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Q5NIP6 (GATA_FRATT) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A

Short name=Glu-ADT subunit A
EC=6.3.5.7
Gene names
Name:gatA
Ordered Locus Names:FTT_0020
OrganismFrancisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) [Reference proteome] [HAMAP]
Taxonomic identifier177416 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Protein attributes

Sequence length481 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00120

Catalytic activity

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00120

Subunit structure

Heterotrimer of A, B and C subunits By similarity. HAMAP-Rule MF_00120

Sequence similarities

Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 481481Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP-Rule MF_00120
PRO_0000241103

Sites

Active site741Charge relay system By similarity
Active site1491Charge relay system By similarity
Active site1731Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5NIP6 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: CA141F67B99F5F72

FASTA48152,303
        10         20         30         40         50         60 
MSYIKKLRAR LDSGEISAVE LTKEYLAKIK EQDKRINSVI TLCEAEALKE AEDADAIISA 

        70         80         90        100        110        120 
GKQGLLTGIP ILHKDLFCTK GIRTTAASKM LDNFVAPYDS TVTKNCKDQG MVTLGKLNMD 

       130        140        150        160        170        180 
EFAMGSTNEY SYYGAVSNPW DLERVPGGSS GGSAAAVAAG FAPISTGSDT GGSVRQPASF 

       190        200        210        220        230        240 
CGLTAMKPSY GSTSRFGMVA FASSFDQAGV LGHYAEDVAI MLDAIAGECE FDSTCVGVKQ 

       250        260        270        280        290        300 
NHFTQDLEKD ISGKVIGVDE SLIKDLPAQI QEAVSKTLDN FKKLGAEIKS VKVPDLKEAL 

       310        320        330        340        350        360 
STYYIITPAE AAANLARYDG IRYGYRNPEA RDLDELYRKS RTDGFGAEVK RRIMIGNYVL 

       370        380        390        400        410        420 
ASSQYDSYYN KAQQLRKVMT DQINQIFTQV DAIFMPASPS EAFKKGDKLD PVSAYLSDIY 

       430        440        450        460        470        480 
TIPANISGLP AIAFPIGFAN NLPVGGQLMA KAFNDNILTQ MVVQYQKHYG IEEFILQQAR 


I 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ749949 Genomic DNA. Translation: CAG44653.1.
RefSeqYP_169096.1. NC_006570.2.

3D structure databases

ProteinModelPortalQ5NIP6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ5NIP6. 15 interactions.
STRING177416.FTT_0020.

Protocols and materials databases

DNASU3191915.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAG44653; CAG44653; FTT_0020.
GeneID3191915.
KEGGftu:FTT_0020.
PATRIC17962991. VBIFraTul2086_0023.

Phylogenomic databases

eggNOGCOG0154.
HOGENOMHOG000116699.
KOK02433.
OMAFGYRCEN.
OrthoDBEOG61P6R9.
ProtClustDBPRK00012.

Enzyme and pathway databases

BioCycFTUL177416:GNBP-20-MONOMER.

Family and domain databases

Gene3D3.90.1300.10. 1 hit.
HAMAPMF_00120. GatA.
InterProIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERPTHR11895. PTHR11895. 1 hit.
PfamPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMSSF75304. SSF75304. 1 hit.
TIGRFAMsTIGR00132. gatA. 1 hit.
PROSITEPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGATA_FRATT
AccessionPrimary (citable) accession number: Q5NIP6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: February 1, 2005
Last modified: March 19, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families