90 100 110 120 GKQGLLTGIP ILHKDLFCTK GIRTTAASKM LDNFVAPYDS TVTKNCKDQG MVTLGKLNMD 130 140 150 160 170 180 EFAMGSTNEY SYYGAVSNPW DLERVPGGSS GGSAAAVAAG FAPISTGSDT GGSVRQPASF 190 200 210 220 230 240 CGLTAMKPSY GSTSRFGMVA FASSFDQAGV LGHYAEDVAI MLDAIAGECE FDSTCVGVKQ 250 260 270 280 290 300 NHFTQDLEKD ISGKVIGVDE SLIKDLPAQI QEAVSKTLDN FKKLGAEIKS VKVPDLKEAL 310 320 330 340 350 360 STYYIITPAE AAANLARYDG IRYGYRNPEA RDLDELYRKS RTDGFGAEVK RRIMIGNYVL 370 380 390 400 410 420 ASSQYDSYYN KAQQLRKVMT DQINQIFTQV DAIFMPASPS EAFKKGDKLD PVSAYLSDIY 430 440 450 460 470 480 TIPANISGLP AIAFPIGFAN NLPVGGQLMA KAFNDNILTQ MVVQYQKHYG IEEFILQQAR I

References

[1]

"The complete genome sequence of Francisella tularensis, the causative agent of tularemia." Larsson P., Oyston P.C.F., Chain P., Chu M.C., Duffield M., Fuxelius H.-H., Garcia E., Haelltorp G., Johansson D., Isherwood K.E., Karp P.D., Larsson E., Liu Y., Michell S., Prior J., Prior R., Malfatti S., Sjoestedt A. , Svensson K., Thompson N., Vergez L., Wagg J.K., Wren B.W., Lindler L.E., Andersson S.G.E., Forsman M., Titball R.W. Nat. Genet. 37:153-159(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCHU S4 / Schu 4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ749949 Genomic DNA. Translation: CAG44653.1.
RefSeq	YP_169096.1. NC_006570.2.
3D structure databases
ProteinModelPortal	Q5NIP6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5NIP6. 15 interactions.
STRING	177416.FTT_0020.
Protocols and materials databases
DNASU	3191915.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG44653; CAG44653; FTT_0020.
GeneID	3191915.
KEGG	ftu:FTT_0020.
PATRIC	17962991. VBIFraTul2086_0023.
Phylogenomic databases
eggNOG	COG0154.
HOGENOM	HOG000116699.
KO	K02433.
OMA	RYDGVKY.
ProtClustDB	PRK00012.
Family and domain databases
Gene3D	3.90.1300.10. 1 hit.
HAMAP	MF_00120. GatA.
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view]
PANTHER	PTHR11895. PTHR11895. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
TIGRFAMs	TIGR00132. gatA. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATA_FRATT

Accession

Primary (citable) accession number: Q5NIP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program