Q5NDL2 (EOGT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
EGF domain-specific O-linked N-acetylglucosamine transferase
EC=2.4.1.255

Alternative name(s):
Extracellular O-linked N-acetylglucosamine transferase

Gene names

Name:	EOGT
Synonyms:	AER61, C3orf64, EOGT1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine. UDP-N-acetyl-D-glucosamine + [protein]-L-threonine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-threonine.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the glycosyltransferase 61 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein O-linked glycosylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein N-acetylglucosaminyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5NDL2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5NDL2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     208-208: F → C
     209-527: Missing.

Isoform 3 (identifier: Q5NDL2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     278-361: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 527	510	EGF domain-specific O-linked N-acetylglucosamine transferase		PRO_0000301970
Regions
	Motif	524 – 527	4	Prevents secretion from ER Potential
Amino acid modifications
	Glycosylation	354	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	208	1	F → C in isoform 2.		VSP_027897
	Alternative sequence	209 – 527	319	Missing in isoform 2.		VSP_027898
	Alternative sequence	278 – 361	84	Missing in isoform 3.		VSP_027899
Experimental info
	Sequence conflict	199	1	Q → R in BAC86479. Ref.2
	Sequence conflict	247	1	Y → C in CAE45897. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: C34D267187BFDDA9	FASTA	527	62,011
	10 20 30 40 50 60 MLMLFVFGVL LHEVSLSGQN EAPPNTHSIP GEPLYNYASI RLPEEHIPFF LHNNRHIATV 70 80 90 100 110 120 CRKDSLCPYK KHLEKLKYCW GYEKSCKPEF RFGYPVCSYV DMGWTDTLES AEDIFWKQAD 130 140 150 160 170 180 FGYARERLEE MHVLCQPKET SDSSLVCSRY LQYCRATNLY LDLRNIKRNH DRFKEDFFQS 190 200 210 220 230 240 GEIGGHCKLD IRTLTSEGQR KSPLQSWFAE LQSYTQLNFR PIEDAKCDIV IEKPTYFMKL 250 260 270 280 290 300 DAGVNMYHHF CDFINLYITQ HVNNSFSTDV YIVMWDTSSY GYGDLFSDTW NAFTDYDVIH 310 320 330 340 350 360 LKTYDSKRVC FKEAVFSLLP RMRYGLFYNT PLISGCQNTG LFRAFAQHVL HRLNITQEGP 370 380 390 400 410 420 KDGKIRVTIL ARSTEYRKIL NQNELVNALK TVSTFEVQIV DYKYRELGFL DQLRITHNTD 430 440 450 460 470 480 IFIGMHGAGL THLLFLPDWA AVFELYNCED ERCYLDLARL RGVHYITWRR QNKVFPQDKG 490 500 510 520 HHPTLGEHPK FTNYSFDVEE FMYLVLQAAD HVLQHPKWPF KKKHDEL
	Isoform 2 [UniParc]. Checksum: 387DDF61D5A96C3A	FASTA	208	24,403
	10 20 30 40 50 60 MLMLFVFGVL LHEVSLSGQN EAPPNTHSIP GEPLYNYASI RLPEEHIPFF LHNNRHIATV 70 80 90 100 110 120 CRKDSLCPYK KHLEKLKYCW GYEKSCKPEF RFGYPVCSYV DMGWTDTLES AEDIFWKQAD 130 140 150 160 170 180 FGYARERLEE MHVLCQPKET SDSSLVCSRY LQYCRATNLY LDLRNIKRNH DRFKEDFFQS 190 200 GEIGGHCKLD IRTLTSEGQR KSPLQSWC
	Isoform 3 [UniParc]. Checksum: EB338AE6E854889F	FASTA	443	52,297
	10 20 30 40 50 60 MLMLFVFGVL LHEVSLSGQN EAPPNTHSIP GEPLYNYASI RLPEEHIPFF LHNNRHIATV 70 80 90 100 110 120 CRKDSLCPYK KHLEKLKYCW GYEKSCKPEF RFGYPVCSYV DMGWTDTLES AEDIFWKQAD 130 140 150 160 170 180 FGYARERLEE MHVLCQPKET SDSSLVCSRY LQYCRATNLY LDLRNIKRNH DRFKEDFFQS 190 200 210 220 230 240 GEIGGHCKLD IRTLTSEGQR KSPLQSWFAE LQSYTQLNFR PIEDAKCDIV IEKPTYFMKL 250 260 270 280 290 300 DAGVNMYHHF CDFINLYITQ HVNNSFSTDV YIVMWDTDGK IRVTILARST EYRKILNQNE 310 320 330 340 350 360 LVNALKTVST FEVQIVDYKY RELGFLDQLR ITHNTDIFIG MHGAGLTHLL FLPDWAAVFE 370 380 390 400 410 420 LYNCEDERCY LDLARLRGVH YITWRRQNKV FPQDKGHHPT LGEHPKFTNY SFDVEEFMYL 430 440 VLQAADHVLQ HPKWPFKKKH DEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phylogeny of xylosyltransferases." Kiefer-Meyer M.C., Pagny S., Durambure G., Faye L., Gomord V., Mollicone R., Oriol R. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Placenta.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 69-527 (ISOFORM 1). Tissue: Esophageal carcinoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ868234 mRNA. Translation: CAI30569.1. AK126187 mRNA. Translation: BAC86479.1. BC060887 mRNA. Translation: AAH60887.1. BX640821 mRNA. Translation: CAE45897.2.
IPI	IPI00396231. IPI00747743. IPI00856087.
RefSeq	NP_775925.1. NM_173654.1.
UniGene	Hs.518059.
3D structure databases
ProteinModelPortal	Q5NDL2.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000295571.
Protein family/group databases
CAZy	GT61. Glycosyltransferase Family 61.
PTM databases
PhosphoSite	Q5NDL2.
Polymorphism databases
DMDM	74708096.
Proteomic databases
PaxDb	Q5NDL2.
PRIDE	Q5NDL2.
Protocols and materials databases
DNASU	285203.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000295571; ENSP00000295571; ENSG00000163378. ENST00000383701; ENSP00000373206; ENSG00000163378.
GeneID	285203.
KEGG	hsa:285203.
UCSC	uc003dnk.3. human. uc003dnl.3. human.
Organism-specific databases
CTD	285203.
GeneCards	GC03M069025.
HGNC	HGNC:28526. EOGT.
HPA	HPA019460.
MIM	614789. gene.
neXtProt	NX_Q5NDL2.
PharmGKB	PA143485338.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG320328.
HOGENOM	HOG000033829.
HOVERGEN	HBG056678.
InParanoid	Q5NDL2.
OMA	DSKRVCF.
OrthoDB	EOG4XGZZQ.
Gene expression databases
ArrayExpress	Q5NDL2.
Bgee	Q5NDL2.
CleanEx	HS_C3orf64.
Genevestigator	Q5NDL2.
Family and domain databases
InterPro	IPR007657. Glycosyltransferase_AER61. [Graphical view]
Pfam	PF04577. DUF563. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	285203.
NextBio	95347.
SOURCE	Search...

Entry information

Entry name

EOGT_HUMAN

Accession

Primary (citable) accession number: Q5NDL2
Secondary accession number(s): Q6MZY0, Q6P985, Q6ZTV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	February 1, 2005
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.