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Protein

Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2

Gene

Pomgnt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

O-linked mannose beta-1,4-N-acetylglucosaminyltransferase that transfers UDP-N-acetyl-D-glucosamine to the 4-position of the mannose to generate N-acetyl-D-glucosamine-beta-1,4-O-D-mannosylprotein. Involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] = UDP + 3-O-(N-acetyl-beta-D-glucosaminyl-(1->4)-alpha-D-mannosyl)-L-threonyl-[protein].By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-RNO-5173105. O-linked glycosylation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT61. Glycosyltransferase Family 61.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 (EC:2.4.1.312By similarity)
Short name:
POMGnT2
Alternative name(s):
Extracellular O-linked N-acetylglucosamine transferase-like
Glycosyltransferase-like domain-containing protein 2
Gene namesi
Name:Pomgnt2
Synonyms:Ago61, Gtdc2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1304827. Pomgnt2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini26 – 580LumenalSequence analysisAdd BLAST555

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002490171 – 580Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2Add BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi99N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi276N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5NDF0.
PRIDEiQ5NDF0.

PTM databases

UniCarbKBiQ5NDF0.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019492.
GenevisibleiQ5NDF0. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000063923.

Structurei

3D structure databases

ProteinModelPortaliQ5NDF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini488 – 580Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST93

Sequence similaritiesi

Belongs to the glycosyltransferase 61 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4698. Eukaryota.
ENOG410ZHQA. LUCA.
GeneTreeiENSGT00770000120599.
HOVERGENiHBG054852.
InParanoidiQ5NDF0.
KOiK18207.
OMAiEQGENTY.
OrthoDBiEOG091G03LB.
PhylomeDBiQ5NDF0.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR003961. FN3_dom.
IPR007657. Glycosyltransferase_61.
IPR013783. Ig-like_fold.
PANTHERiPTHR20961. PTHR20961. 1 hit.
PfamiView protein in Pfam
PF04577. DUF563. 1 hit.
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiView protein in PROSITE
PS50853. FN3. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5NDF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLSAVFNAL LVSVLAAVLW KHVRLREHAA TLEEELALGQ QSLDPVPGLR
60 70 80 90 100
IDYPKALQIL MEGGTHMVCT GRTHTDRICR FKWLCYSNEA EEFIFFHGNS
110 120 130 140 150
SVMLPNLGSR RFQPALLDLS TVEDHNAQYF NFVELPAAAL RFMPKPVFVP
160 170 180 190 200
DVALIANRFN PDNLMHVFHD DLLPLFYTLR QFPGLAQEAR LFFMEGWGEG
210 220 230 240 250
AHFDLYKLLS PKQPLLRSQL KTLGRLLCFS HAFVGLSKVT TWYQYGFVQP
260 270 280 290 300
QGPKANILVS GNEIRQFTRF MTERLNVSHA GAPLGEEYIL VFSRTQNRLI
310 320 330 340 350
LNEAELLLEL AQEFQMKTVT VSLEDHTFAD VVRLVSNASM LVSMHGAQLV
360 370 380 390 400
TALFLPRGAT VVELFPYAVN PDHYTPYKTL ATLPGMDLQY VAWRNMIREN
410 420 430 440 450
TVTHPERPWD QGGITHLDRA EQARILQSRE VPRHLCCRNP EWLFRIYQDT
460 470 480 490 500
RVDIPSLMQS IRRVVKGRPG PRRQRWAISL YPGKVREARC QASVQGATEA
510 520 530 540 550
RLSVSWQIPW NLKYLKVREV RYEVWLQEQG ENTYVPYMLT LQNHTFTENI
560 570 580
KPFTTYLVWV RCIFNRSLLG PFADVLVCST
Length:580
Mass (Da):66,717
Last modified:February 1, 2005 - v1
Checksum:i7A444F0927DD63C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ868534 mRNA. Translation: CAI30868.1.
RefSeqiNP_001009437.1. NM_001009437.1.
XP_008764903.1. XM_008766681.2.
XP_008764905.1. XM_008766683.2.
UniGeneiRn.6350.

Genome annotation databases

EnsembliENSRNOT00000026363; ENSRNOP00000063923; ENSRNOG00000019492.
GeneIDi316091.
KEGGirno:316091.

Similar proteinsi

Entry informationi

Entry nameiPMGT2_RAT
AccessioniPrimary (citable) accession number: Q5NDF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: February 1, 2005
Last modified: August 30, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families