Q5NFK5 (TYSY_FRATT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	FTT_1229

Organism

Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) [Reference proteome] [HAMAP]

Taxonomic identifier

177416 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › Francisella tularensis › Francisella tularensis subsp. tularensis

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Thymidylate synthase HAMAP-Rule MF_00008		PRO_0000140959
Sites
	Active site	156	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NFK5 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: A1AFE1C25D79A803	FASTA	274	31,325
	10 20 30 40 50 60 MQEYLNFLKY IKENGVLKGD RTGTGTRSIF GYQMRFDLQK GFPLVTTKKI HIPSVVHELL 70 80 90 100 110 120 WFLSGSTNIK YLNDNNVRIW NEWATVDGEL GPIYGKQWRD FNGQGIDQIA DVIQMLKTNP 130 140 150 160 170 180 NSRRILVSAW NPCVVPSEKI SPQENVVKGN SALPPCHAMF QFYVANNKLS CMLTQRSADA 190 200 210 220 230 240 FLGVPFNIAS YSLLTNMVAQ QCNLDVGEFI WSGGDCHIYN NHIEQVNEQL SREPLALPTL 250 260 270 KILRKPNSIF DYKYEDFEFE NYNHHPAIKA KISV

References

[1]

"The complete genome sequence of Francisella tularensis, the causative agent of tularemia." Larsson P., Oyston P.C.F., Chain P., Chu M.C., Duffield M., Fuxelius H.-H., Garcia E., Haelltorp G., Johansson D., Isherwood K.E., Karp P.D., Larsson E., Liu Y., Michell S., Prior J., Prior R., Malfatti S., Sjoestedt A. , Svensson K., Thompson N., Vergez L., Wagg J.K., Wren B.W., Lindler L.E., Andersson S.G.E., Forsman M., Titball R.W. Nat. Genet. 37:153-159(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCHU S4 / Schu 4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ749949 Genomic DNA. Translation: CAG45862.1.
RefSeq	YP_170187.1. NC_006570.2.
3D structure databases
ProteinModelPortal	Q5NFK5.
SMR	Q5NFK5. Positions 2-274.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5NFK5. 1 interaction.
STRING	177416.FTT_1229.
Protocols and materials databases
DNASU	3190786.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG45862; CAG45862; FTT_1229.
GeneID	3190786.
KEGG	ftu:FTT_1229.
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HOG000257899.
KO	K00560.
OMA	DTNVAYL.
ProtClustDB	PRK01827.
Enzyme and pathway databases
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 1 hit.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_FRATT

Accession

Primary (citable) accession number: Q5NFK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program