ID   DUT_FRATT               Reviewed;         148 AA.
AC   Q5NHX4;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-NOV-2009, entry version 27.
DE   RecName: Full=Deoxyuridine 5'-triphosphate nucleotidohydrolase;
DE            Short=dUTPase;
DE            EC=3.6.1.23;
DE   AltName: Full=dUTP pyrophosphatase;
GN   Name=dut; OrderedLocusNames=FTT0319;
OS   Francisella tularensis subsp. tularensis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
OC   Francisellaceae; Francisella.
OX   NCBI_TaxID=119856;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SCHU S4 / Schu 4;
RX   PubMed=15640799; DOI=10.1038/ng1499;
RA   Larsson P., Oyston P.C.F., Chain P., Chu M.C., Duffield M.,
RA   Fuxelius H.-H., Garcia E., Haelltorp G., Johansson D., Isherwood K.E.,
RA   Karp P.D., Larsson E., Liu Y., Michell S., Prior J., Prior R.,
RA   Malfatti S., Sjoestedt A., Svensson K., Thompson N., Vergez L.,
RA   Wagg J.K., Wren B.W., Lindler L.E., Andersson S.G.E., Forsman M.,
RA   Titball R.W.;
RT   "The complete genome sequence of Francisella tularensis, the causative
RT   agent of tularemia.";
RL   Nat. Genet. 37:153-159(2005).
CC   -!- FUNCTION: This enzyme is involved in nucleotide metabolism: it
CC       produces dUMP, the immediate precursor of thymidine nucleotides
CC       and it decreases the intracellular concentration of dUTP so that
CC       uracil cannot be incorporated into DNA (By similarity).
CC   -!- CATALYTIC ACTIVITY: dUTP + H(2)O = dUMP + diphosphate.
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- PATHWAY: Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP
CC       (dUTP route): step 2/2.
CC   -!- SIMILARITY: Belongs to the dUTPase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AJ749949; CAG44952.1; -; Genomic_DNA.
DR   RefSeq; YP_169368.1; -.
DR   GeneID; 3191551; -.
DR   GenomeReviews; AJ749949_GR; FTT0319.
DR   KEGG; ftu:FTT0319; -.
DR   HOGENOM; Q5NHX4; -.
DR   OMA; LDLRACI; -.
DR   BRENDA; 3.6.1.23; 297365.
DR   GO; GO:0004170; F:dUTP diphosphatase activity; IEA:HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR   GO; GO:0046080; P:dUTP metabolic process; IEA:InterPro.
DR   HAMAP; MF_00116; -; 1.
DR   InterPro; IPR008180; DeoxyUTP_pyroPase_dom.
DR   InterPro; IPR008181; dUTP_pyrophosphatase_subfam_1.
DR   Pfam; PF00692; dUTPase; 1.
DR   TIGRFAMs; TIGR00576; dut; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Hydrolase; Magnesium; Metal-binding;
KW   Nucleotide metabolism.
FT   CHAIN         1    148       Deoxyuridine 5'-triphosphate
FT                                nucleotidohydrolase.
FT                                /FTId=PRO_0000182862.
FT   REGION       67     69       Substrate binding (By similarity).
FT   REGION       84     86       Substrate binding (By similarity).
FT   BINDING      80     80       Substrate (By similarity).
FT   BINDING      94     94       Substrate; via amide nitrogen and
FT                                carbonyl oxygen (By similarity).
SQ   SEQUENCE   148 AA;  15942 MW;  A8BB5F2950FBB081 CRC64;
     MKVELKILNK ELIKELPGYA TEGSAAIDLR ACISESIYLK SGECKLVATG IAINIANPNY
     AAMILPRSGL GHKKGLVLGN GTGLIDSDYQ GELMVSCFNR SQETIEIEPL MRFAQLVIVP
     VVQANFEIVE DFSQQSVRAT GGFGHTGV
//