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Protein

Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2

Gene

POMGNT2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

O-linked mannose beta-1,4-N-acetylglucosaminyltransferase that tranfers UDP-N-acetyl-D-glucosamine to the 4-position of the mannose to generate N-acetyl-D-glucosamine-beta-1,4-O-D-mannosylprotein. Involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + O-alpha-D-mannosylprotein = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-O-alpha-D-mannosylprotein.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT61. Glycosyltransferase Family 61.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 (EC:2.4.1.-By similarity)
Short name:
POMGnT2
Alternative name(s):
Extracellular O-linked N-acetylglucosamine transferase-like
Glycosyltransferase-like domain-containing protein 2
Gene namesi
Name:POMGNT2
Synonyms:AGO61, GTDC2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 22

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44CytoplasmicSequence analysis
Transmembranei5 – 2521Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini26 – 580555LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2PRO_0000249012Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence analysis
Glycosylationi276 – 2761N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5NDF2.
PRIDEiQ5NDF2.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000583.

Structurei

3D structure databases

ProteinModelPortaliQ5NDF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini488 – 58093Fibronectin type-IIIPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 61 family.Curated
Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4698. Eukaryota.
ENOG410ZHQA. LUCA.
GeneTreeiENSGT00770000120599.
HOGENOMiHOG000006878.
HOVERGENiHBG054852.
InParanoidiQ5NDF2.
KOiK18207.
OMAiYAVNPDH.
OrthoDBiEOG72G16T.
TreeFamiTF332712.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR007657. Glycosyltransferase_AER61.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF04577. DUF563. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5NDF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLSAVLNAL LVSVLAAVLW KHVRLREHAA SLEEELAVGR RAADPAPALR
60 70 80 90 100
IDYPKALQIL TEGGTHMVCT GRTHTDRLCR FKWLCYSSEA EEFIFFHGNA
110 120 130 140 150
SVMLPSLGSR RFQPALLDLS TVEDHNTQYF NFVELPAAAL RFMPKPVFVP
160 170 180 190 200
DVALIANRFN PDNLMHVFHD DLLPLFYTLR QFPGLAREAR LFFMEGWGEG
210 220 230 240 250
AHFDLYKLLS PKQPLLRAQL KALGRLLCFS HAFVGLSKVT TWYQYGFVQP
260 270 280 290 300
QGPKANILVS GNEIRQFAHF LMEKLNVSQA GGPLGEEYIL VFSRTQNRLI
310 320 330 340 350
LNEAELLLAL AQEFQMKTVT VSLEDHAFAD VVRLVSNASM LVSMHGAQLV
360 370 380 390 400
TALFLPRGAA VVELFPYAVN PDHYTPYKTL ATLPGMDLQY IAWQNTMPEN
410 420 430 440 450
TVTHPERPWD QGGIAHLDRA EQARILQSRE VPRHLCCRNP EWLFRIYQDT
460 470 480 490 500
KVDIPSLIQT IRRVVKGHPG PRKQKWTVSL YPGKVREARC QASVQGASEA
510 520 530 540 550
RLSVSWQIPW NLKYLKVREV KYEVWLQEQG ENTYVPYMLA LQNHTFTENI
560 570 580
KPFTTYLVWI RCIFNKTLLG PFADVLVCST
Length:580
Mass (Da):66,067
Last modified:February 1, 2005 - v1
Checksum:iDA2AD2E3140D6D6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ868532 mRNA. Translation: CAI30866.1.
BC123822 mRNA. Translation: AAI23823.1.
RefSeqiNP_001011682.1. NM_001011682.2.
XP_005222438.1. XM_005222381.3.
UniGeneiBt.13504.

Genome annotation databases

EnsembliENSBTAT00000000583; ENSBTAP00000000583; ENSBTAG00000000459.
GeneIDi497030.
KEGGibta:497030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ868532 mRNA. Translation: CAI30866.1.
BC123822 mRNA. Translation: AAI23823.1.
RefSeqiNP_001011682.1. NM_001011682.2.
XP_005222438.1. XM_005222381.3.
UniGeneiBt.13504.

3D structure databases

ProteinModelPortaliQ5NDF2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000583.

Protein family/group databases

CAZyiGT61. Glycosyltransferase Family 61.

Proteomic databases

PaxDbiQ5NDF2.
PRIDEiQ5NDF2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000000583; ENSBTAP00000000583; ENSBTAG00000000459.
GeneIDi497030.
KEGGibta:497030.

Organism-specific databases

CTDi84892.

Phylogenomic databases

eggNOGiKOG4698. Eukaryota.
ENOG410ZHQA. LUCA.
GeneTreeiENSGT00770000120599.
HOGENOMiHOG000006878.
HOVERGENiHBG054852.
InParanoidiQ5NDF2.
KOiK18207.
OMAiYAVNPDH.
OrthoDBiEOG72G16T.
TreeFamiTF332712.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

NextBioi20865841.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR007657. Glycosyltransferase_AER61.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF04577. DUF563. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Phylogeny of xylosyltransferases."
    Kiefer-Meyer M.C., Pagny S., Durambure G., Faye L., Gomord V., Mollicone R., Oriol R.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Basal ganglia.

Entry informationi

Entry nameiPMGT2_BOVIN
AccessioniPrimary (citable) accession number: Q5NDF2
Secondary accession number(s): Q08DC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: February 1, 2005
Last modified: April 13, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.