Q5NH50 (KITH_FRATT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	FTT_0621

Organism

Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) [Reference proteome] [HAMAP]

Taxonomic identifier

177416 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › Francisella tularensis › Francisella tularensis subsp. tularensis

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	Thymidine kinase HAMAP-Rule MF_00124		PRO_0000174976
Regions
	Nucleotide binding	9 – 16	8	ATP By similarity
	Nucleotide binding	87 – 90	4	ATP By similarity
Sites
	Active site	88	1	Proton acceptor Potential
	Metal binding	145	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity
	Metal binding	187	1	Zinc By similarity
	Metal binding	190	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NH50 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 5D38405A57377313	FASTA	197	22,473
	10 20 30 40 50 60 MAKLYFRYSA MDAGKTLDLL KVAYNYEDRG RKPLVLTSAI DKRAGLNKVK SRIGINQDAY 70 80 90 100 110 120 SLTDRDNIFE FVENYNSSNK IDCVLIDEIH FFTQEQVWQL AEIVDELNIP VICYGLRTNY 130 140 150 160 170 180 LGQPFETAAL LLAIADTLEE VKTICHCGKK ASFNMMVQNG KAIKQGNPIV VDDDSLKEID 190 TKYVSVCRKH WKEGVYE

References

[1]

"The complete genome sequence of Francisella tularensis, the causative agent of tularemia." Larsson P., Oyston P.C.F., Chain P., Chu M.C., Duffield M., Fuxelius H.-H., Garcia E., Haelltorp G., Johansson D., Isherwood K.E., Karp P.D., Larsson E., Liu Y., Michell S., Prior J., Prior R., Malfatti S., Sjoestedt A. , Svensson K., Thompson N., Vergez L., Wagg J.K., Wren B.W., Lindler L.E., Andersson S.G.E., Forsman M., Titball R.W. Nat. Genet. 37:153-159(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCHU S4 / Schu 4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ749949 Genomic DNA. Translation: CAG45254.1.
RefSeq	YP_169642.1. NC_006570.2.
3D structure databases
ProteinModelPortal	Q5NH50.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5NH50. 1 interaction.
STRING	177416.FTT_0621.
Protocols and materials databases
DNASU	3190754.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG45254; CAG45254; FTT_0621.
GeneID	3190754.
KEGG	ftu:FTT_0621.
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076391.
KO	K00857.
OMA	FRYGAMN.
ProtClustDB	PRK04296.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_FRATT

Accession

Primary (citable) accession number: Q5NH50

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program