Q5NDL9 (EOGT_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
EGF domain-specific O-linked N-acetylglucosamine transferase
EC=2.4.1.255

Alternative name(s):
Extracellular O-linked N-acetylglucosamine transferase

Gene names

Name:	EOGT
Synonyms:	AER61

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine. UDP-N-acetyl-D-glucosamine + [protein]-L-threonine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-threonine.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the glycosyltransferase 61 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein O-linked glycosylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein N-acetylglucosaminyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 527	510	EGF domain-specific O-linked N-acetylglucosamine transferase		PRO_0000301971
Regions
	Motif	524 – 527	4	Prevents secretion from ER Potential
Amino acid modifications
	Glycosylation	354	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5NDL9 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: DA5DE507862BB6AA	FASTA	527	61,670
	10 20 30 40 50 60 MLKLLVLGVL LHDVSLSGQD EAPPKADGIP GEPLFNYASI RLPEEHIPFF LHNNRHIATV 70 80 90 100 110 120 CKKDSHCPYK KHLENLKYCW GYEKSCKPEF RFGYPVCTYI DMGWTDTLES AQDIFWKQAD 130 140 150 160 170 180 FGYAGERLEE LHVLCQPEEP HDSSLLCSRY LQYCRAANLY LDLRNIKRNH DRFKEDFFQS 190 200 210 220 230 240 GEIGGHCTLD TQTLLSEGQR KSPLQSWFAE LQSYTELNFR PIEDAKCDVV IEKPTYFMKL 250 260 270 280 290 300 DAGVNMYHHF CDFVNLYITQ HVNNSFSTDV YIVMWDTSSY GYGDLFSDTW KAFTDYDVIH 310 320 330 340 350 360 LKTYDSKRVC FKEAVFSLLP RMRYGLFYNT PLISGCQNTG LFRAFSQHVL HRLNITQEGP 370 380 390 400 410 420 KDGKIRVTIL ARSTEYRKIL NQNELVNALK TVSTLEVQIV DYKYKELGFL DQLRITHNTD 430 440 450 460 470 480 IFIGMHGAGL THLLFLPDWA AVFELYNCED ERCYLDLARL RGVHYITWRR QNKVFPQDKG 490 500 510 520 HHPTLGEHPK FTNYSFDVEE FMYLVLQAAD HVLQHPKWPF KKKRDEL

References

[1]	"Phylogeny of xylosyltransferases." Kiefer-Meyer M.C., Pagny S., Durambure G., Faye L., Gomord V., Mollicone R., Oriol R. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ868227 mRNA. Translation: CAI30562.1.
RefSeq	NP_001009187.1. NM_001009187.1.
UniGene	Cfa.16220.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	9615.ENSCAFP00000009819.
Protein family/group databases
CAZy	GT61. Glycosyltransferase Family 61.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	494221.
KEGG	cfa:494221.
Organism-specific databases
CTD	285203.
Phylogenomic databases
eggNOG	NOG320328.
HOGENOM	HOG000033829.
HOVERGEN	HBG056678.
InParanoid	Q5NDL9.
OrthoDB	EOG4XGZZQ.
Family and domain databases
InterPro	IPR007657. Glycosyltransferase_AER61. [Graphical view]
Pfam	PF04577. DUF563. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20865674.

Entry information

Entry name

EOGT_CANFA

Accession

Primary (citable) accession number: Q5NDL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	February 1, 2005
Last modified:	April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program