Q5NIE5 (GLPK_FRATT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	FTT_0130

Organism

Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)

Taxonomic identifier

177416 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Glycerol kinase HAMAP MF_00186		PRO_1000058452
Regions
	Nucleotide binding	412 – 416	5	ATP By similarity
Sites
	Binding site	13	1	Substrate By similarity
	Binding site	17	1	ATP By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	268	1	ATP By similarity
	Binding site	311	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NIE5 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 5E0C65A57786AF58	FASTA	502	55,717
	10 20 30 40 50 60 MSKDFILAVD QGTTSSRAII FDKKGNIRKI AQKEFTQIYP KSGWVEHDAM EIWGTQSGVM 70 80 90 100 110 120 REALEFGRVK PDQIAAIGIT NQRETVVVWD KETGDPVYNA IVWQCRRTSS ICDEIKRDPQ 130 140 150 160 170 180 FVKYIKENTG LVVDAYFSGT KVKWILDNVE GAREKANAGK LLMGTIDTWL IWNLTRGKVH 190 200 210 220 230 240 ATDYSNASRT MLFNINSLEW DKKILDYLNI PESMLPEVKN SSEVFGVTDS HTLGGAEIPI 250 260 270 280 290 300 AGVAGDQHAA LFGHCCFEKG MAKNTYGTGC FALMNVGDKP VYSDEGLLTT IAWAENGKPT 310 320 330 340 350 360 YALEGSVFIA GAVIQWIRDG LGLVRSAEDS EYYATKIDST NGVYLVPAFV GLGTPYWDMY 370 380 390 400 410 420 ARGTIVGITR DTKREHIIRA ALEAIAYQAK DVLECMKEDT GLDLAGLRVD GGAVQNNFLM 430 440 450 460 470 480 QFQSDILQSE ISKPKVNEIT GLGAVFLAGL AVGFWKDKQE LKSILTTEKV FEPQKDSQAV 490 500 AHDYRGWKKA VERSKAWAEC YS

References

[1]

"The complete genome sequence of Francisella tularensis, the causative agent of tularemia." Larsson P., Oyston P.C.F., Chain P., Chu M.C., Duffield M., Fuxelius H.-H., Garcia E., Haelltorp G., Johansson D., Isherwood K.E., Karp P.D., Larsson E., Liu Y., Michell S., Prior J., Prior R., Malfatti S., Sjoestedt A. , Svensson K., Thompson N., Vergez L., Wagg J.K., Wren B.W., Lindler L.E., Andersson S.G.E., Forsman M., Titball R.W. Nat. Genet. 37:153-159(2005) [PubMed: 15640799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCHU S4 / Schu 4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ749949 Genomic DNA. Translation: CAG44763.1.
RefSeq	YP_169197.1. NC_006570.2.
3D structure databases
HSSP	HSSP built from PDB template 1GLC based on UniProtKB P0A6F3.
ProteinModelPortal	Q5NIE5.
SMR	Q5NIE5. Positions 3-499.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3192359.
GenomeReviews	Gene locus FTT_0130 in contig AJ749949_GR.
KEGG	ftu:FTT_0130.
PATRIC	17963240. VBIFraTul2086_0145.
Phylogenomic databases
HOGENOM	HBG511469.
OMA	ADTIGMP.
ProtClustDB	CLSK934381.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_FRATT

Accession

Primary (citable) accession number: Q5NIE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program