Reviewed, UniProtKB/Swiss-Prot Q5NHX4 (DUT_FRATT)

Names and origin

Protein names

Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolase
      Short name=dUTPase
    EC=3.6.1.23
Alternative name(s):
    dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	FTT0319

Organism

Francisella tularensis subsp. tularensis [Complete proteome] [HAMAP]

Taxonomic identifier

119856 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity.
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_0000182862
Regions
	Region	67 – 69	3	Substrate binding By similarity
	Region	84 – 86	3	Substrate binding By similarity
Sites
	Binding site	80	1	Substrate By similarity
	Binding site	94	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NHX4-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: A8BB5F2950FBB081	FASTA	148	15,942
	10 20 30 40 50 60 MKVELKILNK ELIKELPGYA TEGSAAIDLR ACISESIYLK SGECKLVATG IAINIANPNY 70 80 90 100 110 120 AAMILPRSGL GHKKGLVLGN GTGLIDSDYQ GELMVSCFNR SQETIEIEPL MRFAQLVIVP 130 140 VVQANFEIVE DFSQQSVRAT GGFGHTGV

References

[1]

"The complete genome sequence of Francisella tularensis, the causative agent of tularemia." Larsson P., Oyston P.C.F., Chain P., Chu M.C., Duffield M., Fuxelius H.-H., Garcia E., Haelltorp G., Johansson D., Isherwood K.E., Karp P.D., Larsson E., Liu Y., Michell S., Prior J., Prior R., Malfatti S., Sjoestedt A. , Svensson K., Thompson N., Vergez L., Wagg J.K., Wren B.W., Lindler L.E., Andersson S.G.E., Forsman M., Titball R.W. Nat. Genet. 37:153-159(2005) [PubMed: 15640799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCHU S4 / Schu 4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ749949 Genomic DNA. Translation: CAG44952.1.
RefSeq	YP_169368.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3191551.
GenomeReviews	Gene locus FTT0319 in contig AJ749949_GR.
KEGG	ftu:FTT0319.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5NHX4.
OMA	LDLRACI.
Enzyme and pathway databases
BRENDA	3.6.1.23. 297365.
Family and domain databases
HAMAP	MF_00116. [Tree]
InterPro	IPR008180. DeoxyUTP_pyroPase_dom. IPR008181. dUTP_pyrophosphatase_subfam_1. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_FRATT

Accession

Primary (citable) accession number: Q5NHX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	February 1, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)