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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei74 – 741Charge relay systemUniRule annotation
Active sitei149 – 1491Charge relay systemUniRule annotation
Active sitei173 – 1731Acyl-ester intermediateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciFTUL177416:GNBP-20-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:FTT_0020
OrganismiFrancisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)
Taxonomic identifieri177416 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
ProteomesiUP000001174 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481Glutamyl-tRNA(Gln) amidotransferase subunit APRO_0000241103Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

IntActiQ5NIP6. 15 interactions.
STRINGi177416.FTT_0020.

Structurei

3D structure databases

ProteinModelPortaliQ5NIP6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0154.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiEOG61P6R9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5NIP6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYIKKLRAR LDSGEISAVE LTKEYLAKIK EQDKRINSVI TLCEAEALKE
60 70 80 90 100
AEDADAIISA GKQGLLTGIP ILHKDLFCTK GIRTTAASKM LDNFVAPYDS
110 120 130 140 150
TVTKNCKDQG MVTLGKLNMD EFAMGSTNEY SYYGAVSNPW DLERVPGGSS
160 170 180 190 200
GGSAAAVAAG FAPISTGSDT GGSVRQPASF CGLTAMKPSY GSTSRFGMVA
210 220 230 240 250
FASSFDQAGV LGHYAEDVAI MLDAIAGECE FDSTCVGVKQ NHFTQDLEKD
260 270 280 290 300
ISGKVIGVDE SLIKDLPAQI QEAVSKTLDN FKKLGAEIKS VKVPDLKEAL
310 320 330 340 350
STYYIITPAE AAANLARYDG IRYGYRNPEA RDLDELYRKS RTDGFGAEVK
360 370 380 390 400
RRIMIGNYVL ASSQYDSYYN KAQQLRKVMT DQINQIFTQV DAIFMPASPS
410 420 430 440 450
EAFKKGDKLD PVSAYLSDIY TIPANISGLP AIAFPIGFAN NLPVGGQLMA
460 470 480
KAFNDNILTQ MVVQYQKHYG IEEFILQQAR I
Length:481
Mass (Da):52,303
Last modified:February 1, 2005 - v1
Checksum:iCA141F67B99F5F72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ749949 Genomic DNA. Translation: CAG44653.1.
RefSeqiWP_003022862.1. NZ_CP010290.1.
YP_169096.1. NC_006570.2.

Genome annotation databases

EnsemblBacteriaiCAG44653; CAG44653; FTT_0020.
GeneIDi3191915.
KEGGiftu:FTT_0020.
PATRICi17962991. VBIFraTul2086_0023.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ749949 Genomic DNA. Translation: CAG44653.1.
RefSeqiWP_003022862.1. NZ_CP010290.1.
YP_169096.1. NC_006570.2.

3D structure databases

ProteinModelPortaliQ5NIP6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5NIP6. 15 interactions.
STRINGi177416.FTT_0020.

Protocols and materials databases

DNASUi3191915.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG44653; CAG44653; FTT_0020.
GeneIDi3191915.
KEGGiftu:FTT_0020.
PATRICi17962991. VBIFraTul2086_0023.

Phylogenomic databases

eggNOGiCOG0154.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiEOG61P6R9.

Enzyme and pathway databases

BioCyciFTUL177416:GNBP-20-MONOMER.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SCHU S4 / Schu 4.

Entry informationi

Entry nameiGATA_FRATT
AccessioniPrimary (citable) accession number: Q5NIP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: February 1, 2005
Last modified: July 22, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.