Reviewed, UniProtKB/Swiss-Prot Q5NEH3 (TPMT_FRATT)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	FTT1661

Organism

Francisella tularensis subsp. tularensis [Complete proteome] [HAMAP]

Taxonomic identifier

119856 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_0000220119
Sites
	Binding site	16	1	S-adenosyl-L-methionine By similarity
	Binding site	51	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	72	1	S-adenosyl-L-methionine By similarity
	Binding site	131	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NEH3-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 640FFDD07F5E920C	FASTA	226	26,386
	10 20 30 40 50 60 MNKLETNNNQ YWLDRWQNDD VGFCQESPNE FLVKHFSKLN INDSSVCLIP MCGCSIDMLF 70 80 90 100 110 120 FLSKGVKVIG IELSEKAVLS FFSQNTINYE VIHGNDYKLY KGDDIEIYVA DIFNLPKIAN 130 140 150 160 170 180 NLPVFDIWYD RGAYIALPND LRTNYAKMML EVCSNNTQIL LLVMEHDKKS QTPPYSVTQA 190 200 210 220 ELIKNFSAKI KFELIDSKQR DNIPDYRKAE GMTEQYYTTY LRKKQY

References

[1]

"The complete genome sequence of Francisella tularensis, the causative agent of tularemia." Larsson P., Oyston P.C.F., Chain P., Chu M.C., Duffield M., Fuxelius H.-H., Garcia E., Haelltorp G., Johansson D., Isherwood K.E., Karp P.D., Larsson E., Liu Y., Michell S., Prior J., Prior R., Malfatti S., Sjoestedt A. , Svensson K., Thompson N., Vergez L., Wagg J.K., Wren B.W., Lindler L.E., Andersson S.G.E., Forsman M., Titball R.W. Nat. Genet. 37:153-159(2005) [PubMed: 15640799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCHU S4 / Schu 4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ749949 Genomic DNA. Translation: CAG46294.1.
RefSeq	YP_170569.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3190790.
GenomeReviews	Gene locus FTT1661 in contig AJ749949_GR.
KEGG	ftu:FTT1661.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5NEH3.
OMA	PPFAVSP.
Enzyme and pathway databases
BRENDA	2.1.1.67. 297365.
Family and domain databases
HAMAP	MF_00812. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_FRATT

Accession

Primary (citable) accession number: Q5NEH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	February 1, 2005
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)