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Protein

EGF domain-specific O-linked N-acetylglucosamine transferase

Gene

EOGT

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine.
UDP-N-acetyl-D-glucosamine + [protein]-L-threonine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-threonine.

GO - Molecular functioni

  1. protein N-acetylglucosaminyltransferase activity Source: UniProtKB

GO - Biological processi

  1. protein O-linked glycosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Protein family/group databases

CAZyiGT61. Glycosyltransferase Family 61.

Names & Taxonomyi

Protein namesi
Recommended name:
EGF domain-specific O-linked N-acetylglucosamine transferase (EC:2.4.1.255)
Alternative name(s):
Extracellular O-linked N-acetylglucosamine transferase
Gene namesi
Name:EOGT
Synonyms:AER61
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Unplaced

Subcellular locationi

Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 527510EGF domain-specific O-linked N-acetylglucosamine transferasePRO_0000301971Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi354 – 3541N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000009819.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi295 – 2973Required for optimal activityBy similarity
Motifi524 – 5274Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase 61 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG320328.
HOGENOMiHOG000033829.
HOVERGENiHBG056678.
InParanoidiQ5NDL9.
KOiK18134.
OrthoDBiEOG7D2FDQ.

Family and domain databases

InterProiIPR007657. Glycosyltransferase_AER61.
[Graphical view]
PfamiPF04577. DUF563. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5NDL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKLLVLGVL LHDVSLSGQD EAPPKADGIP GEPLFNYASI RLPEEHIPFF
60 70 80 90 100
LHNNRHIATV CKKDSHCPYK KHLENLKYCW GYEKSCKPEF RFGYPVCTYI
110 120 130 140 150
DMGWTDTLES AQDIFWKQAD FGYAGERLEE LHVLCQPEEP HDSSLLCSRY
160 170 180 190 200
LQYCRAANLY LDLRNIKRNH DRFKEDFFQS GEIGGHCTLD TQTLLSEGQR
210 220 230 240 250
KSPLQSWFAE LQSYTELNFR PIEDAKCDVV IEKPTYFMKL DAGVNMYHHF
260 270 280 290 300
CDFVNLYITQ HVNNSFSTDV YIVMWDTSSY GYGDLFSDTW KAFTDYDVIH
310 320 330 340 350
LKTYDSKRVC FKEAVFSLLP RMRYGLFYNT PLISGCQNTG LFRAFSQHVL
360 370 380 390 400
HRLNITQEGP KDGKIRVTIL ARSTEYRKIL NQNELVNALK TVSTLEVQIV
410 420 430 440 450
DYKYKELGFL DQLRITHNTD IFIGMHGAGL THLLFLPDWA AVFELYNCED
460 470 480 490 500
ERCYLDLARL RGVHYITWRR QNKVFPQDKG HHPTLGEHPK FTNYSFDVEE
510 520
FMYLVLQAAD HVLQHPKWPF KKKRDEL
Length:527
Mass (Da):61,670
Last modified:February 1, 2005 - v1
Checksum:iDA5DE507862BB6AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ868227 mRNA. Translation: CAI30562.1.
RefSeqiNP_001009187.1. NM_001009187.1.
XP_005632096.1. XM_005632039.1.
XP_005632097.1. XM_005632040.1.
XP_005632098.1. XM_005632041.1.
UniGeneiCfa.16220.

Genome annotation databases

GeneIDi494221.
KEGGicfa:494221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ868227 mRNA. Translation: CAI30562.1.
RefSeqiNP_001009187.1. NM_001009187.1.
XP_005632096.1. XM_005632039.1.
XP_005632097.1. XM_005632040.1.
XP_005632098.1. XM_005632041.1.
UniGeneiCfa.16220.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000009819.

Protein family/group databases

CAZyiGT61. Glycosyltransferase Family 61.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi494221.
KEGGicfa:494221.

Organism-specific databases

CTDi285203.

Phylogenomic databases

eggNOGiNOG320328.
HOGENOMiHOG000033829.
HOVERGENiHBG056678.
InParanoidiQ5NDL9.
KOiK18134.
OrthoDBiEOG7D2FDQ.

Miscellaneous databases

NextBioi20865674.

Family and domain databases

InterProiIPR007657. Glycosyltransferase_AER61.
[Graphical view]
PfamiPF04577. DUF563. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Phylogeny of xylosyltransferases."
    Kiefer-Meyer M.C., Pagny S., Durambure G., Faye L., Gomord V., Mollicone R., Oriol R.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiEOGT_CANFA
AccessioniPrimary (citable) accession number: Q5NDL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: February 1, 2005
Last modified: January 7, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.