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Protein

Bifunctional protein FolD

Gene

folD

Organism
Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei231NADP; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 167NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:FTT_0892
OrganismiFrancisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)
Taxonomic identifieri177416 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
Proteomesi
  • UP000001174 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002683521 – 282Bifunctional protein FolDAdd BLAST282

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiQ5NGF3. 1 interactor.
STRINGi177416.FTT_0892.

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 28Combined sources23
Beta strandi33 – 40Combined sources8
Helixi43 – 58Combined sources16
Beta strandi62 – 68Combined sources7
Helixi74 – 85Combined sources12
Beta strandi92 – 95Combined sources4
Helixi105 – 111Combined sources7
Helixi114 – 116Combined sources3
Helixi123 – 131Combined sources9
Helixi140 – 151Combined sources12
Beta strandi160 – 164Combined sources5
Turni168 – 170Combined sources3
Helixi171 – 180Combined sources10
Beta strandi184 – 188Combined sources5
Helixi195 – 199Combined sources5
Beta strandi203 – 207Combined sources5
Helixi217 – 219Combined sources3
Beta strandi225 – 228Combined sources4
Beta strandi232 – 234Combined sources3
Beta strandi237 – 239Combined sources3
Helixi244 – 247Combined sources4
Turni248 – 250Combined sources3
Beta strandi252 – 254Combined sources3
Beta strandi257 – 261Combined sources5
Helixi264 – 279Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3L07X-ray1.88A/B1-282[»]
ProteinModelPortaliQ5NGF3.
SMRiQ5NGF3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5NGF3.

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CN0. Bacteria.
COG0190. LUCA.
HOGENOMiHOG000218242.
KOiK01491.
OMAiLMFNTIK.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5NGF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILIDGKSLS KDLKERLATQ VQEYKHHTAI TPKLVAIIVG NDPASKTYVA
60 70 80 90 100
SKEKACAQVG IDSQVITLPE HTTESELLEL IDQLNNDSSV HAILVQLPLP
110 120 130 140 150
AHINKNNVIY SIKPEKDVDG FHPTNVGRLQ LRDKKCLESC TPKGIMTMLR
160 170 180 190 200
EYGIKTEGAY AVVVGASNVV GKPVSQLLLN AKATVTTCHR FTTDLKSHTT
210 220 230 240 250
KADILIVAVG KPNFITADMV KEGAVVIDVG INHVDGKIVG DVDFAAVKDK
260 270 280
VAAITPVPGG VGPMTITELL YNTFQCAQEL NR
Length:282
Mass (Da):30,454
Last modified:February 1, 2005 - v1
Checksum:i0DD0F5FEF81CEF09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ749949 Genomic DNA. Translation: CAG45525.1.
RefSeqiWP_003020886.1. NZ_CP010290.1.
YP_169889.1. NC_006570.2.

Genome annotation databases

EnsemblBacteriaiCAG45525; CAG45525; FTT_0892.
GeneIDi3191934.
KEGGiftu:FTT_0892.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ749949 Genomic DNA. Translation: CAG45525.1.
RefSeqiWP_003020886.1. NZ_CP010290.1.
YP_169889.1. NC_006570.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3L07X-ray1.88A/B1-282[»]
ProteinModelPortaliQ5NGF3.
SMRiQ5NGF3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5NGF3. 1 interactor.
STRINGi177416.FTT_0892.

Protocols and materials databases

DNASUi3191934.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG45525; CAG45525; FTT_0892.
GeneIDi3191934.
KEGGiftu:FTT_0892.

Phylogenomic databases

eggNOGiENOG4105CN0. Bacteria.
COG0190. LUCA.
HOGENOMiHOG000218242.
KOiK01491.
OMAiLMFNTIK.

Enzyme and pathway databases

UniPathwayiUPA00193.

Miscellaneous databases

EvolutionaryTraceiQ5NGF3.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOLD_FRATT
AccessioniPrimary (citable) accession number: Q5NGF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: February 1, 2005
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.